Abstract

ABSTRACT

Campylobacter showae a bacterium historically linked to gingivitis and periodontitis, has recently been associated with inflammatory bowel disease and colorectal cancer. Our aim was to generate genome sequences for new clinical C. showae strains and identify functional properties explaining their pathogenic potential. Eight C. showae genomes were assessed, four strains isolated from inflamed gut tissues from paediatric Crohn’s disease patients, three strains from colonic adenomas, and one from a gastroenteritis patient stool. Genome assemblies were analyzed alongside the only 3 deposited C. showae genomes. The pangenome from these 11 strains consisted of 4686 unique protein families, and the core genome size was estimated at 1050 ± 15 genes with each new genome contributing an additional 206 ± 16 genes. Functional assays indicated that colonic strains segregated into 2 groups: adherent/invasive vs. non-adherent/non-invasive strains. The former possessed Type IV secretion machinery and S-layer proteins, while the latter contained Cas genes and other CRISPR associated proteins. Comparison of gene profiles with strains in Human Microbiome Project metagenomes showed that gut-derived isolates share genes specific to tongue dorsum and supragingival plaque counterparts. Our findings indicate that C. showae strains are phenotypically and genetically diverse and suggest that secretion systems may play an important role in virulence potential.

Details

Title
Comparative genomics and genome biology of Campylobacter showae
Author
Hsu, Tiffany 1 ; Gemmell, Matthew R 2 ; Franzosa, Eric A 1 ; Berry, Susan 3 ; Mukhopadhya, Indrani 3 ; Hansen, Richard 4 ; Michaud, Monia 5 ; Nielsen, Hans 6 ; Miller, William G 7 ; Nielsen, Henrik 8 ; Bajaj-Elliott, Mona 9 ; Huttenhower, Curtis 1 ; Garrett, Wendy S 5 ; Hold, Georgina L 10 

1  Department of Biostatistics, Harvard T. H. Chan School of Public Health , Boston , USA 
2  School of Medicine, Medical Sciences and Nutrition, Centre for Genome Enabled Biology and Medicine, University of Aberdeen , Aberdeen , UK 
3  School of Medicine, Medical Sciences and Nutrition, GI Research Group, University of Aberdeen , Aberdeen , UK 
4  Department of Paediatric Gastroenterology, Royal Hospital for Children , Glasgow , UK 
5  Departments of Genetics and Complex Diseases and Immunology and Infectious Diseases, Harvard T. H. Chan School of Public Health , Boston , USA 
6  Department of Clinical Microbiology, Aalborg University Hospital , Aalborg , Denmark 
7  Produce Safety and Microbiology Research Unit, U.S. Department of Agriculture, Agricultural Research Service , Albany , USA 
8  Department of Infectious Diseases, Aalborg University Hospital Aalborg , Denmark 
9  Infection, Immunity, Inflammation Programme, UCL Great Ormond Street Institute of Child Health , London , UK 
10  Department of Biostatistics, Harvard T. H. Chan School of Public Health , Boston , USA, School of Medicine, Medical Sciences and Nutrition, GI Research Group, University of Aberdeen , Aberdeen , UK, Departments of Genetics and Complex Diseases and Immunology and Infectious Diseases, Harvard T. H. Chan School of Public Health , Boston , USA, St George and Sutherland Clinical School, Microbiome Research Centre, University of New South Wales , Sydney , Australia 
Pages
827-840
Publication year
2019
Publication date
2019
Publisher
Taylor & Francis Ltd.
e-ISSN
22221751
Source type
Scholarly Journal
Language of publication
English
DOI
https://doi.org/10.1080/22221751.2019.1622455
ProQuest document ID
2427525771
Copyright
© 2019 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group, on behalf of Shanghai Shangyixun Cultural Communication Co., Ltd. This work is licensed under the Creative Commons Attribution License http://creativecommons.org/licenses/by/4.0/ (the "License"). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.