Abstract

Background

Acetyl octapeptide-3 (SNAP-8) is an antiaging peptide that is more effective than acetyl hexapeptide-3, which is more stable than botulinum toxin and effectively relieves facial wrinkles. Products containing SNAP-8 such as patch are producing, but analytical method has not been reported to determination of SNAP-8.

Method

Mobile phase, collision energy, and desolvation line temperature were optimized, and mass spectral data set for SNAP-8 was newly constructed by liquid chromatography-triple quadrupole mass spectrometer in multiple reaction monitoring mode.

Results

The developed method showed good linearity (r ≥ 0.9971) with limit of quantification of 0.0125 ng/mL, repeatability (% relative standard deviation = 0.02 to 0.12) and accuracy (% relative error = − 1.68 to 1.44) under optimal conditions. This method was successfully applied to a biodegradable microneedle patch loaded with SNAP-8.

Conclusion

The present method for the quantification of SNAP-8 may be useful for quality control in the cosmetic fields.

Details

Title
Method development for acetyl octapeptide-3 analysis by liquid chromatography-tandem mass spectrometry
Author
Ji Moongi 1 ; Hyeon-Seong, Lee 1 ; Kim, Youngbae 1 ; Chan, Seo 2 ; Choi Subin 3 ; Oh Songjin 1 ; Jeuk, Min 1 ; Hyung-Jin, Park 1 ; Kim Jung Dong 4 ; Jeong Do Hyeon 4 ; Man-Jeong, Paik 1 

 Sunchon National University, College of Pharmacy, Suncheon, Republic of Korea (GRID:grid.412871.9) (ISNI:0000 0000 8543 5345) 
 Daegu-Gyeongbuk Medical Innovation Foundation, New Drug Development Center, Daegu, Republic of Korea (GRID:grid.496160.c) (ISNI:0000 0004 6401 4233) 
 Redone Tech, Laboratories of Marine New Drugs, Seoul, Republic of Korea (GRID:grid.496160.c) 
 Avison Biomedical Research Center, Raphas, Seoul, Republic of Korea (GRID:grid.412871.9) 
Publication year
2020
Publication date
Dec 2020
Publisher
Springer Nature B.V.
ISSN
20933134
e-ISSN
20933371
Source type
Scholarly Journal
Language of publication
English
ProQuest document ID
2433064106
Copyright
© The Author(s) 2020. This work is published under http://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.