Content area
Background
The present study was designed to characterize phenotypically and genotypically two Trueperella pyogenes strains isolated from an okapi (Okapia johnstoni) and a royal python (Python regius).
Case presentation
The species identity could be confirmed by phenotypic properties, by MALDI-TOF MS analysis and by detection of T. pyogenes chaperonin-encoding gene cpn60 with a previously developed loop-mediated isothermal amplification (LAMP) assay. Furthermore, sequencing of the 16S ribosomal RNA (rRNA) gene, the 16S-23S rDNA intergenic spacer region (ISR), the target genes rpoB encoding the β-subunit of bacterial RNA polymerase, tuf encoding elongation factor tu and plo encoding the putative virulence factor pyolysin allowed the identification of both T. pyogenes isolates at species level.
Conclusions
Both strains could be clearly identified as T. pyogenes. The T. pyogenes strain isolated in high number from the vaginal discharge of an okapi seems to be of importance for the infectious process; the T. pyogenes strain from the royal python could be isolated from an apparently non-infectious process. However, both strains represent the first isolation of T. pyogenes from these animal species.
Details
Infections;
Vagina;
rRNA 23S;
Species;
RpoB protein;
RNA polymerase;
rRNA 16S;
Gene amplification;
Investigations;
Spacer region;
Elongation factor EF-Tu;
Bacteriology;
Pyolysin;
Hogs;
Abortion;
Strains (organisms);
DNA-directed RNA polymerase;
Virulence factors;
Trueperella pyogenes;
Python regius;
Okapia johnstoni