Schistosome migration in the definitive host

Abstract

Schistosomes are parasitic blood flukes that infect >200 million people around the world. Free-swimming larval stages penetrate the skin, invade a blood vessel, and migrate through the heart and lungs to the vasculature of the liver, where maturation and mating occurs. From here, the parasite couples migrate to their preferred egg laying sites. Here, we compare and contrast what is known about the migration patterns within the definitive host of the three major species of human schistosome: Schistosoma mansoni, S. japonicum, and S. haematobium. We conclude that intravascular schistosomes are inexorable colonizers whose migration and egg laying strategy is profligate; all three species (and their eggs) can be found throughout the mesenteric venules, the rectal venous plexus, and, to a greater or lesser extent, the urogenital venous plexuses. In addition, it is common for parasite eggs to be deposited in locations that lack easy access to the exterior, further demonstrating the relentless exploratory nature of these intravascular worms.

About the Authors:

Catherine S. Nation

Affiliation: Department of Infectious Disease and Global Health, Cummings School of Veterinary Medicine, Tufts University, North Grafton, Massachusetts, United States of America

Akram A. Da’dara

Affiliation: Department of Infectious Disease and Global Health, Cummings School of Veterinary Medicine, Tufts University, North Grafton, Massachusetts, United States of America

Jeffrey K. Marchant

Affiliation: Department of Medical Education, Tufts University School of Medicine, Boston, Massachusetts, United States of America

ORCID logo http://orcid.org/0000-0002-2401-6010

Patrick J. Skelly

* E-mail: [email protected]

Affiliation: Department of Infectious Disease and Global Health, Cummings School of Veterinary Medicine, Tufts University, North Grafton, Massachusetts, United States of America

ORCID logo http://orcid.org/0000-0002-5733-0524

Abstract

Schistosomes are parasitic blood flukes that infect >200 million people around the world. Free-swimming larval stages penetrate the skin, invade a blood vessel, and migrate through the heart and lungs to the vasculature of the liver, where maturation and mating occurs. From here, the parasite couples migrate to their preferred egg laying sites. Here, we compare and contrast what is known about the migration patterns within the definitive host of the three major species of human schistosome: Schistosoma mansoni, S. japonicum, and S. haematobium. We conclude that intravascular schistosomes are inexorable colonizers whose migration and egg laying strategy is profligate; all three species (and their eggs) can be found throughout the mesenteric venules, the rectal venous plexus, and, to a greater or lesser extent, the urogenital venous plexuses. In addition, it is common for parasite eggs to be deposited in locations that lack easy access to the exterior, further demonstrating the relentless exploratory nature of these intravascular worms.

Citation: Nation CS, Da’dara AA, Marchant JK, Skelly PJ (2020) Schistosome migration in the definitive host. PLoS Negl Trop Dis 14(4): e0007951. https://doi.org/10.1371/journal.pntd.0007951

Editor: Stephen Davies, University of Colorado Denver, UNITED STATES

Published: April 2, 2020

Copyright: © 2020 Nation et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Funding: This work was supported by grant AI-056273 from the NIH-NIAID to PJS, https://www.niaid.nih.gov/. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.

Competing interests: The authors have declared that no competing interests exist.

Schistosomes are parasitic blood flukes that infect over 200 million people around the world. Infection occurs when free-swimming larval stages called cercariae penetrate the epidermis. Once inside the human body, the young worms undertake a remarkable journey, first from the skin to a blood vessel, then through the heart and lungs to the vasculature of the liver, where maturation and mating occurs. From here, the parasite couples migrate to their preferred egg laying sites. Here, we review what is known about the migration patterns within the definitive host of the three major species of human schistosome: S. mansoni, S. japonicum, and S. haematobium.

Migration from the skin to the liver

For all species, infection begins when the cercariae breach the epidermis, a process that can occur within minutes [1], though parasites can reside in the skin for several hours or even days [2–5]. In the skin, the parasites transform morphologically and biochemically into juvenile forms called schistosomula; these migrate into and through the dermis, eventually reaching dermal blood vessels. S. mansoni and S. haematobium schistosomula exit the skin and reach blood vessels by approximately 3 days postinfection [2,4–6], whereas S. japonicum schistosomula complete the process sooner; as early as 2 hours postinfection, these schistosomula are found in dermal blood vessels and, in one study, by 8 hours, 73% had migrated through the dermis [5,7–9].

Schistosomula in the circulation reach the lungs via the right side of the heart and pulmonary artery [2]. Within the capillary beds of the lungs, the young parasites elongate to facilitate passage through these smaller blood vessels [10,11]. In the mouse, S. mansoni schistosomula begin to arrive in the lungs between 2 and 3 days postinfection, peaking at around day 7 and lasting until around day 11 [4,12,13]. However, rates of migration may differ in different definitive hosts. For instance, in the olive baboon, S. mansoni schistosomula migrate much faster to the lungs and liver than in mice, with the majority of schistosomula passing through the lungs and reaching the baboon liver by day 9 [14]. Radiotracking studies indicate that S. haematobium reach the lungs at around day 7 and can be present there up to 25 days postinfection [15]. S. japonicum can be recovered from lung tissues at day 2, peaking at day 3 [9,16,17].

From the lungs, schistosomula can flow with the blood via the pulmonary veins through the left side of the heart, and then they can become distributed to the systemic organs. Parasites that pass from the descending (abdominal) aorta into the celiac trunk can reach the liver either directly (via the hepatic artery) or by passing through the stomach (left and right gastric arteries), small intestine (gastroduodenal artery), or spleen or pancreas (splenic artery) to reach the hepatic portal vein. Fig 1 indicates the path of migration of a parasite that has invaded the skin, entered the vasculature of the leg, and taken this path (route A). Parasites that enter the superior mesenteric artery or the inferior mesenteric artery can also pass through the intestinal blood vessels to, again, expeditiously reach the portal vein and the liver (Fig 1, route B). It is also possible that schistosomula that drain to the blood vessels around the bladder or reproductive organs (from the gonadal artery, or branches of the internal iliac artery) could make their way to the vasculature of the intestines (route C) and from here to the liver. However, parasites that do not flow into these arteries get carried in the blood stream elsewhere; they circulate throughout the systemic organs [12,15,18,19] before eventually returning to the lungs to repeat the circuit. This may occur multiple times until they are lucky enough to be washed into the splanchnic arteries (i.e., the celiac or superior/inferior mesenteric arteries) and through these, to the liver [2,4,10,20,21].

[Figure omitted. See PDF.]

Fig 1. Diagrammatical representation of core mammalian vasculature showing (white arrows) possible routes of migration of a schistosome juvenile to the portal vasculature, where the parasites mature.

A cercaria (depicted at bottom) has penetrated the skin of the lower body and invaded the vasculature, as suggested by the black arrow. White arrows track the parasite travelling via the inferior vena cava to the heart, then to the left lung before returning to the heart and entering the abdominal aorta. From the aorta, pathway “A” indicates parasites entering the celiac trunk to the gastroduodenal, hepatic, or splenic arteries to the liver. Pathway “B” indicates parasites entering the superior or inferior mesenteric arteries and moving through the hepatic portal vessels to the liver. Pathway “C” depicts parasites entering the internal iliac artery to the vessels of the bladder, from where they could reach the liver via the superior rectal and hepatic portal vasculature. Arteries are depicted in red, most veins in blue; hepatic portal veins in purple. Fig 1 was created with Biorender.com.

https://doi.org/10.1371/journal.pntd.0007951.g001

Migration beyond the liver

Once at the liver, schistosomula are primarily observed in the branches of the hepatic portal vein and are seemingly unable to pass through the sinusoids and back into the systemic blood flow [4,12,20]. As observed by direct microscopic observation of parasites in situ in the vasculature of anesthetized mice whose abdominal blood vessels have been exposed by surgery, in the portal vein the young worms begin feeding on blood and are noted to be either attached to vessels via the ventral sucker or wedged into a vessel, facing the direction of blood flow [12]. Here, the parasites mature—doubling in weight approximately every 2.3 days in the first 2 weeks [21]. Next, the worms find a mate and the pairs migrate from the liver against the flow of blood to reach their preferred egg laying sites.

S. mansoni

To transmit their eggs, S. mansoni adult pairs migrate to the venous vasculature around the intestines; eggs laid here can pass through the blood vessel wall, through the wall of the intestine, and into the intestinal lumen. From here, eggs exit the body with feces. Fig 2 shows that the adult S. mansoni worms in the blood vessels of the liver are presented with some choices when migrating to such egg laying sites in the mesenteries. For instance, from the hepatic portal vein the worm pairs (taking route #1, Fig 2) could enter the splenic vein, then turn into the inferior mesenteric vein, then move down either the left colic vein or one of the sigmoidal veins. The distal venules derived from these vessels are intimately associated with the descending and sigmoid colon, and parasite eggs laid in these locations could pass through the blood vessel wall, through the wall of the intestine, and into the intestinal lumen. Alternatively, the adults could migrate from the hepatic portal vein into the superior mesenteric vein, then deviate down the right colic or ileocolic veins to lay eggs in the venules of the ascending colon (route #2). Worms could also travel to the transverse colon through the middle colic vein (not depicted).

[Figure omitted. See PDF.]

Fig 2. Diagrammatical representation of the hepato-portal and associated vasculature showing possible routes of migration (yellow arrows) of schistosomes from the blood vessels of the liver to their egg laying sites.

From the hepatic portal vein, pathway “1” indicates parasites entering the splenic vein, then the inferior mesenteric vein to the venules of the descending colon. Pathway “2” indicates parasites entering the superior mesenteric vein to right colic vein and the venules of the ascending colon. Pathway “3” indicates parasites leaving the superior mesenteric vein via the jejunal and ileal veins to the venules draining the small intestine (jejunum and ilium). Pathway “4” indicates parasites traveling via the inferior mesenteric vein into the superior rectal (hemorrhoidal) vein to the venules of the rectum. Finally, pathway “5” indicates parasites migrating beyond the vasculature of the rectum via the rectal venous plexus through the uterine and vaginal venous plexuses (in females, as illustrated, or the prostatic venous plexus in males, not shown) into the vesicle venous plexus draining the bladder. Image credit: Thanks to Taoli Shen for illustrating Fig 2.

https://doi.org/10.1371/journal.pntd.0007951.g002

From the superior mesenteric vein, the parasites could otherwise move via the jejunal and ileal veins to the venules draining the small intestine (jejunum and ilium) to lay eggs (route #3). It is clear that S. mansoni adults avail of all of these pathways because parasite eggs are deposited throughout the entire length of the intestinal tract of infected experimental animals [22–24]. Indeed, this is the case even in mice infected with just a single pair of worms; this means that worm pairs move extensively all along the complex venous system, draining the murine intestinal tract [25]. In addition, using in vivo microscopy, worms have been directly observed to move constantly in the mesenteric veins of their murine hosts [12].

Fig 3A shows adult S. mansoni worms (indicated by the arrows) in the blood vessels of the exposed mesenteries of a 7-week infected mouse. Fig 3B is a hematoxylin and eosin–stained longitudinal section of a schistosome-infected mouse blood vessel showing the female worm within the gynaecophoric canal of the male. This image highlights the snugness of fit of the parasite couple; the vascular endothelium surrounding the worms is indicated by arrows.

[Figure omitted. See PDF.]

Fig 3.

(A) Exposed mesenteries of a 7-week infected female Swiss Webster mouse showing intravascular adult S. mansoni worms (indicated by arrows). (B) Hematoxylin and eosin–stained, formalin-fixed section of a blood vessel of a 7-week infected female Balb/c mouse containing an adult S. mansoni couple. The male worm and his female partner (within his gynaecophoric canal) are both indicated. The vascular endothelium bounding the worm couple is indicated by arrows.

https://doi.org/10.1371/journal.pntd.0007951.g003

Examination of infected humans at autopsy reveals that the adult worms, as in mice, are widely distributed in the colonic venules, in the small intestinal venules, and in the liver and portal vein [26]. While the inferior mesenteric vein is considered the “natural habitat” of S. mansoni, this habitat actually extends to all veins that drain into the hepatic portal system [27]. In keeping with this wide distribution of the worms seen at autopsy, parasite eggs are also found throughout the human intestinal mucosa, with higher proportions of the eggs being reported in the rectum and colon versus the small intestine (ilium and jejunum) [26,28,29]. The presence of S. mansoni eggs in high numbers in the rectum confirms that these worms can migrate down the superior rectal vein to the anorectal plexus to lay eggs (as depicted in Fig 2, route #4) and are by no means confined to the proximal mesenteric veins [27]. Indeed, S. mansoni eggs have been detected even in the bladder and in the urine of infected individuals, showing that the worms can move beyond the anorectal plexus to the vesicle venous plexus (a migration more commonly associated with S. haematobium, see later) [28,30,31]. It has been reported that S. mansoni eggs are excreted in the urine more often in mixed S. mansoni/S. haematobium infections versus in pure S. mansoni infections; S. mansoni eggs have been detected in the urine of 5.6% of people (62/1,146) with a mixed infection but only in 0.7% of people (6/840) with a pure S. mansoni infection [32,33]. However, in another study of 106 pure S. mansoni–infected individuals, a notably higher proportion—14.5% (15 patients)—were found to pass S. mansoni eggs in the urine [30].

Demonstrating the relentless exploratory nature of intravascular schistosomes, parasite eggs have been detected in the spleen, the stomach, the pancreas, and gall bladder of infected humans [28,29,34,35]. Because venous blood flows away from these organs, eggs found there are not passively swept to these sites. The worms actively moved, for instance, to the terminus of the splenic vein and laid eggs there, even though these eggs have no route to the outside to continue the parasite’s life cycle. This finding of wide egg deposition raises the question as to whether S. mansoni females lay eggs directly in the veins of the liver versus, as is usually assumed, that eggs in the liver arrived there by being swept back to this site with the blood flow after having been laid elsewhere. Indeed, the worm couples could potentially begin laying eggs in the portal vessels as soon as they pair and while they are in the process of migrating away from the liver.

Worm distribution can change over the course of infection. For instance, prolonged infection in rhesus macaques (>187 days post-cercarial exposure) is accompanied by a marked shift of S. mansoni worms from colonic venules to venules of the small intestine [36]. The change in worm position is akin to that seen in human subjects with Symmers fibrosis of the liver (a condition not seen in macaques) [26].

S. japonicum

S. japonicum infects several animal species and causes zoonotic disease; the distribution of intravascular worms of this species has been examined in a variety of animals. In all cases, the parasites are found widely distributed throughout the mesenteries (reviewed in [37]), showing that the adult pairs (like S. mansoni) can also navigate via routes 1, 2, 3, and 4 as depicted in Fig 2. Based on the distribution of S. japonicum eggs and the severity of pathological changes along the length of the intestine, there is debate as to the relative importance of these routes in different host species; higher egg density and/or more severe pathology in the small intestine is reported in smaller animal hosts (for instance, mouse, guinea pig [38,39]—suggesting a preference for route 3), whereas higher egg density and/or more severe pathology in the large intestine is reported in larger animal hosts (for instance, goat, sheep, buffalo [38]—suggesting a preference for routes 1 and 2). Humans (large animal hosts) follow this trend; adult worms are localized predominantly in the inferior mesenteric vein and the superior rectal vein and egg numbers are highest in the rectum, the sigmoid colon, and the descending colon [37,40]. As is the case for S. mansoni, infection of mice with a single S. japonicum worm pair also results in parasite eggs scattered throughout the gut, indicating movement of these worms in the venous vasculature along the length of the intestine [41].

Also as seen for S. mansoni, egg deposition in “non-productive” sites such as the spleen and stomach is seen with S. japonicum [40,42]. In addition, there are case reports documenting S. japonicum eggs in the ovary [43], fallopian tube [44], or prostate [45], showing that sometimes S. japonicum worms too can migrate beyond the mesenteric venules [46].

The situation is complicated by the existence of different S. japonicum strains [42]. In one study in rabbits, the preferred oviposition site for a Japanese schistosome strain was found to be the small intestine, whereas the large intestine was preferred in infections with a Philippine strain [47]. In contrast, S. japonicum eggs were found predominantly in the small intestine in capuchin monkeys infected with the Philippine strain and predominantly in the colon in monkeys infected with the Japanese strain [48].

S. haematobium

S. haematobium, unlike the other two major human-infecting schistosome species, migrates primarily to the veins surrounding the bladder (the vesicle venous plexus) and causes urinary schistosomiasis. The route taken is not often described, but Fig 2 (route #5) shows one relatively direct path of migration for the adult pairs from the hepatic portal vein: first into the splenic vein, then into the inferior mesenteric vein. This vein runs directly into the superior rectal (hemorrhoidal) vein, which leads to a collection of interconnected vessels known as the rectal venous plexus. This plexus connects with another series of interconnected vessels draining the reproductive organs (the uterine and vaginal venous plexuses in females [illustrated in Fig 2] and the prostatic venous plexus in males) which, in turn, connect with the network of vessels draining the bladder (the vesicle venous plexus). The abundant interconnections (anastomoses) between these different venous plexuses in the pelvis facilitate worm movement. These vessels largely lack valves and so offer a network of unrestricted routes for the worms to, and through, the urogenital organ systems. Schistosome eggs laid in the vesicle venous plexus can traverse the blood vessel wall, as well as the wall of the bladder, to enter the lumen of the bladder and can then exit the body with urine. In keeping with this, most of the S. haematobium adult worms seen at autopsy are found in the blood vessels of the bladder, and the highest percentage of eggs in the organs are likewise found in the bladder (followed by the ureter) [31]. Egg distribution is observed to be uneven in the lower urinary tract (i.e., bladder and urethra) and focal egg deposition is considered to be more characteristic of light infections, while heavy worm burdens result in eggs being laid more evenly throughout the region [49].

S. haematobium adults, however, are by no means confined to the urinary tract. In one study examining 46 cases at autopsy, adult S. haematobium were located in roughly equal proportions in the mesenteric and portal veins (47%) compared to the bladder (48%) [31] and, in keeping with this distribution, considerable numbers of S. haematobium eggs were found in the colon [31], a finding that is commonly observed in humans [28,29,34] and in a variety of primates [50]. While a majority of S. haematobium eggs in the intestine are seen in the rectum, the fact that eggs are also located in the cecum, appendix, and small intestine demonstrates that S. haematobium too can migrate down the superior mesenteric vein from the liver (Fig 2, route #3)—a route from which the worms cannot progress directly to the bladder (Fig 2) [28,29,31,35,51].

Fig 2 shows the extensive interconnected network of veins in the pelvis through which the parasites can migrate. It appears that the worms lay eggs throughout this network even though, in many cases, there is little prospect for those eggs to exit the body and continue the parasite’s life cycle. For instance, in infected women, eggs can be found in the cervix, uterus, vagina, fallopian tubes, and ovaries, all contributing to the widespread pathology collectively called female genital schistosomiasis [31,35,52–54], and all essentially dead-end sites for the worms in terms of parasite transmission. In a meta-analysis of the anatomical distribution of female genital schistosomiasis based on histopathological and postmortem studies in various endemic areas, the worms displayed no strong and consistent preference for a specific female genital organ [55]. For instance, of all eggs found in the genital organs, the percentage found in the cervix varied from 7% to 62% in different studies; the percentage found in the fallopian tubes varied from 7% to 32%, and in the vagina from 10% to 78% [55].

In men, S. haematobium eggs can be found at autopsy in the epididymis, prostate, seminal vesicles, vas deferens, and testes [31,34,35,52,56], leading to male genital schistosomiasis with, as in females, little prospect for easy egg egress from the body from these genital sites. As in women, in men, too, the worms show no strong preference for localization in any specific genital site. Eggs in the prostate have been reported from 14% to 100% of cases, and eggs in the seminal vesicles from 55% to 100% [52,57].

Emphasizing once more the relentless migratory tendencies of schistosomes, note that in cases of S. mansoni infection too, parasite eggs can sometimes be found in all of the female and male genital organs just listed but, in comparison with S. haematobium infection, in much reduced numbers [31,57].

Finally, it is worth noting that once schistosomes reach the anorectal plexus there is relatively easy access to systemic venous blood flow from the middle and inferior rectal veins, or the pelvic venous system to the inferior vena cava. Eggs that distribute into systemic blood circulation via these routes can get trapped, for instance, in the capillary beds of the lungs [58,59]. In one histological autopsy study, 59% of 108 cases had eggs in the lungs, 94% of these being S. haematobium (and the remainder mixed S. haematobium and S. mansoni). Here, S. mansoni was only found in the lungs when the bladder was affected [28]. Eggs of all species, and indeed adult worms, can also be found in a variety of other tissues, including the brain and spinal cord, with S. japonicum—the most prolific egg producer among the human schistosomes—being more commonly found in the brain [28,34,40,60].

A note about schistosome eggs and egg laying

Schistosomes are remarkably fecund: S. mansoni worm pairs can lay >300 eggs per day and S. japonicum pairs >2,000 eggs per day [61]. Egg morphology is diagnostic for each species: The S. mansoni egg has a prominent lateral spine; that of S. haematobium presents a terminal spine, while the spine of S. japonicum is rudimentary. All eggs contain pores, and thin microfilaments (microspines) coat their surfaces (reviewed in [62]). Eggs released by schistosomes must cross the blood vessel walls of the intestines or the urinary tract in order to complete the life cycle. This egg extravasation process is promoted by endothelial activation as well as interactions with blood clotting components (reviewed in [63]). About a third to half of the eggs produced do not reach the external environment; instead they become trapped in tissues of the host without direct access to the exterior. Here, the eggs evoke a strong inflammatory response; they become surrounded by a barrage of immune cells, generating a structure called a granuloma, and this forms the basis for much of the pathology of schistosomiasis. In intestinal tissue, granuloma formation is considered important for the process of egg transit [64]. When first laid by female worms, schistosome eggs are immature; over the following approximately 7 days they enlarge and develop to contain viable and fully formed larvae (called miracidia) [65]. Outside the body, the miracidia hatch from the eggs in freshwater and seek a suitable snail to act as an intermediate host to continue the life cycle.

Concluding remarks

It is clear that intravascular schistosomes are inexorable colonizers whose migration and egg laying strategy is profligate; the adult worms and their eggs can be commonly found throughout the mesenteric venous system and the pelvic venous plexuses. In the case of S. mansoni, the worms are largely in the mesenteries; for S. japonicum the worms appear to be almost exclusively in the mesenteries. Both of these species cause intestinal schistosomiasis. In the case of S. haematobium, a high proportion of worms and eggs tend to be found in the vesicle plexuses of the pelvis, causing urinary and genital schistosomiasis. Note that in all cases regarding the descriptions of the location of worms in humans in the older literature, we assume that the patients were at no time exposed to subcurative chemotherapy, which could, of course, impact worm distribution.

Outstanding questions remain: What, for example, drives adult schistosome migration from the liver to egg laying sites in the first place? As noted, the three major schistosome species of humans have overlapping intravascular habitats, yet the adults of each species tend to be found in preferred niches; why, for instance, are a high proportion of S. haematobium worms and eggs located in the vesicle venous plexus? Which host molecules guide migration, and what selective pressures impact parasite egg laying site preferences? Is the presence of parasite eggs in locations that lack easy access to the exterior accidental, and does this suggest that some parasites have poor migratory skills? Finally, is the mechanism of egg movement into the lumen of the GI tract the same throughout the length of the gut (and in the bladder)?

Citation: Nation CS, Da’dara AA, Marchant JK, Skelly PJ (2020) Schistosome migration in the definitive host. PLoS Negl Trop Dis 14(4): e0007951. https://doi.org/10.1371/journal.pntd.0007951

References

1. Stirewalt MA. Schistosoma mansoni: cercaria to schistosomule. Adv Parasitol. 1974;12: 115–182. pmid:4141581

2. Miller P, Wilson RA. Migration of the schistosomula of Schistosoma mansoni from the lungs to the hepatic portal system. Parasitology. 1980;80: 267–288. pmid:7367042

3. Crabtree JE, Wilson RA. Schistosoma mansoni: an ultrastructural examination of skin migration in the hamster cheek pouch. Parasitology. 1985;91: 111–120. pmid:4034241

4. Wheater PR, Wilson RA. Schistosoma mansoni: a histological study of migration in the laboratory mouse. Parasitology. 1979;79: 49–62. pmid:542321

5. He YX, Chen L. Schistosoma mansoni, S. haematobium, and S. japonicum: early events associated with penetration and migration of schistosomula through human skin. Exp Parasitol. 2002;102: 99–108. pmid:12706745

6. Miller P, Wilson RA. Migration of the schistosomula of Schistosoma mansoni from skin to lungs. Parasitology. 1978;77: 281–302. pmid:748837

7. Wang L, Li Y-L, Fishelson Z, Kusel JR, Ruppel A. Schistosoma japonicum migration through mouse skin compared histologically and immunologically with S. mansoni. Parasitol Res. 2005;95: 218–223. pmid:15723270

8. He Y-X, Salafsky B, Ramaswamy K. Comparison of skin invasion among three major species of Schistosoma. Trends Parasitol. 2005;21: 201–203. pmid:15837605

9. Sadun EH, Williams JE. Studies on the host parasite relationships to Schistosoma japonicum. I. The effect of single graded infection and the route of migration of schistosomula. Am J Trop Med Hyg. 1958;7: 494–499. pmid:13571564

10. Wilson RA, Coulson PS. Schistosoma mansoni: dynamics of migration through the vascular system of the mouse. Parasitology. 1986;92: 83–100. pmid:3083394

11. Rheinberg CE, Moné H, Caffrey CR, Imbert-Establet D, Jourdane J, Ruppel A. Schistosoma haematobium, S. intercalatum, S. japonicum, S. mansoni, and S. rodhaini in mice: relationship between patterns of lung migration by schistosomula and perfusion recovery of adult worms. Parasitol Res. 1998;84: 338–342. pmid:9569102

12. Bloch EH. In vivo microscopy of schistosomiasis. Am J Trop Med and Hyg. 1980;29: 62–70.

13. Georgi JR, Dean DA, Mangold BL. Schistosoma mansoni: temporal distribution of radioselenium-labelled schistosomula in lungs of mice during the first two weeks of infection. Parasitology. 1983;86: 31–36. pmid:6835697

14. Wilson RA, Coulson PS, Sturrock RF, Reid GD. Schistosome migration in primates: a study in the olive baboon (Papio anubis). Trans R Soc Trop Med Hyg. 1990;84: 80–83. pmid:2111948

15. Georgi JR, Wade SE, Parasitology DD. Attrition and temporal distribution of Schistosoma mansoni and S. haematobium schistosomula in laboratory mice. Parasitology. 1986;93: 55–70. pmid:3092171

16. Gui M, Kusel JR, Shi YE, Ruppel A. Schistosoma japonicum and S. mansoni: comparison of larval migration patterns in mice. J Helminthol. 1995;69: 19–25. pmid:7622787

17. Olivier L. A comparison of infections in mice with three species of schistosomes, Schistosoma mansoni, Schistosoma japonicum and Schistosomatium douthitti. Am J Hyg 1952;50, 22–35

18. Georgi JR, Wade SE, Dean DA. Schistosoma mansoni: Mechanism of attrition and routes of migration from lungs to hepatic portal system in the laboratory mouse. J Parasitol. 1987;73: 706. pmid:3114464

19. Dean DA, Mangold BL, Georgi JR, Jacobson RH. Comparison of Schistosoma Mansoni migration patterns in normal and irradiated cercaria-immunized mice by means of autoradiographic analysis. Am J Trop Med Hyg. 1984;33: 89–96. pmid:6696190

20. Wilson RA, Draskau T, Miller P, Lawson JR. Schistosoma mansoni: the activity and development of the schistosomulum during migration from the skin to the hepatic portal system. Parasitology. 1978;77: 57–73. pmid:756526

21. van Oordt BEP, Tielens AGM, van den Bergh SG. The energy metabolism of Schistosoma mansoni during its development in the hamster. Parasitol Res. 1988;75: 31–35. pmid:3144712

22. Bloch EH. In vivo microscopy of schistosomiasis. IV. The dynamics of the host-parasite responses to Schistosoma mansoni in the hypodermal tissues as observed in transparent chambers in two susceptible hosts during primary and challenge infections. Am J Trop Med Hyg. 1984;33: 899–910. pmid:6486299

23. Moore DV, Sandground JH. The relative egg producing capacity of Schistosoma mansoni and Schistosoma japonicum. Am J Trop Med Hyg. 1956;5: 831–840. pmid:13362750

24. Sadun EH, Lichtenberg Von F, Hickman RL, Bruce JI, Smith JH, Schoenbechler MJ. Schistosomiasis mansoni in the chimpanzee: parasitologic, clinical, serologic, pathologic and radiologic observations. Am J Trop Med Hyg. 1966;15: 496–506. pmid:5941172

25. Pellegrino J, Coelho PMZ. Schistosoma mansoni: wandering capacity of a worm couple. J Parasitol. 1978;64: 181. pmid:627964

26. Cheever AW. A quantitative post-mortem study of Schistosomiasis mansoni in man. Am J Trop Med Hyg. 1968;17: 38–64. pmid:5637021

27. Dew HR. Observations on the pathology of schistosomiasis (S. haematobium and S. mansoni) in the human subject. J Pathol Bacteriol. 1923;26: 27–39.

28. Gelfand M. Schistosomiasis in South Central Africa: A Clinico-Pathological Study. Cape Town and Johannesburg: The Post-Graduate Press; 1950. https://doi.org/10.1001/jama.1950.02920140080040

29. Gelfand M, Ross WF. I. The distribution of schistosome ova in the alimentary tract in subjects of bilharziasis. Trans R Soc Trop Med Hyg. 1953;47: 215–217. pmid:13077720

30. Cook JA, Jordan P. Excretion of Schistosoma mansoni ova in the urine. Trans R Soc Trop Med Hyg. 1970;64: 793–794. pmid:5533335

31. Cheever AW, Kamel IA, Elwi AM, Mosimann JE, Danner R. Schistosoma mansoni and S. haematobium infections in Egypt. II. Quantitative parasitological findings at necropsy. Am J Trop Med Hyg. 1977;26: 702–716. pmid:889013

32. Blair DM. The routes of Schistosome egg passage from the body. Cent Afr J Med. 1965;11: 243–249. pmid:5832456

33. Husting EL. Comments on "the routes of schistosome egg passage …". Cent Afr J Med. 1965;11: 250–254. pmid:5833090

34. Alves W. The distribution of Schistosoma eggs in human tissues. Bull World Health Organ. World Health Organization; 1958;18: 1092–1097. pmid:13573135

35. Edington GM, Nwabuebo I, Junaid TA. The pathology of schistosomiasis in Ibadan, Nigeria with special reference to the appendix, brain, pancreas and genital organs. Trans R Soc Trop Med Hyg. 1975;69: 153–162. pmid:1145708

36. Cheever AW, Powers KG. Schistosoma mansoni infection in rhesus monkeys: changes in egg production and egg distribution in prolonged infections in intact and splenectomized monkeys. Ann Trop Med Parasitol. 1969;63: 83–93. pmid:4980588

37. Chen MG. Relative distribution of Schistosoma japonicum eggs in the intestine of man: a subject of inconsistency. Acta Tropica. 1991;48: 163–171. pmid:1671618

38. Ho YH. On the Host Specificity of Schistosoma japonicum. Chin Med J. 1963;82: 405–414. pmid:14057765

39. Hirose Y, Matsumoto J, Kirinoki M, Shimada M, Chigusa Y, Nakamura S, et al. Schistosoma mekongi and Schistosoma japonicum: differences in the distribution of eggs in the viscera of mice. Parasitol Int. 2007;56: 239–241. pmid:17521955

40. World Health Organization. Schistosomiasis and other Trematode Infections Unit. Progress in assessment of morbidity due to Schistosoma japonicum infection: a review of recent literature. World Health Organization. 1988 [cited 2019 Dec 19]. Available from: https://apps.who.int/iris/handle/10665/62414

41. Cheever AW, Duvall RH. Schistosoma japonicum: migration of adult worm pairs within the mesenteric veins of mice. Trans R Soc Trop Med Hyg. 1982;76: 641–645. pmid:7179417

42. Hsü HF, Hsü SYL. Distribution of eggs of different geographic strains of Schistosoma japonicum in the viscera of infected hamsters and mice. Am J Trop Med Hyg. 1960;9: 240–247. pmid:14403600

43. Lee KF, Hsueh S, Tang MH. Schistosomiasis of the ovary with endometriosis and corpus hemorrhagicum: a case report. Chang Gung Med J. 2000;23: 438–441. pmid:10974760

44. Carpenter CB, Mozley PD, Lewis NG. Schistosomiasis japonica involvement of the female genital tract. JAMA. 1964;188: 647–650. pmid:14121286

45. Yu Z, Wei C, Wang Y, Ye Z, Wang Z, Chen Z, et al. Prostatic Schistosoma japonicum with atypical immunophenotyping of individual glandular tubes: a case report and review of the literature. Southeast Asian J Trop Med Public Health. 2013;44: 568–573. pmid:24050089

46. Feldmeier H, Daccal RC, Martins MJ, Soares V, Martins R. Genital manifestations of schistosomiasis mansoni in women: important but neglected. Mem Inst Oswaldo Cruz. 1998;93: 127–133.

47. Cheever AW, Duvall RH, Minker RG. Quantitative parasitologic findings in rabbits infected with Japanese and Philippine strains of Schistosoma japonicum. Am J Trop Med Hyg. 1980;29: 1307–1315. pmid:7446822

48. Cheever AW, Duvall RH, Barral-Netto M. Parasitological and pathological findings in capuchin monkeys Infected with Schistosoma japonicum or Schistosoma mansoni. Am J Trop Med Hyg. 1982;31: 983–987. pmid:7125064

49. Christie JD, Crouse D, Kelada AS, Anis-Ishak E, Smith JH, Kamel IA. Patterns of Schistosoma haematobium egg distribution in the human lower urinary tract. Am J Trop Med Hyg. 1986;35: 759–764. pmid:3089035

50. Kuntz RE, Moore JA, Huang TC. Distribution of egg deposits and gross lesions in nonhuman primates infected with Schistosoma haematobium (Iran). J Med Primatol. 1979;8: 167–178. pmid:116002

51. Smith JH, Kamel IA, Elwi A, Lichtenberg Von F. A quantitative post mortem analysis of urinary schistosomiasis in Egypt. I. Pathology and pathogenesis. Am J Trop Med Hyg. 1974;23: 1054–1071. pmid:4429178

52. Gelfand M, Ross WF. II. The distribution of schistosome ova in the genito-urinary tract in subjects of bilharziasis. Trans R Soc Trop Med Hyg. 1953;47: 218–220. pmid:13077721

53. Charlewood GP, Shippel S, Renton H. Schistosomiasis in gynaecology. J Obstet Gynaecol Br Emp. 1949;56: 367–385. pmid:18132324

54. Gelfand M, Ross MD, Blair DM, Weber MC. Distribution and extent of schistosomiasis in female pelvic organs, with special reference to the genital tract, as determined at autopsy. Am J Trop Med Hyg. 1971;20: 846–849. pmid:5131693

55. Poggensee G, Feldmeier H. Female genital schistosomiasis: facts and hypotheses. Acta Tropica. 2001;79: 193–210. pmid:11412803

56. Gelfand M, Ross CM, Blair DM, Castle WM, Weber MC. Schistosomiasis of the male pelvic organs. Severity of infection as determined by digestion of tissue and histologic methods in 300 cadavers. Am J Trop Med Hyg. 1970;19: 779–784. pmid:5453906

57. Edington GM, Lichtenberg Von F, Nwabuebo I, Taylor JR, Smith JH. Pathologic effects of schistosomiasis in Ibadan, Western State of Nigeria. I. Incidence and intensity of infection; distribution and severity of lesions. Am J Trop Med Hyg. 1970;19: 982–995. pmid:5493057

58. Webbe G, James C. A comparison of two geographical strains of Schistosoma haematobium. J Helminthol. 1971;45: 271–284. pmid:5123704

59. Cheever AW, Mosimann JE, Deb S, Cheever EA, Duvall RH. Natural history of Schistosoma mansoni infection in mice: egg production, egg passage in the feces, and contribution of host and parasite death to changes in worm numbers. Am J Trop Med Hyg. 1994;50: 269–280. pmid:8147486

60. Pittella JE. Neuroschistosomiasis. Brain Pathol. 1997;7: 649–662. pmid:9034571

61. Cheever AW, Macedonia JG, Mosimann JE, Cheever EA. Kinetics of egg production and egg excretion by Schistosoma mansoni and S. japonicum in mice infected with a single pair of worms. Am J Trop Med Hyg. 1994;50: 281–295. pmid:8147487

62. Candido RRF, Morassutti AL, Graeff-Teixeira C, Pierre TGS, Jones MK. Exploring structural and physical properties of Schistosome eggs: potential pathways for novel diagnostics? In: Rollinson D, Sothard JR, editors. Advances in Parasitology Vol. 100, 1st ed. Elsevier Ltd; 2018. pp. 209–237. https://doi.org/10.1016/bs.apar.2018.03.003 pmid:29753339

63. Costain AH, MacDonald AS, Smits HH. Schistosome egg migration: mechanisms, pathogenesis and host immune responses. Front Immunol. 2018;9: 902–16.

64. Schwartz C, Fallon PG. Schistosoma “Eggs-iting” the host: granuloma formation and egg excretion. Front Immunol. 2018;9: 2163–16.

65. Ashton PD, Harrop R, Shah B, Wilson RA. The schistosome egg: development and secretions. Parasitology. 2002;122: 1–11.