Abstract

Duck enteritis virus (DEV) is a member of the Alphaherpesvirinae subfamily. The characteristics of some DEV genes have been reported. However, information regarding the DEV UL47 gene is limited. In this study, we identified the DEV UL47 gene encoding a late structural protein located in the nucleus of infected cells. We further found that two domains of DEV pUL47, amino acids (aa) 40 to 50 and 768 to 777, could function as nuclear localization sequence (NLS) to guide the nuclear localization of pUL47 and nuclear translocation of heterologous proteins, including enhanced green fluorescent protein (EGFP) and beta-galactosidase (β-Gal). Moreover, pUL47 significantly inhibited polyriboinosinic:polyribocytidylic acid [poly(I:C)]-induced interferon beta (IFN-β) production and downregulated interferon-stimulated gene (ISG) expression, such as Mx and oligoadenylate synthetase-like (OASL), by interacting with signal transducer and activator of transcription-1 (STAT1).

Details

Title
Duck enteritis virus pUL47, as a late structural protein localized in the nucleus, mainly depends on residues 40 to 50 and 768 to 777 and inhibits IFN-β signalling by interacting with STAT1
Author
He, Tianqiong; Wang, Mingshu; Cheng, Anchun  VIAFID ORCID Logo  ; Yang, Qiao; Jia, Renyong; Wu, Ying; Huang, Juan; Chen, Shun; Xin-Xin, Zhao; Liu, Mafeng; Zhu, Dekang; Zhang, Shaqiu; Ou, Xuming; Mao, Sai; Gao, Qun; Sun, Di; Wen, XinJian; Tian, Bin; Liu, Yunya; Yu, Yanling; Zhang, Ling; Pan, Leichang; Chen, Xiaoyue
Pages
1-12
Section
Research article
Publication year
2020
Publication date
2020
Publisher
BioMed Central
ISSN
09284249
e-ISSN
12979716
Source type
Scholarly Journal
Language of publication
English
DOI
https://doi.org/10.1186/s13567-020-00859-w
ProQuest document ID
2462237936
Copyright
© 2020. This work is licensed under http://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.