Content area

Abstract

A laminated paper-based analytical device (LPAD) for histidine detection was fabricated from a chromatography filtration paper and laminate films. Histidine recognition was effected by histidyl-tRNA synthetase (HisRS), and its detection was signaled colorimetrically based on the molybdenum blue reaction. The analytical conditions and detectable concentration range of histidine were examined. The method provided selective quantification from 1 to 100 μM histidine. LPAD fabrication is considerably simple, involving only the craft-cutting of the chromatography filtration paper and laminate film, and is cost-effective.

Details

Title
Microfluidic Paper-Based Analytical Device for Histidine Determination
Author
Kugimiya Akimitsu 1   VIAFID ORCID Logo  ; Fujikawa Akane 1 ; Jiang, Xiao 2 ; Hugh, Fan Z 2 ; Nishida Toshikazu 3 ; Kohda Jiro 1 ; Nakano Yasuhisa 1 ; Takano, Yu 1 

1  Hiroshima City University, Department of Biomedical Information Sciences, Graduate School of Information Sciences, Hiroshima, Japan (GRID:grid.443704.0) (ISNI:0000 0001 0706 4814) 
2  University of Florida, Department of Mechanical and Aerospace Engineering, Gainesville, USA (GRID:grid.15276.37) (ISNI:0000 0004 1936 8091) 
3  University of Florida, Department of Electrical and Computer Engineering, Gainesville, USA (GRID:grid.15276.37) (ISNI:0000 0004 1936 8091) 
Pages
812-821
Publication year
2020
Publication date
Nov 2020
Publisher
Springer Nature B.V.
ISSN
02732289
e-ISSN
15590291
Source type
Scholarly Journal
Language of publication
English
DOI
https://doi.org/10.1007/s12010-020-03365-z
ProQuest document ID
2471521094
Copyright
© Springer Science+Business Media, LLC, part of Springer Nature 2020.