Abstract

Objectives

Occupational exposure to trichloroethylene (TCE) induces trichloroethylene hypersensitivity syndrome (TCEHS), which causes hypersensitivity dermatitis and hepatitis. However, whether TCE itself or its two metabolites, trichloroethanol (TCEOH) and trichloroacetic acid (TCA), are involved in TCEHS remains unclear. Therefore, in this study we explored the allergens causing TCEHS and characterized TCEHS‐related liver injury in guinea pigs.

Method

The guinea pig maximization test was performed using TCE, TCEOH, and TCA as candidate allergens. Skin inflammation was scored, and liver function and histopathological changes were evaluated by biochemical tests and hematoxylin and eosin staining, respectively.

Results

The sensitization rates for TCE, TCEOH, and TCA were 90.0%, 50.0%, and 0.0%, respectively. In the TCE and TCEOH experimental groups, the skin showed varying degrees of erythema with eosinophil granulocyte infiltration in the dermis. Additionally, serum alanine aminotransferase and γ‐glutamyl transpeptidase levels increased significantly, and histological analysis revealed focal hepatocellular necrosis with inflammatory cell infiltration in the liver.

Conclusions

TCE is the main cause of allergy and TCEOH is a secondary factor for allergy in guinea pigs. TCE and TCEOH can cause immune‐mediated skin sensitization complicated by focal hepatic necrosis.

Details

Title
Trichloroethylene and trichloroethanol induce skin sensitization with focal hepatic necrosis in guinea pigs
Author
Zhao, Na 1   VIAFID ORCID Logo  ; Song, Xiangrong 2 ; Naito, Hisao 3 ; Li, Hongling 2 ; Huang, Yongshun 1 ; Liu, Lili 4 ; Lu, Fengrong 2 ; Cai, Tingfeng 2 ; Ito, Yuki 5   VIAFID ORCID Logo  ; Kamijima, Michihiro 5 ; Huang, Hanlin 6 ; Nakajima, Tamie 7 ; Wang, Hailan 2 

1  Department of clinical laboratory, Guangdong Province Hospital for Occupational Disease Prevention and Treatment, Guangzhou, China; Guangdong Provincial Key Laboratory of Occupational Disease Prevention and Treatment, Guangdong Province Hospital for Occupational Disease Prevention and Treatment, Guangzhou, China 
2  Institute of Toxicology, Guangdong Province Hospital for Occupational Disease Prevention and Treatment, Guangzhou, China 
3  Department of Public Health, Fujita Health University School of Medicine, Toyoake, Japan 
4  Guangdong Provincial Key Laboratory of Occupational Disease Prevention and Treatment, Guangdong Province Hospital for Occupational Disease Prevention and Treatment, Guangzhou, China 
5  Department of Occupational and Environmental Health, Nagoya City University Graduate School of Medical Sciences, Nagoya, Japan 
6  Guangdong Provincial Key Laboratory of Occupational Disease Prevention and Treatment, Guangdong Province Hospital for Occupational Disease Prevention and Treatment, Guangzhou, China; Department of Scientific Education, Guangdong Provincial Maternal and Child Health Hospital, Guangzhou, China 
7  Department of Life and Health Sciences, Chubu University, Kasugai, Japan 
Section
ORIGINAL ARTICLES
Publication year
2020
Publication date
Jan/Dec 2020
Publisher
Oxford University Press
ISSN
13419145
e-ISSN
13489585
Source type
Scholarly Journal
Language of publication
English
DOI
https://doi.org/10.1002/1348-9585.12142
ProQuest document ID
2472959316
Copyright
© 2020. This work is published under http://creativecommons.org/licenses/by-nc-nd/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.