Abstract

Enterohemorrhagic Escherichia coli (EHEC) induces changes to the intestinal cell cytoskeleton and formation of attaching and effacing lesions, characterized by the effacement of microvilli and then formation of actin pedestals to which the bacteria are tightly attached. Here, we use a Caenorhabditis elegans model of EHEC infection to show that microvillar effacement is mediated by a signalling pathway including mitotic cyclin-dependent kinase 1 (CDK1) and diaphanous-related formin 1 (CYK1). Similar observations are also made using EHEC-infected human intestinal cells in vitro. Our results support the use of C. elegans as a host model for studying attaching and effacing lesions in vivo, and reveal that the CDK1-formin signal axis is necessary for EHEC-induced microvillar effacement.

Enterohemorrhagic Escherichia coli (EHEC) induces formation of attaching and effacing lesions in the intestine. Here, Huang et al. use human intestinal cells and a C. elegans model of infection to show that the process is mediated by a host signaling pathway involving cyclin-dependent kinase CDK1 and formin CYK1.

Details

Title
Host CDK-1 and formin mediate microvillar effacement induced by enterohemorrhagic Escherichia coli
Author
Cheng-Rung, Huang 1 ; Cheng-Ju, Kuo 1   VIAFID ORCID Logo  ; Huang, Chih-Wen 2 ; Yu-Ting, Chen 2 ; Bang-Yu, Liu 2 ; Chung-Ta, Lee 3 ; Po-Lin, Chen 4   VIAFID ORCID Logo  ; Chang Wen-Tsan 1 ; Yun-Wen, Chen 5 ; Lee, Tzer-Min 6 ; Hui-Chen, Hsieh 1 ; Chang-Shi, Chen 1   VIAFID ORCID Logo 

1  National Cheng Kung University, Department of Biochemistry and Molecular Biology, College of Medicine, Tainan, Taiwan (GRID:grid.64523.36) (ISNI:0000 0004 0532 3255); National Cheng Kung University, Institute of Basic Medical Sciences, College of Medicine, Tainan, Taiwan (GRID:grid.64523.36) (ISNI:0000 0004 0532 3255) 
2  National Cheng Kung University, Department of Biochemistry and Molecular Biology, College of Medicine, Tainan, Taiwan (GRID:grid.64523.36) (ISNI:0000 0004 0532 3255) 
3  National Cheng Kung University, Department of Pathology, College of Medicine, Tainan, Taiwan (GRID:grid.64523.36) (ISNI:0000 0004 0532 3255) 
4  National Cheng Kung University, Department of Medicine, College of Medicine, Tainan, Taiwan (GRID:grid.64523.36) (ISNI:0000 0004 0532 3255); National Cheng Kung University, Department of Microbiology and Immunology, College of Medicine, Tainan, Taiwan (GRID:grid.64523.36) (ISNI:0000 0004 0532 3255) 
5  National Cheng Kung University, Institute of Basic Medical Sciences, College of Medicine, Tainan, Taiwan (GRID:grid.64523.36) (ISNI:0000 0004 0532 3255); National Cheng Kung University, Department of Pharmacology, College of Medicine, Tainan, Taiwan (GRID:grid.64523.36) (ISNI:0000 0004 0532 3255) 
6  Kaohsiung Medical University, School of Dentistry, Kaohsiung, Taiwan (GRID:grid.412019.f) (ISNI:0000 0000 9476 5696); National Cheng Kung University, Institute of Oral Medicine, College of Medicine, Tainan, Taiwan (GRID:grid.64523.36) (ISNI:0000 0004 0532 3255) 
Publication year
2021
Publication date
2021
Publisher
Nature Publishing Group
e-ISSN
20411723
Source type
Scholarly Journal
Language of publication
English
DOI
https://doi.org/10.1038/s41467-020-20355-1
ProQuest document ID
2474984631
Copyright
© The Author(s) 2021. This work is published under https://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.