Content area
Abstract
RNA secondary structure is critical to RNA regulation and function. We report a new N3-kethoxal reagent that allows fast and reversible labeling of single-stranded guanine bases in live cells. This N3-kethoxal-based chemistry allows efficient RNA labeling under mild conditions and transcriptome-wide RNA secondary structure mapping.
The authors designed a chemical probe, azido-kethoxal, to specifically label guanosine in single-strand RNAs in live cells that could be used to determine transcriptome-wide RNA secondary structures.
Details
Title
Keth-seq for transcriptome-wide RNA structure mapping
Author
Weng Xiaocheng 1 ; Gong Jing 2 ; Chen, Yi 3 ; Wu, Tong 4 ; Wang, Fang 5 ; Yang Shixi 3 ; Yuan Yushu 3 ; Luo Guanzheng 4
; Chen, Kai 4 ; Hu, Lulu 4 ; Ma, Honghui 4 ; Wang Pingluan 4 ; Zhang Qiangfeng Cliff 2
; Zhou, Xiang 3 ; He, Chuan 4
1 Institute for Biophysical Dynamics, Howard Hughes Medical Institute, The University of Chicago, Department of Chemistry, Department of Biochemistry and Molecular Biology, Chicago, USA (GRID:grid.170205.1) (ISNI:0000 0004 1936 7822); Wuhan University, College of Chemistry and Molecular Sciences, Key Laboratory of Biomedical Polymers of Ministry of Education, Wuhan, China (GRID:grid.49470.3e) (ISNI:0000 0001 2331 6153)
2 Tsinghua University, MOE Key Laboratory of Bioinformatics, Beijing Advanced Innovation Center for Structural Biology, Center for Synthetic and Systems Biology, Tsinghua-Peking Joint Center for Life Sciences, School of Life Sciences, Beijing, China (GRID:grid.12527.33) (ISNI:0000 0001 0662 3178)
3 Wuhan University, College of Chemistry and Molecular Sciences, Key Laboratory of Biomedical Polymers of Ministry of Education, Wuhan, China (GRID:grid.49470.3e) (ISNI:0000 0001 2331 6153)
4 Institute for Biophysical Dynamics, Howard Hughes Medical Institute, The University of Chicago, Department of Chemistry, Department of Biochemistry and Molecular Biology, Chicago, USA (GRID:grid.170205.1) (ISNI:0000 0004 1936 7822)
5 Institute for Biophysical Dynamics, Howard Hughes Medical Institute, The University of Chicago, Department of Chemistry, Department of Biochemistry and Molecular Biology, Chicago, USA (GRID:grid.170205.1) (ISNI:0000 0004 1936 7822); Wuhan University School of Pharmaceutical Sciences, Wuhan, China (GRID:grid.49470.3e) (ISNI:0000 0001 2331 6153)
; Chen, Kai 4 ; Hu, Lulu 4 ; Ma, Honghui 4 ; Wang Pingluan 4 ; Zhang Qiangfeng Cliff 2
; Zhou, Xiang 3 ; He, Chuan 4
1 Institute for Biophysical Dynamics, Howard Hughes Medical Institute, The University of Chicago, Department of Chemistry, Department of Biochemistry and Molecular Biology, Chicago, USA (GRID:grid.170205.1) (ISNI:0000 0004 1936 7822); Wuhan University, College of Chemistry and Molecular Sciences, Key Laboratory of Biomedical Polymers of Ministry of Education, Wuhan, China (GRID:grid.49470.3e) (ISNI:0000 0001 2331 6153)
2 Tsinghua University, MOE Key Laboratory of Bioinformatics, Beijing Advanced Innovation Center for Structural Biology, Center for Synthetic and Systems Biology, Tsinghua-Peking Joint Center for Life Sciences, School of Life Sciences, Beijing, China (GRID:grid.12527.33) (ISNI:0000 0001 0662 3178)
3 Wuhan University, College of Chemistry and Molecular Sciences, Key Laboratory of Biomedical Polymers of Ministry of Education, Wuhan, China (GRID:grid.49470.3e) (ISNI:0000 0001 2331 6153)
4 Institute for Biophysical Dynamics, Howard Hughes Medical Institute, The University of Chicago, Department of Chemistry, Department of Biochemistry and Molecular Biology, Chicago, USA (GRID:grid.170205.1) (ISNI:0000 0004 1936 7822)
5 Institute for Biophysical Dynamics, Howard Hughes Medical Institute, The University of Chicago, Department of Chemistry, Department of Biochemistry and Molecular Biology, Chicago, USA (GRID:grid.170205.1) (ISNI:0000 0004 1936 7822); Wuhan University School of Pharmaceutical Sciences, Wuhan, China (GRID:grid.49470.3e) (ISNI:0000 0001 2331 6153)
Pages
489-492
Publication year
2020
Publication date
May 2020
ISSN
15524450
e-ISSN
15524469
Source type
Scholarly Journal
Language of publication
English
ProQuest document ID
2476741663
Copyright
© The Author(s), under exclusive licence to Springer Nature America, Inc. 2020.