Evaluation of Fluorescent Cu2+ Probes: Instant Sensing, Cell Permeable Recognition and Quantitative Detection

Abstract

By incorporating a rhodamine spirolactam structure as the recognition site for Cu²⁺, two novel probes were synthesized through a connection of rhodamine 6G acylhydrazine and 5-formyl-6-hydroxyl-4-methylcoumarin/2,4-dihydroxybenzaldehyde. In the recognition process of probes towards Cu²⁺, the spirolactam ring exhibited opening and closing, accompanying an instant and specific change in fluorescence and in color, which could also achieve a naked-eye and semiquantitative recognition of aqueous Cu²⁺ besides the fluorescent Cu²⁺ detection method. Fluorescent analyses and ECV304 cell imaging further revealed the probes’ good optical stability, instant response, low toxicity, and membrane permeability, which offers future possibilities for the probes’ instant detection and the real-time tracking of Cu²⁺ in biological systems.

1. Introduction

As an abundantly existing element with the highest concentration occurring in the brain [1], copper [2,3] is an active participant in the human body and its abnormal changes have been proven to be connected with a number of neurodegenerative diseases [4,5]. On the one hand, there are commonly coexisting oxidation states of cuprous and copper ions in the biological systems, on the other hand, there are a variety of complex changes of copper, including the distribution, storage, and transportation, thus, if out of order [6], the dynamic changes of copper would induce abnormal accumulation or uncontrolled oxidation-reduction reactions [7], then disrupt the natural balance or trigger chain reactions, finally result in neurodegenerative diseases like Alzheimer’s and Parkinson’s Diseases [8]. In order to better understand the dynamic changes of copper, increasing copper-detecting techniques, especially those that could efficiently distinguish cuprous ions from copper ones without sample damages, need to be developed.

With numerous Cu²⁺-detecting methods [9,10] having been developed, the fluorescent probe method has been receiving much attention for the probes’ characteristics, such as tangible fluorescent signals [11] and instant responses to the target [12,13]. Especially, Cu²⁺ probes of rhodamine spirolactam-type, outstand for their remarkable copper ions assessment and specific color changes accompanied the recognition process. From the pioneering studies of rhodamine Cu²⁺ probes by A. Czarnik [14], to D. Wu [15] and P. Li [16], until recent research works by C. Liu [17] and M. Tian [18], increasing efforts were made to solve the remained problems in practical Cu²⁺ detections, such as enhancing the probes’ sensitivity and selectivity [19] towards Cu²⁺ in complex environments or biological systems, enforcing the probes’ long-wavelength excitation and emission characteristics [20] to avoid biological interferences, increasing the probes’ solubilities [15] and affinities [21], and so on.

Accordingly, two novel Cu²⁺ probes were synthesized through a connection of rhodamine 6G moiety to a coumarin derivative (to obtain probe J6) and to a salicylaldehyde derivative (to obtain J7), respectively (Figure 1). The probes demonstrated specific responses to Cu²⁺ over other metals (Cu⁺ included) with remarkable spectral changes in both aqueous solutions and living cells. Apart from the highly qualitative Cu²⁺ detection, the synthesized two probes possessed good quantitative relationship and steady combination with Cu²⁺ in a relatively wide concentration range, not only at different pH values, but in time-lapse environments. Their good qualitative and quantitative relationships offer the probes a practical value for instant detection and time-lapse tracking of intracellular Cu²⁺ in biological systems. Additionally, semiquantitative Cu²⁺ test paper was prepared based on the probes’ naked-eye recognition characteristics, giving us a quick and convenient Cu²⁺ colorimetric method.

2. Results 2.1. Structure Characterization By introducing coumarin and salicylaldehyde derivatives to a rhodamine 6G moiety, two novel fluorescent probes J6 and J7 for Cu²⁺ were synthesized. The structures of J6 and J7 were characterized by IR, ¹H NMR and MS. The calculation and measurement results of MS were all matched, ¹H NMR chemical shifts were in accordance with the target structures, and the characteristic absorbance peaks of newly synthesized functional groups C=O and C=N, both located at the spirolactam ring, were at the reasonable ranges of ~1700 and 1600~1620 cm⁻¹ in IR spectra. 2.2. Metal Ion Selectivity

In biological systems, the complex coexistence of multiple ions is making the task of a specific detection on the target ion much more difficult. And high selectivity has been therefore becoming a very important evaluation parameter concerning the probes’ performance. By incorporating the spirolactam ring with an opening and closing transformation, the synthesized rhodamine probes J6 and J7 showed highly critical responses towards Cu²⁺. When excited by the wavelength of 470 nm, neither probe J6 itself nor the coexistence of J6 and other ions except Cu²⁺ displayed obvious fluorescence emission at 529 nm, indicating a predominantly closed spirolactam form in J6. Otherwise, the addition of Cu²⁺ could immediately trigger a significant fluorescence intensity enhancement at 529 nm (Figure 2a), only with a relatively low interference coming from Fe³⁺, which suggested the opening of initially closed spirolactam ring in the recognition process of J6 to Cu²⁺. A similar phenomenon was observed in J7 and the fluorescence increase of J7-Cu²⁺ emerged at 560 nm when excited by 507 nm (Figure 2b).

In the presence of other metal ions, experiments on ions competition were conducted by adding Cu²⁺ to the solution of probe J6 (J6: 10 μmol L⁻¹, metal ions: 10 μmol L⁻¹) at an excited wavelength of 470 nm. The spectral enhancement induced by Cu²⁺ at 529 nm, evidently, was almost not affected by the presence of other metal ions (Figure 3a). The ions competition tests indicated that probe J6 was highly sensitive towards Cu²⁺ and provide a future use of J6 for Cu²⁺ detection in complex environments. Similar phenomena were observed at 560 nm for J7 when excited by 507 nm (Figure 3b).

2.3. Visual Detection of Copper Ions

During the detections of Cu²⁺ by J6/J7, a significant color change, accompanying the fluorescence enhancement, emerged immediately upon the adding of Cu²⁺ to the probe, with low interferences of Fe³⁺. Instead, other metal ions’ addition did not induce any color changes (Figure 4).

Before and after Cu²⁺ addition, the J6/J7 solution exhibited an obvious and rhodamine-characteristic color change from colorless J6/J7 to the rhodamine red with an open spirolactam ring in J6/J7-Cu²⁺, which implied that J6/J7 showed a high selectivity for Cu²⁺ and could serve as “naked-eye” probes. 2.4. Quantitative Detection of Copper Ions

To analyze the quantitative relationship of J6/J7 and Cu²⁺, fluorescence titration was performed. As shown in Figure 5a of J6 and Figure 5b of J7, probe J6 itself with a predominant spirolactam form displayed nearly no fluorescence at 529 nm, while addition of Cu²⁺ immediately induced opening of the initially closed rhodamine-spirolactam ring, accompanied by a significant fluorescence enhancement at 529 nm. To furtherly analyze the 529 nm fluorescence intensity of J6-Cu²⁺ system, a linear relationship between fluorescence intensity and concentration of Cu²⁺ was obtained, indicating a highly quantitative detection of Cu²⁺ by J6 in the concentration range of 0~10 μmol L⁻¹ Cu²⁺ (Figure 5a, inset). The linear response of fluorescence emission intensity at 529 nm towards [Cu²⁺] in the range of 0~10 μmol L⁻¹ could be expressed by the following Stern-Volmer Equation [22,23] through an analysis on the relationship of F/F₀ (F: fluorescence intensity of the probe-Cu²⁺ system, F₀: fluorescence intensity of the probe) versus [Cu²⁺]: y = 0.6104x+1.0427 (R² = 0.9668). The association constant was K_a = 1.64 × 10⁶ L mol⁻¹, and the corresponding detection limit of Cu²⁺ by J6 was calculated to be 0.592 μmol L⁻¹.

A stoichiometry calculation was performed to better understand the probes’ sensitivity, indicating a 1:1 stoichiometry between J6 and Cu²⁺ was adopted, as shown in Figure 6a.

As for J7, the linear relationship at 560 nm towards [Cu²⁺] in the range of 0~6 μmol L⁻¹ (Figure 5b, inset) based on Stern-Volmer Equation could be expressed as y = 0.2762x + 0.9762 (R² = 0.9611), with a Cu²⁺ detection limit of 0.977 μmol L⁻¹ and the association constant K_a as 3.62 × 10⁶ L mol⁻¹, also demonstrating 1:1 association stoichiometry between J7 and Cu²⁺ (Figure 6b).

A semiquantitative test paper was prepared for a compatible detection of Cu²⁺, the characteristic rhodamine red of an opening spirolactam ring exhibited a positive correlation with [Cu²⁺] through gradient color changes (Figure 7), which could be distinguished by the naked eye, affording a quick and convenient Cu²⁺ semiquantitative colorimetric method.

2.5. Influences of pH and Time on the Detection of Copper Ions

Considering the complex environment in biological applications of the probes, the fluorescence stability at physiological pH [24] was examined. At a varied pH region of 5.4 to 7.4, fluorescence intensity of both the J6/J7 and J6/J7-Cu²⁺ systems kept steady (Figure S1), suggesting a relatively wide pH range from 5.4 to 7.4 appropriate for J6/J7-Cu²⁺ combination system.

As for time influence on the fluorescence intensity of probe-Cu²⁺ system, the J6/J7-Cu²⁺ remained steady for hours (Figure S2), indicating a possible time-lapse imaging application apart from an instant fluorescence imaging.

2.6. MTT Assay

To evaluate the potential imaging and diagnostic applications [15] of the two probes J6 and J7, MTT assay was performed in ECV304 cells with a concentration gradient of 125, 250, 500, 1000, 2000 and 4000 μg L⁻¹ for each probe. The cytotoxicity of J6 and J7 in ECV304 cells at 24 h were compiled in Table S1 and depicted in Figure 8, indicating that there was no significant toxic effect of J6 and J7 on ECV304 cells when incubated for 24 h. Even when the concentration of J6/J7 reached the highest 4000 μg L⁻¹ in the assay, the cytotoxicity of ECV304 cells remained below 30%, indicating a relatively low cytotoxicity of J6/J7 in the range of 125~4000 μg L⁻¹, which satisfies the cytotoxicity-concentration demands of μmol L⁻¹ Cu²⁺ distributions in most biological systems.

2.7. Recognition Mechanism

Besides a structural transformation of the spirolactam ring from initially closed to later opening in the recognition process of probe-Cu²⁺, fluorescence increase at the target emission wavelength could also be attributed to a possible structural cavity [15,25] to capture Cu²⁺ (Figure 9) and electron effect [26].

In J6/J7, the two oxygen and one nitrogen atoms located around the spirolactam ring, with higher nucleophilic activity towards Cu²⁺, probably act as the recognition sites to capture target Cu²⁺. When the spirolactam ring opens and thus the three atoms form an appropriate cavity to hold Cu²⁺, then the unbonding electron pairs of the two oxygen and one nitrogen atoms will partly be provided to fulfill the empty electron orbits of Cu²⁺ through intramolecular forces [27,28], though weaker than coordination bonds, an obvious electron transfer [29] will then be established and two chelating five- and six-membered rings formed, which contributes to a better planarity and rigid of the J6/J7-Cu²⁺ system. And stronger fluorescence intensity of the J6/J7-Cu²⁺ system accompanied with the extended conjugated chain (the extended conjugation chain was labelled in blue in Figure 9) and better molecular planarity.

Unlike the reaction-based irreversible chemosensors for copper (II), probes J6/J7 bind reversibly to Cu²⁺ due to their multidentate chelation model [30] in the recognition mechanism and could be better applied to Cu²⁺ detections in mild-reaction needed biological environments.

2.8. Bioimaging

After an initial incubation of ECV304 and J6/J7 (Figure 10a), 10 μmol L⁻¹ Cu²⁺ was added in, incubated for another 2 h at 37 °C, and red fluorescence was emitted from the intracellular area (Figure 10b) in comparison with Figure 10a, demonstrating the recognition process of added Cu²⁺ by J6/J7 accomplished almost instantly. The overlay of fluorescence image (Figure 10b) and bright-field transmission image (Figure 10c) revealed that the fluorescence signals were from the cytosol [31,32] (Figure 10d), which indicated the good cell permeability of J6/J7 and the future applications of J6/J7 for dynamic imaging and tracking of Cu²⁺ in living cells.

3. Discussion Novel rhodamine-based Cu²⁺ probes J6 and J7 were synthesized, with their structures characterized by IR, MS, and ¹H NMR, and biological applications analyzed by FS, MTT, and bioimaging. J6 and J7 demonstrated high selectivity to Cu²⁺ over other metal ions and could be used for highly specific recognition of Cu²⁺ in ions coexisting environment. Accompanying the recognition process was a significant color change, which enabled the “naked-eye” detection of aqueous Cu²⁺ and was prepared as a compatible and semiquantitative Cu²⁺ test paper. Further fluorescence titration and stoichiometry revealed the detection limits of Cu²⁺ by J6/J7 to be 0.592 and 0.977 μmol L⁻¹, although not as good as the 10⁻⁸ mol L⁻¹ concentration limit seen in some commercial Cu²⁺ probes, the LOD value could still satisfy the demands of μmol L⁻¹ Cu²⁺ distributions in most biological systems. Low cytotoxicity and instant signaling in fluorescence imaging of Cu²⁺ in living cells ECV304 suggested the probe’s future applications as an instant Cu²⁺ detection method in clinical diagnosis and a dynamic tracking tool for Cu²⁺ in biological systems. 4. Materials and Methods 4.1. Materials and Instrumentation All the reagents and solvents used for synthesis were analytically pure and used without further treatment unless otherwise noted. The ECV304 cells were purchased from KeyGen BioTECH (Nanjing, China). The reaction process was monitored by thin-layer chromatography (TLC) on silica gel GF254. The products were purified by column chromatography on Merck silica gel (250–400 mesh ASTM). A Tris-HCl buffer solution (pH 7.4) was prepared using 0.1 mol L⁻¹ HCl and proper amount of a 0.1 mol L⁻¹ Tris stock solution (Sinopharm Chemical Reagent Company, Shanghai, China). Double distilled water was used throughout the process of solution preparing and spectroscopic testing. Metal ion solutions were prepared from their nitrate and chloride salts, KCl, NaCl, AgNO₃, Ca(NO₃)₂·4H₂O, Mg(NO₃)₂·6H₂O, BaCl₂, NiCl₂, PbCl₂, MnCl₂, CoCl₂, Zn(NO₃)₂·6H₂O, Cd(NO₃)₂·2H₂O, SnCl₂, HgCl₂, AlCl₃, Cr(NO₃)₃·9H₂O, FeCl₂, Fe(NO₃)₃·9H₂O, CuCl, and Cu(NO₃)₂·3H₂O, respectively. Fourier Transform-Infrared (FT-IR) spectra were recorded with KBr pellets on a Bruker EQUINOX-55 FT-IR spectrometer (Karlsruhe, Germany). Elemental Analyses (EA) were recorded on an Elementar varioEL III analyzer. ¹H Nuclear Magnetic Resonance (¹H NMR) spectra were recorded on a Varian INOVA-400 spectrometer (Palo Alto, CA, USA) at 400 MHz and chemical shifts were reported relative to the internal standard tetramethylsilane (TMS). Fluorescence spectra were recorded on a HITACHI F-4500 fluorescence spectrophotometer (Tokyo, Japan). Mass Spectrometry (MS) analyses were performed using an Agilent 1260-6460A triple quadrupole liquid chromatograph-mass spectrometer (Santa Clara, CA, USA). The cytotoxicity results were analyzed with the SoftMax Pro Software (version 2.2.1) (San Jose, CA, USA) in a Molecular Devices SpectraMax 190 microplate reader (San Jose, CA, USA). The cell-imaging experiments were performed using an OLYMPUS U-LH 100HG IX73 fluorescence microscope. 4.2. Synthesis and Characterization of Probes J6/J7

Target probes J6 and J7 were synthesized in a route shown in Scheme 1. The structures of J6 and J7 were characterized by IR, ¹H NMR, and MS.

4.2.1. 6-Hydroxyl-4-Methylcoumarin (Scheme 1a)

With the temperature controlled between 0 and 5 °C in an ice bath, p-benzenediol (5.0 g, 0.045 mol) and ethyl acetoacetate (15 mL, 0.12 mol) were first added to concentrated sulfuric acid (25 mL), then the mixture was kept between 0 and 5 °C and stirred for 24 h, poured into iced water (100 mL), filtered, and subsequently purified by column chromatography (silica gel, V (EtOAc): V (hexane) = 1: 2 as the eluent) to obtain 6-hydroxyl-4-methylcoumarin (a).

6-Hydroxyl-4-methylcoumarin: yellow solid, 5.2 g, yield 75%. IR (KBr pellet, υ/cm⁻¹): 3342 (υ_–OH); 1691 (υ_C=O); 1580 (υ_C=C); 1443 (σ_–OH); 1232 (υ_C–O–C) (Figure S3). EA: C 68.17%, H 4.55% (calcd. for C₁₀H₈O₃: C 68.18%, H 4.58%).

4.2.2. 5-Formyl-6-Hydroxyl-4-Methylcoumarin (Scheme 1b)

With the temperature controlled between 0 and 5 °C in an ice bath, 6-hydroxyl-4-methylcoumarin (1.0 g, 5.0 mmol) and hexamethylenetetramine (2.0 g, 10 mmol) were added to trifluoroacetic acid (10 mL), the mixture was then refluxed at 70 °C for 10 h, monitored by TLC, cooled down, concentrated in vacuo, poured into iced water (50 mL), filtered, and subsequently purified by column chromatography (silica gel, V (EtOAc):V (hexane) = 1:2 as the eluent) to afford 5-formyl-6-hydroxyl-4-methylcoumarin (b).

5-Formyl-6-hydroxyl-4-methylcoumarin: yellow powder, 0.82 g, yield 63%. IR (KBr pellet, υ/cm⁻¹): 3423 (υ_–OH); 2800, 2700 (υ_C–H); 1606 (υ_C=C); 1367 (σ_–OH) (Figure S4). EA: C 64.74%, H 3.97% (calcd. for C₁₁H₈O₄: C 64.71%, H 3.95%).

4.2.3. Rhodamine 6G Acylhydrazine (Scheme 1c)

At r. t., 98% hydrazine hydrate (3.5 mL, 0.069 mol) was added to a 20 mL ethanol solution of rhodamine 6G (3.0 g, 7.2 mmol); after that, the reaction mixture was refluxed at 80 °C for 3 h, monitored by TLC, then cooled down, filtered and washed with anhydrous ethanol, affording rhodamine 6G acylhydrazine (c).

Rhodamine 6G acylhydrazine: pink solid, 0.76 g, yield 75%. IR (KBr pellet, υ/cm⁻¹): 3427 (υ_–NH2); 1688 (υ_C=O); 2926 (υ_C–H), 1421 (σ_C–H); 1623, 1516 (υ_C=C) (Figure S5). EA: C 72.89%, H 6.57%, N 13.08% (calcd. for C₂₆H₂₈N₄O₂: C 72.87%, H 6.59%, N 13.07%).

4.2.4. Probe J6

The synthesized rhodamine 6G acylhydrazine (0.17 g, 0.40 mmol) and 5-formyl-6-hydroxyl-4-methylcoumarin (0.10 g, 0.50 mmol) in previous steps were dissolved in anhydrous methanol (20 mL) and then refluxed at 65 °C for 1.5 h, monitored by TLC, cooled down, filtered, washed with anhydrous methanol, concentrated in vacuo, and subsequently purified by column chromatography (silica gel, V (EtOAc):V (hexane) = 1:1 as the eluent) to afford J6.

J6: yellow solid, 0.13 g, yield 50%. IR (KBr pellet, υ/cm⁻¹): 3432 (υ_–OH); 1726 (υ_C=O); 1621 (υ_C=N); 1516 (υ_C=C); 1422 (σ_–OH); 1216 (υ_C–O–C) (Figure S6). ¹H-NMR (400 MHz, CDCl₃) δ: 10.89 (s, 2H, 2×–NH–), 8.93 (s, 1H, –OH), 8.06~8.01 (m, 2H, Ar-H), 7.57~7.48 (m, 4H, Ar-H), 7.19~7.14 (m, 2H, Ar-H), 7.03 (s, 2H, Ar-H), 7.01 (s, 2H, 2×=CH–), 2.02 (s, 4H, 2×–CH₂–), 1.92 (s, 15H, 5×–CH₃) (Figure S7). MS: m/z = 614.29 (calcd. for C₃₇H₃₄N₄O₅: 614.25) (Figure S8).

4.2.5. Probe J7

Rhodamine 6G acylhydrazine (0.28 g, 0.60 mmol) and 2,4-dihydroxybenzaldehyde (0.12 g, 0.80 mmol) were initially dissolved in anhydrous methanol (20 mL) and then refluxed at 65 °C for 2 h, monitored by TLC, cooled down, filtered, washed with anhydrous methanol, concentrated in vacuo, and subsequently purified by column chromatography (silica gel, V (EtOAc):V (hexane) = 1:1 as the eluent) to afford J7.

J7: pink solid, 0.20 g, yield 47%. IR (KBr pellet, υ/cm⁻¹): 3427, 3404 (υ_–OH); 1673 (υ_C=O); 1624 (υ_C=N); 1518 (υ_C=C); 1423 (σ_OH); 1222 (υ_C–O–C) (Figure S9). ¹H-NMR (400 MHz, CDCl₃) δ: 11.07 (s, 2H, 2×–NH–), 9.09 (s, 2H, 2×–OH), 8.00 (dd, J = 5.8, 2.6 Hz, 3H, Ar-H), 7.54~7.48 (m, 4H, Ar-H), 7.13 (dd, J = 5.7, 2.6 Hz, 3H, Ar-H), 6.95 (s, 1H, Ar-H), 6.93 (s, 1H, =CH–), 2.03 (s, 4H, 2×–CH₂–), 1.93 (s, 12H, 4×–CH₃) (Figure S10). MS: m/z = 548.29 (calcd. for C₃₃H₃₂N₄O₄: 548.24) (Figure S11).

4.3. Spectroscopic Analysis

Stock solutions (200 μmol L⁻¹) of probe J6/J7, K⁺, Na⁺, Ag⁺, Ca²⁺, Mg²⁺, Ba²⁺, Ni²⁺, Pb²⁺, Mn²⁺, Co²⁺, Zn²⁺, Cd²⁺, Sn²⁺, Hg²⁺, Al³⁺, Cr³⁺, Fe²⁺, Fe³⁺, Cu⁺ and Cu²⁺ were prepared in a mixed solvent system (V (EtOH):V (H₂O) = 5:5, Tris-HCl pH = 7.4) [33]. When used for fluorescence tests, the stock solutions were usually diluted to 10 μmol L⁻¹ with mixed EtOH-H₂O solvent (5:5, V/V, Tris-HCl pH = 7.4) unless otherwise noted. All the spectroscopic measurements were performed at least in triplicate and averaged.

4.4. Cytotoxicity

The cytotoxicity of J6 and J7 in ECV304 cells was analyzed by Methyl Thiazolyl Tetrazolium (MTT) assay [34]. ECV304 cells were cultured in Dulbecco’s Modified Eagle Medium (DMEM) with 10% Fetal Bovine Serum (FBS) in a humidified atmosphere with 5% CO₂ at 37 °C. All cells in the exponential phase of growth were used in the experiments. After digestion with a 0.25% trypsin solution, the cells were seeded in 96-well cell culture clusters with 200 μL per well at a density of 2.5 × 10⁴ cells/mL for 24 h. The probes J6/J7 (100 mmol L⁻¹ in DMSO) were then added to the 96-well plate to achieve a concentration gradient of 125, 250, 500, 1000, 2000, and 4000 μg L⁻¹, and then incubated for another 24 h. Subsequently, the medium was removed, the cells were washed gently with Phosphate Buffered Saline (PBS) three times and incubated with 5.0 mg mL⁻¹ MTT solution at 37 °C for 4 h. After that, the cells were washed gently with PBS three times, 150 μL DMSO was added to each well to dissolve the resulting crystals. The optical density was measured at 490 nm on a microplate spectrophotometer. All of the tests were conducted in triplicate. Data were expressed as mean ± standard deviation (SD).

4.5. Fluorescent Imaging in Living Cells

ECV304 cells were dispensed and cultured for 2 h until they plated on glass-bottomed dishes. The medium was then removed, and the cells were washed with DMEM and incubated for 30 min with 10 μmol L⁻¹ of the probes at 37 °C, then washed three times with PBS and imaged [35]. After that, the cells were supplemented with 10 μmol L⁻¹ of Cu²⁺ in the growth medium for another 30 min at 37 °C, washed three times with PBS, and imaged.

Supplementary Materials

The following are available online, Figure S1: Influence of pH on J6/J7 (8 μmol L-1) in the absence and presence of Cu2+ (8 μmol L-1), Figure S2: Influence of time on J6/J7-copper ions system (probe: 8 μmol L-1, Cu2+: 8 μmol L-1), Figure S3: IR of 6-hydroxyl-4-methylcoumarin (a), Figure S4: IR of 5-formyl-6-hydroxyl-4-methylcoumarin (b), Figure S5: IR of rhodamine 6G acylhydrazine (c), Figure S6: IR of probe J6, Figure S7: 1H NMR of probe J6, Figure S8: MS of probe J6, Figure S9: IR of probe J7, Figure S10: 1H NMR of probe J7, Figure S11: MS of probe J7, Table S1: Cytotoxicity of probes J6 and J7 in ECV304 at 24 h.

Author Contributions

Conceptualization, H.H. and Z.C.; methodology, H.H., Z.C. and L.Z.; software, Z.C.; validation, H.H. and Z.C.; formal analysis, L.Z. and X.Z.; investigation, H.H. and Z.C.; resources, H.H. and Z.C.; data curation, L.Z. and X.Z.; writing-original draft preparation, H.H.; writing-review and editing, Z.C.; visualization, Z.C.; supervision, Z.C. and X.Z.; project administration, Z.C.; funding acquisition, H.H., X.Z. and Z.C. All authors have read and agreed to the published version of the manuscript.

Funding

This research was funded by Shaanxi Natural Science Basic Research Project (2019JQ-068), Natural Science Foundation of Shaanxi Provincial Department of Education (20JK0902), Innovative Talents Promotion Plan in Shaanxi Province (2019KJXX-057), the Fund for Young Outstanding Talents in Universities of Shaanxi Province (05041904), Doctor Program of Xi'an Medical University (2020DOC32).

Data Availability Statement

The data presented in this study are available in the article and supplementary material.

Conflicts of Interest

The authors declare no conflict of interest.

Sample Availability

Samples of the compounds are available from the authors.

References

1. Prohaska, J.R. Functions of trace elements in brain metabolism. Physiol. Rev. 1987, 67, 858-901.

2. Stockel, J.; Safar, J.; Wallace, A.C.; Cohen, F.E.; Prusiner, S.B. Prion protein selectively binds copper(II) ions. Biochemistry 1998, 37, 7185-7193.

3. Schlief, M.L.; Gitlin, J.D. Copper homeostasis in the CNS: A novel link between the NMDA receptor and copper homeostasis in the hippocampus. Mol. Neurobiol. 2006, 33, 81-90.

4. Mezzaroba, L.; Alfieri, D.F.; Colado Simao, A.N.; Vissoci Reiche, E.M. The role of zinc, copper, manganese and iron in neurodegenerative diseases. Neurotoxicology 2019, 74, 230-241.

5. Lavado, L.K.; Zhang, M.H.; Patel, K.; Khan, S.; Patel, U.K. Biometals as potential predictors of the neurodegenerative decline in Alzheimer's Disease. Cureus 2019, 11, 5573-5583.

6. Strausak, D.; Mercer, J.F.B.; Dieter, H.H.; Stremmel, W.; Multhaup, G. Copper in disorders with neurological symptoms: Alzheimer's, Menkes, and Wilson diseases. Brain Res. Bull. 2001, 55, 175-185.

7. Watts, R.W.E. Inside story. Nature 1989, 342, 868-869.

8. Gaggelli, E.; Kozlowski, H.; Valensin, D.; Valensin, G. Copper homeostasis and neurodegenerative disorders (Alzheimer's, prion, and Parkinson's diseases and amyotrophic lateral sclerosis). Chem. Rev. 2006, 106, 1995-2044.

9. Yang, L.; McRae, R.; Henary, M.M.; Patel, R.; Lai, B.; Vogt, S.; Fahrni, C.J. Imaging of the intracellular topography of copper with a fluorescent sensor and by synchrotron x-ray fluorescence microscopy. Proc. Natl. Acad. Sci. USA 2005, 102, 11179-11184.

10. Viguier, R.F.; Hulme, A.N. A sensitized europium complex generated by micromolar concentrations of copper(I): Toward the detection of copper(I) in biology. J. Am. Chem. Soc. 2006, 128, 11370-11371.

11. Jun, Y.W.; Cho, S.W.; Jung, J.; Huh, Y.; Kim, Y.; Kim, D.; Ahn, K.H. Frontiers in probing Alzheimer's Disease biomarkers with fluorescent small molecules. ACS Cent. Sci. 2019, 5, 209-217.

12. Lee, M.H.; Kim, J.S.; Sessler, J.L. Small molecule-based ratiometric fluorescence probes for cations, anions, and biomolecules. Chem. Soc. Rev. 2015, 44, 4185-4191.

13. Zeng, L.; Miller, E.W.; Pralle, A.; Isacoff, E.Y.; Chang, C.J. A selective turn-on fluorescent sensor for imaging copper in living cells. J. Am. Chem. Soc. 2006, 128, 10-11.

14. Dujols, V.; Ford, F.; Czarnik, A.W. A long-wavelength fluorescent chemodosimeter selective for Cu(II) ion in water. J. Am. Chem. Soc. 1997, 119, 7386-7387.

15. Wu, D.; Huang, W.; Duan, C.; Lin, Z.; Meng, Q. Highly sensitive fluorescent probe for selective detection of Hg2+ in DMF aqueous media. Inorg. Chem. 2007, 46, 1538-1540.

16. Li, P.; Duan, X.; Chen, Z.; Liu, Y.; Xie, T.; Fang, L.; Li, X.; Yin, M.; Tang, B. A near-infrared fluorescent probe for detecting copper(II) with high selectivity and sensitivity and its biological imaging applications. Chem. Commun. 2011, 47, 7755-7757.

17. Liu, C.; Jiao, X.; He, S.; Zhao, L.; Zeng, X. A highly selective and sensitive fluorescent probe for Cu2+ based on a novel naphthalimide-rhodamine platform and its application in live cell imaging. Org. Biomol. Chem. 2017, 15, 3947-3954.

18. Tian, M.; He, H.; Wang, B.B.; Wang, X.; Liu, Y.; Jiang, F.L. A reaction-based turn-on fluorescent sensor for the detection of Cu(II) with excellent sensitivity and selectivity: Synthesis, DFT calculations, kinetics and application in real water samples. Dyes. Pigm. 2019, 165, 383-390.

19. Ning, J.; Wang, W.; Ge, G.; Chu, P.; Long, F.; Yang, Y.; Peng, Y.; Feng, L.; Ma, X.; James, T.D. Target enzyme-activated two-photon fluorescent probes: A case study of CYP3A4 using a two-dimensional design strategy. Angew. Chem. Int. Ed. Engl. 2019, 58, 9959-9963.

20. Ning, J.; Liu, T.; Dong, P.; Wang, W.; Ge, G.; Wang, B.; Yu, Z.; Shi, L.; Tian, X.; Huo, X.; et al. Molecular design strategy to construct the near-infrared fluorescent probe for selectively sensing human cytochrome P450 2J2. J. Am. Chem. Soc. 2019, 141, 1126-1134.

21. Feng, L.; Yan, Q.; Zhang, B.; Tian, X.; Wang, C.; Yu, Z.; Cui, J.; Guo, D.; Ma, X.; James, T.D. Ratiometric fluorescent probe for sensing Streptococcus mutans glucosyltransferase, a key factor in the formation of dental caries. Chem. Commun. 2019, 55, 3548-3551.

22. Kuijpers, K.P.L.; Bottecchia, C.; Cambié, D.; Drummen, K.; König, N.J.; Noël, T. A fully automated continuous-flow platform for fluorescence quenching studies and Stern-Volmer analysis. Angew. Chem. Int. Ed. Engl. 2018, 57, 11278-11282.

23. Zhu, M.; Yuan, M.; Liu, X.; Xu, J.; Lv, J.; Huang, C.; Liu, H.; Li, Y.; Wang, S.; Zhu, D. Visible near-infrared chemosensor for mercury ion. Org. Lett. 2008, 10, 1481-1484.

24. Zhao, Y.; Sun, Y.; Lv, X.; Liu, Y.L.; Chen, M.L.; Guo, W. Rhodamine-based chemosensor for Hg2+ in aqueous solution with a broad pH range and its application in live cell imaging. Org. Biomol. Chem. 2010, 8, 4143-4147.

25. Fang, Y.; Zhou, Y.; Rui, Q.; Yao, C. Rhodamine-ferrocene conjugate chemosensor for selectively sensing copper(II) with multisignals: Chromaticity, fluorescence, and electrochemistry and its application in living cell imaging. Organometallics 2015, 34, 2962-2970.

26. Zhou, J.; Yu, X.G.; Jin, X.L.; Tang, G.P.; Zhang, W.; Hu, J.M.; Zhong, C.F. Novel carbazole-based main chain polymeric metal complexes containing complexes of phenanthroline with Zn(II) or Cd(II): Synthesis, characterization and photovoltaic application in DSSCs. J. Mol. Struct. 2014, 1058, 14-21.

27. Cai, Z.B.; Liu, L.F.; Zhou, M. Synthesis of nickel(II) complexes containing modified phenanthroline ligands for potential nonlinear optical applications. Opt. Mater. 2013, 35, 1481-1486.

28. Bhattacharyya, B.; Kundu, A.; Guchhait, N.; Dhara, K. Anthraimidazoledione Based Reversible and Reusable Selective Chemosensors for Fluoride Ion: Naked-Eye, Colorimetric and Fluorescence "ON-OFF". J. Fluoresc. 2017, 27, 1041-1049.

29. Varadaraju, C.; Paulraj, M.S.; Tamilselvan, G.; Enoch, I.V.M.V.; Srinivasadesikan, V.; Shyi-Long, L. Evaluation of metal ion sensing behaviour of fluorescent probe along with its precursors: PET-CHEF mechanism, molecular logic gate behaviour and DFT studies. J. Incl. Phenom. Macro. 2019, 95, 79-89.

30. Zhang, S.; Yu, T.; Sun, M.; Yu, H.; Zhang, Z.; Wang, S.; Jiang, H. Highly sensitive and selective fluorescence detection of copper(II) ion based on multi-ligand metal chelation. Talanta 2014, 126, 185-190.

31. Jung, H.S.; Kwon, P.S.; Lee, J.W.; Kim, J.I.; Hong, C.S.; Kim, J.W.; Yan, S.; Lee, J.Y.; Lee, J.H.; Joo, T.; et al. Coumarin-derived Cu2+-selective fluorescence sensor: Synthesis, mechanisms, and applications in living cells. J. Am. Chem. Soc. 2009, 131, 2008-2012.

32. Wang, C.; Dong, B.; Kong, X.; Song, X.; Zhang, N.; Lin, W. A cancer cell-specific fluorescent probe for imaging Cu2+ in living cancer cells. Spectrochim. Acta A Mol. Biomol. Spectrosc. 2017, 182, 32-36.

33. Huang, W.; Zhou, P.; Yan, W.; He, C.; Xiong, L.; Li, F.; Duan, C. A bright water-compatible sugar-rhodamine fluorescence sensor for selective detection of Hg2+ in natural water and living cells. J. Environ. Monit. 2009, 11, 330-335.

34. Palanisamy, S.; Wang, Y.L.; Chen, Y.J.; Chen, C.Y.; Tsai, F.T.; Liaw, W.F.; Wang, Y.M. In vitro and in vivo imaging of nitroxyl with copper fluorescent probe in living cells and zebrafish. Molecules 2018, 23, 2551.

35. Yi, X.Q.; He, Y.F.; Cao, Y.S.; Shen, W.X.; Lv, Y.Y. Porphyrinic probe for fluorescence "turn-on" monitoring of Cu+ in aqueous buffer and mitochondria. ACS Sens. 2019, 4, 856-864.