Abstract/Details

Characterization of Ten Microbial Isolates from Two Serpentinite Seamounts, Asùt Tesoru and Fantangisña, in the Mariana Forearc

Shelton, Bronte.   University of California, San Diego ProQuest Dissertations Publishing,  2020. 28092212.

Abstract (summary)

The Mariana Forearc is home to the only known active serpentinite seamounts on Earth. Serpentinization, a reaction that occurs when ultramafic rock is exposed to water, fuels these unique environments. These seamounts are home to microbial communities that have barely begun to be explored; only one species of bacteria had been isolated and described prior to this research. During International Ocean Discovery Program Expedition 366, sediment samples were obtained from three seamounts. In this research, I characterized ten strains of bacteria isolated at atmospheric pressure from samples obtained from two of the seamounts, Asùt Tesoru and Fantangisña. All of the isolates are closely related to previously cultured microbes and represent three genera: Halomonas, Demequina, and Marinobacter. These ten isolates were examined for pH tolerance, pressure tolerance, salinity requirement and tolerance, and temperature tolerance. The majority of these isolates were pressure-sensitive and alkaliphilic. The only previously characterized bacterium isolated from the seamounts, Marinobacter alkalphilus str ODP1200D-1.5, was obtained from the Japan Collection of Microorganisms, tested alongside the isolates, and sent for genome sequencing along with three of the isolates. Genomic analyses revealed several adaptations and metabolic capabilities that could contribute to survival in the seamounts, including Na+/H+ antiporters and acetate metabolism. The results of this research indicate that the characterized isolates could be active in situ, and therefore likely represent a portion of the active community at the seamounts. This thesis contributes to the knowledge of the microbial communities and adaptations required for life at serpentinite seamounts.

Indexing (details)


Subject
Microbiology;
Aquatic sciences
Classification
0410: Microbiology
0792: Aquatic sciences
Identifier / keyword
Alkaliphiles; Extremophiles; Halomonas; Mariana Forearc; Marinobacter; Serpentinite
Title
Characterization of Ten Microbial Isolates from Two Serpentinite Seamounts, Asùt Tesoru and Fantangisña, in the Mariana Forearc
Author
Shelton, Bronte
Number of pages
84
Degree date
2020
School code
0033
Source
MAI 82/7(E), Masters Abstracts International
Place of publication
Ann Arbor
Country of publication
United States
ISBN
9798557084413
Advisor
Bartlett, Douglas H.
Committee member
Bowman, Jeff S.; Allen, Eric E.
University/institution
University of California, San Diego
Department
Scripps Institution of Oceanography
University location
United States -- California
Degree
M.S.
Source type
Dissertation or Thesis
Language
English
Document type
Dissertation/Thesis
Dissertation/thesis number
28092212
ProQuest document ID
2481109480
Copyright
Database copyright ProQuest LLC; ProQuest does not claim copyright in the individual underlying works.
Document URL
https://www.proquest.com/docview/2481109480