Differences the Effects of Tuak Dayak and Tuak Aren on the number of Leydig cells and seminiferous tubule thickness

Abstract

Background: Tuak Dayak and Tuak Aren are alcoholic drinks from the fermentation process. The material used by Tuak Dayak is black glutinous rice (Oryza sativa Linn) while Tuak Aren is made from Aren water (Arenga pinnata) tapping water. Infertility is related to ROS levels due to alcohol oxidative stress. The purpose of the study was to determine the difference in the effect of Tuak Dayak and Tuak Aren against the number of Leydig cells and the thickness of the seminiferous tubular tissue. Method: This study uses an experimental method to design a post-test only control group. Subjects were 25 rats divided into 5 groups with treatment for 4 weeks. Data are presented as standard deviation (mean ± SD). Furthermore the normality test (Shapiro Wilk) and homogeneity test (Levene Test). If the data is normally distributed and homogeneous, one way ANOVA test is performed. Post HOC / LSD test was performed to determine differences between groups (p-value ≤ 0.05). Then the Kruskal Wallis test if the distribution data is abnormal and not homogeneous and continued by the Mann Whitney test to know the difference between the two groups (p-value ≤ 0.05). Results: The number of Leydig cells in KTA₃ is significantly lower than KTD₁ (0.68) with a p-value of 0.020, this is also found in KTA₄ significantly lower than KTD₂ (0.920) with a p-value of 0.003, this study proves that Tuak Aren is more damaging than Tuak Dayak. While the thickness of the seminiferous tubules in KTA₃ is significantly lower than KTD₁ (0.49) with a p-value of 0.006, so also on KTA₄ is significantly lower than KTD₂ (0.71), it is evident that Tuak Aren also affects the depletion of seminiferous tubule thickness compared to Tuak Dayak. Conclusion: Significantly damage occurred in Leydig cells and depletion of seminiferous tubular tissue in test animals exposed to Tuak Aren more than Tuak Dayak. The more the number of Tuak drinks consumed the more cell damage that occurs.