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© 2021. This work is published under http://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.

Abstract

Objective

To investigate the role of physical activity in functional and molecular bladder alterations in an obese and insulin‐resistant murine model.

Methods

Wistar rats were randomized into 1. physical activity and standard diet; 2. physical activity and high‐fat diet; 3. no physical activity and standard diet; and 4. no physical activity and high‐fat diet. Groups 1 and 2 were subjected to a 10‐week swimming protocol. Urodynamic study (UDS) was performed, and the expression of genes in the bladder tissue related to the insulin pathway (IRS1/IRS2/PI3K/AKT/eNOS) was assessed using quantitative real‐time polymerase chain reaction.

Results

Groups 1 and 2 presented lower body weight gains than groups 3 (213.89 ± 13.77 vs 261.63 ± 34.20 grams (g), p = 0.04) and 4 (209.84 ± 27.40 vs 257.57 ± 32.95 g, p = 0.04), respectively. Group 4 had higher insulin level (6.05 ± 1.79 vs 4.14 ± 1.14 ng/ml, p = 0.038) and higher homeostasis model assessment of insulin resistance (HOMA‐IR) index (1.95 ± 0.73 vs 1.09 ± 0.37, p = 0.006) than group 1. On UDS, group 4 had greater number of micturition (13.6 ± 4.21 vs 6.0 ± 1.82, p = 0.04), higher postvoid pressure (8.06 ± 2.24 vs 5.08 ± 1.23, p = 0.04), lower capacity (0.29 ± 0.18 vs 0.91 ± 0.41 ml, p = 0.008), and lower bladder compliance (0.027 ± 0.014 vs 0.091 ± 0.034 ml/mmHg, p = 0.016) versus group 1. High‐fat diet was related to an underexpression throughout insulin signaling pathway, and physical activity was related to an overexpression of the pathway.

Conclusions

The insulin signaling pathway may be involved in the pathogenesis of bladder dysfunction related to a high‐fat diet. Physical activity may help to prevent bladder disfunction induced by a high‐fat diet through the insulin pathway.

Details

Title
Physical activity effects on bladder dysfunction in an obese and insulin‐resistant murine model
Author
Matos de Oliveira, André 1   VIAFID ORCID Logo  ; Fernando Mello Froes Fonseca 1 ; Reis, Sabrina Thalita 1 ; Viana, Nayara Izabel 1 ; Edilamar Menezes Oliveira 2 ; Luiz Osório Leiria 3 ; Katia Ramos Moreira Leite 1 ; Nahas, William Carlos 1 ; Srougi, Miguel 1 ; Antunes, Alberto Azoubel 1 

 Laboratory of Medical Research – LIM 55, Urology, University of Sao Paulo Medical School, Sao Paulo, Brazil 
 School of Physical Education and Sport, University of Sao Paulo, São Paulo, Brazil 
 Department of Pharmacology, Faculty of Medical Sciences, State University of Campinas, Campinas, Brazil 
Section
ORIGINAL ARTICLES
Publication year
2021
Publication date
Apr 2021
Publisher
John Wiley & Sons, Inc.
e-ISSN
2051817X
Source type
Scholarly Journal
Language of publication
English
ProQuest document ID
2516094347
Copyright
© 2021. This work is published under http://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.