A prognostic value of CD45RA+, CD45RO+, CCL20+ and CCR6+ expressing cells as ‘immunoscore’ to predict cervical cancer induced by HPV

Abstract

The interplay between cervical cancer (CC) and immune cells, mainly intratumoral lymphocytes, has a pivotal role in carcinogenesis. In this context, we evaluated the distribution of CD45RA⁺ and CD45RO⁺ cells as well as CCR6⁺ and CCL20⁺ cells in intraepithelial (IE) and marginal stroma (MS) areas from cervical intraepithelial neoplasia (CIN) I–III, and CC as ‘immunoscore’ for HPV-induced CC outcome. We observed increased CD45RA⁺ and CD45RO⁺ cells distribution in IE and MS areas in the CC group compared to CIN groups and healthy volunteers. Interestingly, there is a remarkable reduction of CCL20⁺ expressing cells distribution according to lesion severity. The CC group had a significant decrease in CCL20⁺ and CCR6⁺-expressing cells distribution in both IE and MS areas compared to all groups. Using the ‘immunoscore’ model, we observed an increased number of women presenting high CD45RA⁺/CD45RO⁺ and low CCL20⁺/CCR6⁺ ‘immunoscore’ in the CC group. Our results suggested a pattern in cervical inflammatory process with increasing CD45RA⁺/CD45RO⁺, and decreasing CCL20⁺/CCR6⁺ expression in accordance with CIN severity. Taken together, these markers could be evaluated as ‘immunoscore’ predictors to CC response. A more comprehensive analysis of longitudinal studies should be conducted to associate CD45RA⁺/CD45RO⁺ and CCL20⁺/CCR6⁺ ‘immunoscore’ to CC progression and validate its value as a prognosis method.