Abstract

Neurological manifestations are frequently reported in the COVID-19 patients. Neuromechanism of SARS-CoV-2 remains to be elucidated. In this study, we explored the mechanisms of SARS-CoV-2 neurotropism via our established non-human primate model of COVID-19. In rhesus monkey, SARS-CoV-2 invades the CNS primarily via the olfactory bulb. Thereafter, viruses rapidly spread to functional areas of the central nervous system, such as hippocampus, thalamus, and medulla oblongata. The infection of SARS-CoV-2 induces the inflammation possibly by targeting neurons, microglia, and astrocytes in the CNS. Consistently, SARS-CoV-2 infects neuro-derived SK-N-SH, glial-derived U251, and brain microvascular endothelial cells in vitro. To our knowledge, this is the first experimental evidence of SARS-CoV-2 neuroinvasion in the NHP model, which provides important insights into the CNS-related pathogenesis of SARS-CoV-2.

Details

Title
The olfactory route is a potential way for SARS-CoV-2 to invade the central nervous system of rhesus monkeys
Author
Jiao, Li 1 ; Yang, Yun 1 ; Yu Wenhai 1 ; Zhao, Yuan 1 ; Long Haiting 1 ; Gao Jiahong 1 ; Ding Kaiyun 1 ; Ma Chunxia 1 ; Li, Jingmei 1 ; Zhao Siwen 1 ; Wang Haixuan 1 ; Li, Haiyan 1 ; Yang Mengli 1 ; Xu Jingwen 1 ; Wang, Junbin 1 ; Yang, Jing 1 ; Kuang Dexuan 1 ; Luo Fangyu 1 ; Qian Xingli 1 ; Xu Longjiang 1 ; Yin Bin 2 ; Liu, Wei 3 ; Liu, Hongqi 1 ; Lu Shuaiyao 4 ; Peng Xiaozhong 4   VIAFID ORCID Logo 

1  Chinese Academy of Medical Sciences and Peking Union Medical College, National Kunming High-Level Biosafety Primate Research Center, Institute of Medical Biology, Kunming, China (GRID:grid.506261.6) (ISNI:0000 0001 0706 7839) 
2  Chinese Academy of Medical Sciences, School of Basic Medicine Peking Union Medical College, State Key Laboratory of Medical Molecular Biology, Department of Molecular Biology and Biochemistry, Institute of Basic Medical Sciences, Medical Primate Research Center, Neuroscience Center, Beijing, China (GRID:grid.506261.6) (ISNI:0000 0001 0706 7839) 
3  Chinese Academy of Medical Sciences, School of Basic Medicine Peking Union Medical College, Department of Anatomy, Institute of Basic Medical Sciences, Beijing, China (GRID:grid.506261.6) (ISNI:0000 0001 0706 7839) 
4  Chinese Academy of Medical Sciences and Peking Union Medical College, National Kunming High-Level Biosafety Primate Research Center, Institute of Medical Biology, Kunming, China (GRID:grid.506261.6) (ISNI:0000 0001 0706 7839); Chinese Academy of Medical Sciences, School of Basic Medicine Peking Union Medical College, State Key Laboratory of Medical Molecular Biology, Department of Molecular Biology and Biochemistry, Institute of Basic Medical Sciences, Medical Primate Research Center, Neuroscience Center, Beijing, China (GRID:grid.506261.6) (ISNI:0000 0001 0706 7839) 
Publication year
2021
Publication date
2021
Publisher
Nature Publishing Group
ISSN
20959907
e-ISSN
20593635
Source type
Scholarly Journal
Language of publication
English
DOI
https://doi.org/10.1038/s41392-021-00591-7
ProQuest document ID
2517673658
Copyright
© The Author(s) 2021. This work is published under http://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.