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© 2022 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.

Abstract

Lesion mimic mutants provide ideal genetic materials for elucidating the molecular mechanism of cell death and disease resistance. The maize necrotic leaf mutant (nec-t) is a recessive mutant with necrotic spots and yellow-green leaves. In this study, we found that nec-t was a light and temperature-dependent mutant. Map-based cloning and the allelic test revealed that nec-t was a novel allelic mutant of the Necrotic4 gene. Necrotic4 encodes the coproporphyrinogen III oxidase (CPX1), a key enzyme in the tetrapyrrole pathway, catalyzing coproporphyrinogen III oxidate to protoporphyrinogen IX. Subcellular localization showed that the necrotic4 protein was localized in the chloroplast. Furthermore, RNA-seq analysis showed that the Necrotic4 mutation caused the enhanced chlorophyll degradation and reactive oxygen species (ROS) response. The mechanism of plant lesion formation induced by light and temperature is not clear. Our research provides a basis for understanding the molecular mechanism of necrosis initiation in maize.

Details

Title
A Maize Necrotic Leaf Mutant Caused by Defect of Coproporphyrinogen III Oxidase in the Porphyrin Pathway
Author
Zhao, Yan 1 ; Xu, Wei 2 ; Wang, Lijing 3 ; Han, Shuai 2 ; Zhang, Yongzhong 2 ; Liu, Qingzhi 2 ; Liu, Baoshen 2 ; Zhao, Xiangyu 4 

 College of Life Sciences/State Key Laboratory of Crop Biology, Shandong Agricultural University, Tai’an 271018, China; [email protected]; College of Agronomy/State Key Laboratory of Crop Biology, Shandong Agricultural University, Tai’an 271018, China; [email protected] (W.X.); [email protected] (S.H.); [email protected] (Y.Z.); [email protected] (Q.L.) 
 College of Agronomy/State Key Laboratory of Crop Biology, Shandong Agricultural University, Tai’an 271018, China; [email protected] (W.X.); [email protected] (S.H.); [email protected] (Y.Z.); [email protected] (Q.L.) 
 College of Life Sciences, De Zhou University, Dezhou 253023, China; [email protected] 
 College of Life Sciences/State Key Laboratory of Crop Biology, Shandong Agricultural University, Tai’an 271018, China; [email protected] 
First page
272
Publication year
2022
Publication date
2022
Publisher
MDPI AG
e-ISSN
20734425
Source type
Scholarly Journal
Language of publication
English
ProQuest document ID
2632737381
Copyright
© 2022 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.