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Abstract
SARS-CoV-2 is the virus that causes the disease called COVID-19, which has caused the worst pandemic of the century. Both, to know the immunological status of general population and to evaluate the efficacy of the vaccination process that is taking place around the world, serological tests represent a key tool. Classic serological tests, based on colorimetric techniques, such as ELISA or CLIA, continue to be the most widely used option. However, a real improvement in results is still needed. We developed a highly sensitive and specific FCM assay that allows the detection of IgG and IgA antibodies, directed against the native and functional S-protein of SARS-CoV-2 exposed on the membrane of a transfected cell line, up to 8 months after infection.
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Details
1 ISABIAL, Hospital General de Alicante, Alicante, Spain (GRID:grid.411086.a) (ISNI:0000 0000 8875 8879)
2 Hospital General de Alicante, Alicante, Spain (GRID:grid.411086.a) (ISNI:0000 0000 8875 8879)
3 Centro de Biología Molecular Severo Ochoa, Madrid, Spain (GRID:grid.465524.4)
4 Hospital General de Valencia, Valencia, Spain (GRID:grid.106023.6) (ISNI:0000 0004 1770 977X)