Content area
Full text
ABSTRACT:
The main objective of this study was to evaluate in vitro antilithiatic activity of ethanolic extract of roots of Ipomoea digitata Linn. Inhibitory effect of extract on in vitro crystallization was analysed by nucleation assay. Cystone tablet a marketed drug used as a standard drug which is dispersed into 100ml buffer solution for compare of various concentration of ethanolic root extract of Ipomoea digitata Linn. The inhibitory activity of root extract on the nucleation of calcium oxalate crystals and the rate of aggregation in calcium oxalate crystals was determined by spectrophotometric assay. Investigation revealed that ethanolic extract of roots of Ipomoea digitata Linn is positive effect on inhibition for formatting precipitation of calcium and potassium in vitro.
KEYWORDS: Antilithiatic, Ipomoea digitata Linn, urolithiasis, Calcium oxalate, aggregation.
INTRODUCTION:
Stone formation is the oldest and serious painful urologic disease with significant prevalence in the population due to change in lifestyle and dietary factors. The risk of developing urolithiasis have been reported in adults appears as in western countries which are 5 - 9% in Europe, 12% in Canada, and 13-15% in the USA and also in some Asian countries such as Saudi Arabia 20.1% with lifetime recurrence rates of up to 50%.1-4 According to an estimate every year 600000 Americans suffer from urinary stone. In India about 12% of Indian suffers out of which 50% from urinary stone.5 Urolithiasis is a complex process which is created from imbalance between inhibitors and promoters in the kidney. Stone formation or lithiasis is characterized by calculi formation. It has two types such as nephrolithiasis and urolithiasis. Calculi formation in urinary bladder, ureter or any part of urinary tract rather than kidney is known as urolithiasis while nephrolithiasis is characterized calculi formation in kidney. It is the solid non -metallic minerals found in the urinary track.
It is a stone which is formed by an aggregation of solute materials from urine such as calcium, oxalate, phosphate and uric acid. The formation of lithiasis involves several physiochemical events which is beginning with super saturation, crystal nucleation, crystal growth, aggregation and end with retention within the urinary tract. The most common calcium oxalate stones represent up to 80% of the analysed stones.
Ipomoea digitata (Convolvulaceae) is perennial medicinal herb which is...