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Copyright © 2021 Di Deng et al. This work is licensed under http://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.

Abstract

As part of the inner ear, the vestibular system is responsible for sense of balance, which consists of three semicircular canals, the utricle, and the saccule. Increasing evidence has indicated that the noncanonical Wnt/PCP signaling pathway plays a significant role in the development of the polarity of the inner ear. However, the role of canonical Wnt signaling in the polarity of the vestibule is still not completely clear. In this study, we found that canonical Wnt pathway-related genes are expressed in the early stage of development of the utricle and change dynamically. We conditionally knocked out β-catenin, a canonical Wnt signaling core protein, and found that the cilia orientation of hair cells was disordered with reduced number of hair cells in the utricle. Moreover, regulating the canonical Wnt pathway (Licl and IWP2) in vitro also affected hair cell polarity and indicated that Axin2 may be important in this process. In conclusion, our results not only confirm that the regulation of canonical Wnt signaling affects the number of hair cells in the utricle but also provide evidence for its role in polarity development.

Details

Title
Canonical Wnt Signaling Pathway on Polarity Formation of Utricle Hair Cells
Author
Deng, Di 1   VIAFID ORCID Logo  ; Qian, Xiaoqing 2   VIAFID ORCID Logo  ; Chen, Binjun 2   VIAFID ORCID Logo  ; Yang, Xiaoyu 2   VIAFID ORCID Logo  ; Wang, Yanmei 2   VIAFID ORCID Logo  ; Fanglu Chi 2   VIAFID ORCID Logo  ; Huang, Yibo 2   VIAFID ORCID Logo  ; Zhao, Yu 3   VIAFID ORCID Logo  ; Ren, Dongdong 2   VIAFID ORCID Logo 

 Department of Otolaryngology, Head and Neck Surgery, West China Hospital, Sichuan University, Chengdu 610041, China; NHC Key Laboratory of Hearing Medicine (Fudan University), Shanghai 200031, China 
 NHC Key Laboratory of Hearing Medicine (Fudan University), Shanghai 200031, China; ENT Institute and Department of Otorhinolaryngology, Eye & ENT Hospital, Fudan University, Shanghai 200031, China 
 Department of Otolaryngology, Head and Neck Surgery, West China Hospital, Sichuan University, Chengdu 610041, China 
Editor
Renjie Chai
Publication year
2021
Publication date
2021
Publisher
John Wiley & Sons, Inc.
ISSN
20905904
e-ISSN
16875443
Source type
Scholarly Journal
Language of publication
English
ProQuest document ID
2537374538
Copyright
Copyright © 2021 Di Deng et al. This work is licensed under http://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.