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© 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.

Abstract

[...]if single cells can survive in the developing embryo without senescence and without reprogramming, stalled in their state and not eliminated from rapidly growing foetuses, their number would be extremely low (below the detection threshold), as only three to four cells are introduced into the embryo. [...]the presence of donor DNA in the brain and heart cells of adult mice are evidence of the ectodermal and mesodermal lineages, respectively, of those cells. [...]studying the re-establishing of physical connections inside morula after MEFs are placed in between blastomeres would be promising in the search for the reprogramming trigger. “…cell microinjection destroys one or more blastomeres in the host embryos, resulting in the release of cytoplasmic determinants in the embryonic environment and triggering reprogramming in a process comparable with SCNT.” [...]triggering reprogramming in the process comparable with SCNT” is simply not possible, as the contact of injected cell nucleus and recipient cytoplasm is required for reprogramming by somatic cell nuclear transfer. Again, the integral fibroblast cell membrane prevents the penetration of released blastomere’s content. [...]we can exclude also that possibility from potential mechanisms of somatic cell reprogramming in morula.

Details

Title
Introduction of Mouse Embryonic Fibroblasts into Early Embryos: From Confusion to Constructive Discussion. Comment on Savatier, P. Introduction of Mouse Embryonic Fibroblasts into Early Embryos Causes Reprogramming and (Con)fusion. Cells 2021, 10, 772
Author
Żyżyńska-Galeńska, Krystyna 1 ; Karasiewicz, Jolanta 1 ; Bernat, Agnieszka 2   VIAFID ORCID Logo 

 Department of Experimental Embryology, Institute of Genetics and Animal Biotechnology, Polish Academy of Sciences, Jastrzębiec, 05-552 Magdalenka, Poland; [email protected] (K.Ż.-G.); [email protected] (J.K.) 
 Department of Experimental Embryology, Institute of Genetics and Animal Biotechnology, Polish Academy of Sciences, Jastrzębiec, 05-552 Magdalenka, Poland; [email protected] (K.Ż.-G.); [email protected] (J.K.); Laboratory of Molecular Diagnostics, Department of Biotechnology, Intercollegiate Faculty of Biotechnology, University of Gdańsk & Medical University of Gdańsk, 80-307 Gdańsk, Poland 
First page
1534
Publication year
2021
Publication date
2021
Publisher
MDPI AG
e-ISSN
20734409
Source type
Scholarly Journal
Language of publication
English
ProQuest document ID
2544645062
Copyright
© 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.