Abstract

Background

Verticillium wilt is a serious soil-borne vascular disease that causes major losses to upland cotton (Gossypium hirutum L.) worldwidely every year. The protein VIP1 (VirE2 interaction protein 1), a bZIP transcription factor, is involved in plant response to many stress conditions, especially pathogenic bacteria. However, its roles in cotton response to Verticillium wilt are poorly understood.

Results

The GbVIP1 gene was cloned from resistant sea-island cotton (G. barbadense) cv. Hai 7124. Expression of GbVIP1 was up-regulated by inoculation with Verticillium dahliae and exogenous treatment with ethylene. Results of virus-induced gene silencing suggested that silencing of GbVIP1 weakened cotton resistance to Verticillium wilt. The heterologous expression of GbVIP1 in tobacco showed enhanced resistance to Verticillium wilt. The PR1, PR1-like and HSP70 genes were up-regulated in GbVIP1 transgenic tobacco after Verticillium wilt infection.

Conclusion

Our results suggested that GbVIP1 increased plant resistance to Verticillium wilt through up-regulating expressions of PR1, PR1-like, and HSP70. These results provide new approaches to improving resistance to Verticillium wilt in upland cotton and also have great potential for disease-resistance breeding of cotton.

Details

Title
Isolation and characterization of the GbVIP1 gene and response to Verticillium wilt in cotton and tobacco
Author
ZHANG, Kai; ZHAO, Pei; WANG, Hongmei; ZHAO, Yunlei; CHEN, Wei; GONG, Haiyan; SANG, Xiaohui; CUI, Yanli
Pages
1-11
Section
Research
Publication year
2019
Publication date
2019
Publisher
Springer Nature B.V.
ISSN
20965044
e-ISSN
25233254
Source type
Scholarly Journal
Language of publication
English
DOI
https://doi.org/10.1186/s42397-019-0019-0
ProQuest document ID
2546829830
Copyright
© 2019. This work is licensed under http://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.