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© 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.

Abstract

Gingival recession (GR) potentially leads to the exposure of tooth root to the oral cavity microenvironment and increases susceptibility to dental caries, dentin hypersensitivity, and other dental diseases. Even though many etiological factors were reported, the specific mechanism of GR is yet to be elucidated. Given the species richness concerning marine biodiversity, it could be a treasure trove for drug discovery. In this study, we demonstrate the effects of a marine compound, (+)-rhodoptilometrin from crinoid, on gingival cell migration, wound healing, and oxidative phosphorylation (OXPHOS). Experimental results showed that (+)-rhodoptilometrin can significantly increase wound healing, migration, and proliferation of human gingival fibroblast cells, and it does not have effects on oral mucosa fibroblast cells. In addition, (+)-rhodoptilometrin increases the gene and protein expression levels of focal adhesion kinase (FAK), fibronectin, and type I collagen, changes the intracellular distribution of FAK and F-actin, and increases OXPHOS and the expression levels of complexes I~V in the mitochondria. Based on our results, we believe that (+)-rhodoptilometrin might increase FAK expression and promote mitochondrial function to affect cell migration and promote gingival regeneration. Therefore, (+)-rhodoptilometrin may be a promising therapeutic agent for GR.

Details

Title
Rhodoptilometrin, a Crinoid-Derived Anthraquinone, Induces Cell Regeneration by Promoting Wound Healing and Oxidative Phosphorylation in Human Gingival Fibroblast Cells
Author
Tseng, Chung-Chih 1   VIAFID ORCID Logo  ; Yu-Cheng, Lai 2 ; Tsu-Jen Kuo 3 ; Jui-Hsin Su 4 ; Ping-Jyun Sung 4   VIAFID ORCID Logo  ; Chien-Wei, Feng 5 ; Yen-You, Lin 6 ; Pei-Chin, Chen 7 ; Ming-Hong, Tai 8 ; Shu-Yu, Cheng 7 ; Hsiao-Mei Kuo 9   VIAFID ORCID Logo  ; Zhi-Hong, Wen 7 

 Department of Marine Biotechnology and Resources, National Sun Yat-sen University, Kaohsiung 80424, Taiwan; Department of Dentistry, Zuoying Branch of Kaohsiung Armed Forces General Hospital, Kaohsiung 81357, Taiwan 
 Department of Marine Biotechnology and Resources, National Sun Yat-sen University, Kaohsiung 80424, Taiwan; Department of Orthopedics, Kaohsiung Veterans General Hospital, Kaohsiung 81362, Taiwan 
 Department of Marine Biotechnology and Resources, National Sun Yat-sen University, Kaohsiung 80424, Taiwan; Department of Stomatology, Kaohsiung Veterans General Hospital, Kaohsiung 81362, Taiwan 
 Graduate Institute of Marine Biology, National Dong Hwa University, Pingtung 944, Taiwan; National Museum of Marine Biology and Aquarium, Pingtung County 94450, Taiwan 
 Department of Marine Biotechnology and Resources, National Sun Yat-sen University, Kaohsiung 80424, Taiwan 
 Department of Orthopedic Surgery, Ping-Tung Christian Hospital, Pingtung 90059, Taiwan 
 Department of Marine Biotechnology and Resources, National Sun Yat-sen University, Kaohsiung 80424, Taiwan; Doctoral Degree Program in Marine Biotechnology, National Sun Yat-sen University, Kaohsiung 80424, Taiwan 
 Center for Neuroscience, National Sun Yat-sen University, Kaohsiung 80424, Taiwan; Institute of Biomedical Sciences, National Sun Yat-sen University, Kaohsiung 80424, Taiwan 
 Department of Marine Biotechnology and Resources, National Sun Yat-sen University, Kaohsiung 80424, Taiwan; Center for Neuroscience, National Sun Yat-sen University, Kaohsiung 80424, Taiwan 
First page
138
Publication year
2019
Publication date
2019
Publisher
MDPI AG
e-ISSN
16603397
Source type
Scholarly Journal
Language of publication
English
ProQuest document ID
2548650852
Copyright
© 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.