DNA Binding three Azo Dyes as new Antibiotics

Abstract

The antimicrobial activities of (E)-4-((2-nitrophenyl) diazenyl) benzene-1,3-diol (1), (E)-4-((3-nitrophenyl) diazenyl) benzene-1,3-diol (2) and (E)-4-((4-nitrophenyl) diazenyl) benzene-1,3-diol (3), were screened in vitro towered Staphylococcus aureus strain NCTC 6571 and Candida Krusei. The results were revealed that the azo dye (2), which has NO₂ group in the meta-position, was more active towered Staphylococcus aureus using 0.3 mg / mL and 0.4 mg/ mL concentrations. This activity was seemed to be higher than the activity of (1) and (3), which have substituted NO₂ group in ortho-and para-positions respectively. However, the azo dye (1) was gave well activity against Candida Krusei than (2) and (3) using same concentrations. These results were then compared with that obtained by antibiotics (erythromycin capsules, amoxicillin capsules and metheprim tablet). The results were displayed that the activities of different concentrations of each azo dye were better than the antibiotics in the treatment of the disseminated infection. Further, the three azo dyes were showed non-toxic effects toward of the hemolytic red blood cells and didn’t show any hemolysis effect in the cells. Further the three azo dyes were variable in their bonded to the infected human DNA. The azo dyes (1) and (3) can affect the human DNA resulting DNA damage, which inhibits DNA transcription and replication. However, the azo dye (2) was right bind the human DNA without damaged it in contrast with the control. Due to recommend the three synthetic azo dyes as new antibiotics for disseminated infection. The first section in your paper