The Antibacterial activity of Spirulina platensis aqueous extract and Chitosan nanoparticles on bacterial isolates from different human Sources

Abstract

This study has involved the test of antibacterial activity for Spirulina platensis aqueous extract (SPAE) and Chitosan nanoparticles (CHNPs) towards Pseudomonas aeruginosa, Klebsiella pneumoniae, Escherichia coli, Sphingomonas paucimobilis, Staphylococcus aureus, Streptococcus pneumoniae and Granulicatella adicans that isolated from different infection sources. The results were showed that 200mg/ml of SPAE showed an ability to inhibition of bacterial isolates at inhibition zone diameters IZD at 22, 20, 25, 13, 11, 26 and 11 mm. While the 100mg/ml of CHNPs was inhibited effects at 37, 29, 27, 37, 38, 41 and 36 mm respectively. The Minimum Inhibitory Concentration MIC of SPAE against P. aeruginosa, K.pneumonia, E. coli, S. pneumonia was at 50%, while S. paucimobilis, G. adicans and S. aureus were at 100%. The used of 75mg/ml of SPAE or 12.5 mg/ml from CHNPs have significant synergism inhibition effects with each 25 μg Amoxicillin, 10 μg Ciprofloxacin and 15 μg of Azithromycin against the bacterial isolates compared with the antibiotic actions alone.

The conclusion was showed that SPAE and CHNPs has inhibiton activity against the bacterial isolates and increased significantly when mixture with the antibiotics.