Abstract

The generation of micro-droplets of nutrient medium with living cells by pulsed electrohydrodynamic printing has been studied. In-situ visualization by high-speed camera made it possible to measure the characteristic times of droplet generation process and to determine the optimal printing parameters. Maximal frequency of stable generation was achieved at 700 Hz. This technique was applied successfully for drop-on-demand printing of culture medium with live HeLa cells and yeasts.

Details

Title
High-speed precise cell patterning by pulsed electrohydrodynamic jet printing
Author
Makaev, A V 1 ; Mingaliev, E A 1 ; Karpov, V R 1 ; Zubarev, I V 1 ; V Ya Shur 1 ; O S El’kina 1 

 Ural Federal University, Ekaterinburg, 620002, Russia 
Publication year
2017
Publication date
Oct 2017
Publisher
IOP Publishing
ISSN
17578981
e-ISSN
1757899X
Source type
Scholarly Journal
Language of publication
English
ProQuest document ID
2564400440
Copyright
© 2017. This work is published under http://creativecommons.org/licenses/by/3.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.