Structures and Biological Activities of Diketopiperazines from Marine Organisms: A Review

Abstract

Diketopiperazines are potential structures with extensive biological functions, which have attracted much attention of natural product researchers for a long time. These compounds possess a stable six-membered ring, which is an important pharmacophore. The marine organisms have especially been proven to be a wide source for discovering diketopiperazine derivatives. In recent years, more and more interesting bioactive diketopiperazines had been found from various marine habitats. This review article is focused on the new 2,5-diketopiperazines derived from marine organisms (sponges and microorganisms) reported from the secondary half-year of 2014 to the first half of the year of 2021. We will comment their chemical structures, biological activities and sources. The objective is to assess the merit of these compounds for further study in the field of drug discovery.

1. Introduction

The 2,5-diketopiperazines (DKPs), the smallest cyclic dipeptides from the double condensations of two α-amino acids, are abundant in nature and possess a six-membered piperazine rigid backbone [1,2]. The formation of two peptide bonds in DKPs are catalyzed by two major enzymes, the nonribosomal peptide synthetases (NRPSs, >100 kDa) and cyclodipeptide synthases (CDPSs, 30 kDa) [3]. These three-dimensional molecular skeletons carry different substituents, which overcome the planar limitations of most conventional drugs and play an important role in drug discovery [4]. Recently, the interest in DKPs is still high because they have not only antimicrobial, antitumor and antiviral activities [5], but also a relatively rare treatment of ischemic brain injury [6], quorum-sensing signaling [7,8], anti-Alzheimer [9], inhibition of microtubule polymerization [10] and haemosuppressor activity [11].

Bicyclomycin is a classic DKP antibiotic that has been used to treat diarrhea in humans and bacterial diarrhea in calves and pigs and it is also a rho (a member of the RecA-type ATPase) inhibitor [12,13,14]. Chaetocin is a specific DKP dimer containing sulfurs as the first inhibitor of a lysine-specific histone methyltransferase SU(VAR)3-9, which could reduce the H3 isoform trimethylated at the Lys9 (H3K9me3) level and this compound has also been reported to have potent antimyeloma activity [15,16,17]. Recently, chaetocin is proved to be able to increase the efficiency of the efficient epigenetic reprogramming via reducing the aberrant level of H3K9me3 to enhance the developmental competence of porcine somatic cell nuclear transfer embryos. It promotes osteogenic differentiation in mesenchymal stem cells [16,18,19]. Plinabulin (formerly named as NPI-2358), a marine-derived DKP, is currently in a phase 3 trial in combination with docetaxel in non-small cell lung cancer (NCT02504489) [20,21,22]. In addition, some DKPs possess an ability to cross the blood–brain barrier via a passive diffusion process as an ideal candidate for new therapeutic agents for brain diseases [23,24]. As of August 2012, there are approximately 150 global patents related to DKPs and its derivatives, and DKPs are present in nearly 50 bio-complexes in the Research Collaboratory for the Structural Bioinformatics Protein Data Bank [25,26]. DKPs are diamonds in the rough and have huge potential in future therapies.

There have been several reviews reported on DKPs until now. Cao et al. summarized chemical diversity and the biological function of indolediketopiperazines from marine-derived fungi [27]. Gomes et al. summed up marine-derived DKP dimers with their structures and biological activity [28]. From 1972 to the first half of the year of 2014, 214 DKPs from marine sources have been reported [5,29]. However, there is no relevant review for summarizing the comprehensive DKPs from a marine source from 2014 to now. On this basis, we now summarize a total of 241 marine-derived DKPs from the second half of 2014 to the first half of 2021 in this paper. In addition, a total of 55 marine-derived variable DKP derivatives from 2011 to the first half of 2021 are also summarized here.

2. Chemical Structures of Diketopiperazines from Marine Organisms

The 241 DKPs from different sources including sponges, bacteria, actinomycetes and fungi are shown in Table 1.

2.1. Sponge

Cyclo-(R-Pro-6-hydroxyl-S-Ile) (1) was a new DKP isolated from the sponge Callyspongia sp., which was collected from the South China Sea. Compound 1 showed no antibacterial activity against the tested Bacillus subtilis, Staphylococcus aureus and Escherichia coli [30]. Geobarrettins A (2) and B (3), two new DKPs, were obtained from the sub-Arctic sponge Geodia barrette, which was collected at the west of Iceland (-388 m). Before coculturing with allogeneic CD4⁺ T cells, the maturing human dendritic cells (DCs) were processed by compound 3 with a dose of 10 μg/mL and then reduced the IFN-γ of T cell secretion. Compound 3 had no effect on the DCs secretion of IL-10 but induced the IL-12p40. This above data demonstrated that compound 3 possessed an overall anti-inflammatory activity and may be used to treat the Th1 type inflammation [31]. A novel sulfur-containing DKP, tedanizaine A (4), was collected from the marine sponge Tedania sp. at a depth of 10 m in Zhanjiang, Guangdong province. Compound 4 bearing a thiazolidine unit was separated by integrating molecular networking and became the second example of thiodiketopiperazine. However, the evaluation of cytotoxicity activities did not reveal the inhibitory activity of compound 4 on the growth of the tested A549 (lung carcinoma) and RAW 246.7 (macrophage) cell lines [32]. (−)-Cyclo(L-trans-Hyp-L-Ile) (5) was a new DKP isolated from the marine sponge Axinella sinoxea, collected from a reef habitat around Larak Island, Persian Gulf. Compound 5 had no influence on methicillin-resistant Staphylococcus aureus (MRSA) in the dose of 100 μg/mL [33]. All 5 DKPs from sponge described above are presented in Figure 1.

2.2. Bacteria

Cyclo (Trp-Ser) (6) was a novel DKP from Rheinheimera aquimaris QSI02, in the Yellow Sea of Qingdao, which was active against E. coli, Chromobacterium violaceum CV026 and Pseudomonas aeruginosa PA01 with the minimum inhibitory concentration (MIC) values of 6.4, 3.2 and 6.4 mg/mL, respectively. Compound 6 possessed antiquorum sensing activity, which could decrease the QS-regulated violacein and pyocyanin production by 67% and 65% in C. violaceum CV026 and P. aeruginosa. Based on the molecular docking results, compared with the natural signaling molecule, compound 6 was easier to combine with the CviR receptor, but opposite in the LasR receptor. These consequences indicated that compound 6 may become a potential inhibitor to control the quorum sensing (QS) system [34]. Four novel DKPs were isolated from psychrophilic yeast Glaciozyma antarctica PI12, which was collected from a marine environment in Antarctica, and they were named as cyclo(Pro-Val) (7), (−)-cyclo(Pro-Tyr) (8), (−)-cyclo(Pro-Phe) (9) and (+)-cyclo(Pro-Leu) (10). However, all these compounds reported in the present study were not subjected to further bioactivity studies [35]. (3S,6S)-3,6-diisobutylpiperazine-2,5-dione (11) was firstly displayed from a sponge-associated bacterium Bacillus sp. SPB7, which was isolated from the sponge Spongia officinalis. Compound 11 exhibited antimicrobial activity against E. coli and S. aureus subsp. aureus with the MIC values of 16 and 22 μg/mL, respectively [36]. Gallaecimonamides A–C (12–14) were three new DKPs collected from the marine bacterium Gallaecimonas mangrovi HK-28, which was isolated from the mangrove sediment from Haikou, Hainan Province, China. Compound 12 showed significant selectively antimicrobial activity against V. harveyi with the MIC value of 50 μM [37]. Three novel chlorine-containing DKPs namely cis-cyclo(Pro-3-chloro-Tyr) (15), trans-cyclo(Pro-3-chloro-Tyr) (16) and cis-cyclo(3-chloro-Tyr-Ile) (17) were isolated from Bacillus subtilis BI0980 collected from a depth of 18 m marine sediment between the islands of Kerkyra and Erikoussa. Compounds 15 and 16 showed no inhibitory activity for the tested fungi (Candida albicans and Aspergillus niger) [38]. All 12 DKPs from bacteria described above are presented in Figure 1.

2.3. Actinomycetes

A novel DKP, iso-naseseazine B (18), was isolated from the medium of Streptomyces sp. SMA-1 from the marine sediment of the Yellow Sea, China. Compound 18 could oppose the fluconazole-resistant C. albicans and the diameter of the inhibition zone was 9 mm [39]. The other Streptomyces sp. USC-636 strain was collected from marine sediment in Sunshine Coast, QLD, Australia. Naseseazine C (21) was extracted from the culture of this strain, which exhibited a novel C-6’/C-3 linkage between the two DKP subunits and possessed the activity of antiplasmodial with an IC₅₀ value of 3.52 μM. However, the analog naseseazines A (19) and B (20) inhibited the malaria parasite at a dose of 20 μM. The special linkage between C-6′ and C-3 may be critical to increase bioactivity [40]. (6R,3Z)-3-benzylidene-6-isobutyl-1-methyl piperazine-2,5-dione (22), a new DKP, was produced by the Streptomyces sp. strain SCSIO 04496, which was collected from a deep-sea sediment sample of the South China Sea [41]. ΔmarF, the methyltransferase gene of maremycins, knockout in strain Streptomyces sp. B9173 (collected from the pacific coast of Chile) obtained the mutant LS26. Six new demethylmaremycins (23–28) were isolated from the mutant LS26, however, the specific name of these compounds could not be found [42]. Streptomyces sp. MNU FJ-36 was collected from the Katsuwonus sp. intestinal fabric. Three novel compounds, 3-(3-hydroxy-4-methoxybenzyl)-6-isobutyl-2,5-diketopiperazine (29), 3-(1,3-benzodioxol-5-ylmethyl)-6-isobutyl-2,5-diketopiperazine (30) and 3-(1,3-benzodioxol-5-ylmethyl)-6-isopropyl-2,5-diketopiperazine (31), were isolated from the Streptomyces sp. MNU FJ-36. All of these compounds 29–31 could inhibit the growth of the A549 cell lines with IC₅₀ values of 89.4, 35.4 and 28.4 mg/mL, respectively. Compounds 30 and 31 also exhibited a weak cytotoxicity against the HCT-116 (human colon carcinoma) cell lines with IC₅₀ values of 75.4 and 45.4 mg/mL, respectively [43]. A novel DKP glycosidem, maculosin-O-α-L-rhamnopyranoside (32), was discovered from the Streptomyces sp. ZZ446, which was collected in coastal soil from Zhoushan Islands, Zhejiang Province. Compound 32 possessed antimicrobial activity against MRSA, E. coli and C. albicans with MIC values of 37, 28 and 26 μg/mL, respectively [44,45]. Actinozine A (33) was a new DKP, which was isolated from the Streptomyces species Call-36 from the Red Sea sponge Callyspongia species. Compound 33 exhibited a moderate antimicrobial activity against S. aureus and C. albicans with inhibition zones of 23 and 19 mm at 100 μg/disc, respectively, and showed a weak activity for the HCT-116 (IC₅₀ = 146 μM) and MCF-7 (breast cancer, IC₅₀ = 88.8 μM) cell lines [46]. Streptomyces sp. SY1965 was collected from the Mariana Trench sediment-associated at a depth of 11,000 m and two new DKPs, streptodiketopiperazines A (34) and B (35) were isolated from the strain. The crude extraction of this strain from the Gauze’s liquid medium with sea salt could suppress the human glioma U87MG and U251 cells with an inhibition rate of over 100%. Both compounds 34 and 35 exhibited antifungal activity against C. albicans with a MIC value of 42 μg/mL [47].

A novel DKP, cyclo-(4-trans-6-dihydroxy-proline-D-leucine) (36), was discovered from the Microbulbifer variabilis C-03, which isolated from the Palythoa tuberculosa in the intertidal zone of Wanlitong [48]. One novel DKP namely nocarazepine A (37) was isolated from the Nocardiopsis alba collected from the gorgonian Anthogorgia caerulea, which was sampled from the coast of Xieyang Island, Guangxi Province [49]. Strain AJS-327 was a rare actinomycete (maybe a new linkage within Streptomycetaceae) and collected from the sponge fragment on the beach from La Jolla, CA. It was proved to be a likely novel species because it exhibited an extremely poor 16S rRNA sequence similarity to other members of the actinomycete family Streptomycetaceae. Four new DKPs, photopiperazines A–D (38–41), were isolated from this strain. The olefin geometrical isomers of these four compounds could be interconverted under light conditions, on account of the photopiperazines being sensitive to light. In terms of activity, the mixture of four compounds included compounds 38 (33.5%), 39 (39.7%), 40 (8.4%) and 41 (18.4%). This mixture exhibited a remarkable activity to the cancer cell of U87 (glioblastoma brain cancer), SHOV3 (ovarian cancer), MDA-MB-231(breast cancer) and HCT116 (human colon carcinoma) with IC₅₀ values of 1.2 × 10⁻⁴, 2.2 × 10⁻⁴, 1.6 and 1.6 μg/mL, respectively [50]. Cyclo-(D-8-acetoxyl-Pro-L-Leu) (42) was a novel DKP isolated from Treptomyces sp. SCSIO 41400, which was obtained from a mangrove derived-soil from the Fuli Mangrove Bay Wetland Park, Haikou, Hainan Province of China. Compound 42 could anchor in the binding site of the pancreatic lipase (PL) enzyme and then prevent the substrate from entering and inhibit the PL enzyme activity with an IC₅₀ value of 27.3 μg/mL [51]. All 25 DKPs from the actinomycetes described above are presented in Figure 2.

2.4. Fungi

2.4.1. Fungi from Sediment Origin

Graphiumins A–J (43–52), ten new sulfur-containing DKPs, were isolated from the fungus Graphium sp. OPMF00224 collected from a depth of 17 m marine sediment on Ishigaki Island, Okinawa, Japan. Compounds 45–47 and 49–52 exhibited no inhibition for MRSA; however, they could inhibit the production of the yellow pigment (virulence factors of MRSA) with a white zone of 10, 14, 10, 12, 11, 24 and 23 mm (50 μg/8 mm) [52,53]. Gu et al. firstly utilized the high-speed countercurrent chromatography (HSCCC) to separate and purify the marine fungus secondary metabolites and then two new sulfur-containing DKPs named cladosporins A (53) and B (54) were discovered from the marine fungus Cladosporium sp. collected from marine sediment in Yangshashan Bay, Ningbo, Zhejiang Province, China. Compounds 53 and 54 showed cytotoxicity activities against HepG2 (hepatocellular carcinoma) cell lines with the IC₅₀ values of 21 and 48 μg/mL, respectively. The marine fungus Alternaria alternate HK-25 was isolated from mangrove sediment (Sanya, Hainan, China). By using HSCCC, five DKPs named 12,13-dihydroxy-fumitremorgin C (55), gliotoxin (56), demethoxyfumitremorgin C (57), bisdethiobis(methylthio)gliotoxin (58) and fumitremorgin C (59) were isolated from A. alternate HK-25 and first discovered from fungi. Purities of these compounds were all above 94% [54,55].

Haenamindole (60) was discovered from the fungus Penicillium sp. KCB12F005 isolated from a marine sediment on the coast of Haenam, Korea. Compound 60 exhibited weak inhibitory activity against hepatitis C virus with the IC₅₀ value of 76.3 μM [56,57]. Dichotocejpins A–C (61–63), three novel DKPs, were isolated from the fungus Dichotomomyces cejpii FS110 collected from a depth of 3941 m sediment in the South China Sea. Compound 61 exhibited weak cytotoxicity against SF-268 (human glioma), MCF-7 and HepG2 tumor cell lines with IC₅₀ values of 35.7, 29.5 and 28.9 μM, respectively, and showed remarkably more α-glucosidase inhibition activity than acarbose (oral antidiabetic agent, IC₅₀ = 463 μM) with the IC₅₀ value of 138 μM [58]. Cristazine (64) was discovered from the fungus Chaetomium cristatum, which was isolated from a marine mudflat on Suncheon Bay, Korea. Compound 64 exhibited antioxidant activity against 2,2-diphenyl-1-picrylhydrazyl (DPPH) with the IC₅₀ value of 19 μM, which was similar to ascorbic acid (positive control, IC₅₀ = 20 μM). It also possessed potent cytotoxicity activities against Hela (cervical carcinoma) and A431 (epidermoid carcinoma) cell lines with the IC₅₀ value of 0.5 μM. Compound 64 could trigger the death of the apoptotic cell via the Type I death receptor pathway and inhibit the cell cycle progression by arresting the G₁/S phase and upregulating the inhibitory proteins of cyclin-dependent kinases. By these two ways, compound 64 could inhibit the growth of A431 cells [59,60].

Nineteen novel DKPs namely eutypellazines A–S (65–83) were isolated from fungus Eutypella sp. MCCC 3A00281, which was collected from a depth of 5610 m of marine sediment from the South Atlantic Ocean. Compounds 65–76 could inhibit the replication of human immunodeficiency virus type 1 (HIV-1) with the IC₅₀ values ranging 3.2–18.2 μM. A sulfide bridge existing in compounds 65–67 and 71–72 (IC₅₀ = 10.7–18.2 μM) might be the reason for exhibiting lower inhibitory activities than compounds 68–69 and 73–76 (IC₅₀ = 3.2–8.7 μM). Compound 74 exhibited the ability to reactivate the latent HIV-1 transcription, which was rarely discovered from nature products. These compounds may become new anti-HIV candidates via modifying the original new scaffolds [61,62]. Fusaperazine F (84) was isolated from the fungus Penicillium crustosum HDN153086 collected from the Prydz Bay sediment of the Antarctic and showed cytotoxicity activity against K562 cell lines (human chronic myelogenous leukemia cells, IC₅₀ = 12.7 μM) [63]. Roquefortine J (85) was another new DKP discovered from the fungus Penicillium granulatum MCCC 3A00475 isolated from a -2284 m deep-sea sediment from the Prydz Bay of Antarctica and exhibited moderate cytotoxicity activity against HepG2 cell lines with an IC₅₀ value of 19.5 μM [64,65]. Three pairs of new DKPs namely (±)-7,8-epoxy-brevianamide Q (86–87), (±)-8-hydroxy-brevianamide R (88–89) and (±)-8-epihydroxy-brevianamide R (90–91) were isolated from the fungus Aspergillus versicolor MF180151, which was collected from a sediment in Bohai Sea, China. However, these compounds showed no antimicrobial activities [66].

Raistrickindole A (92), a novel DKP containing indole tetraheterocyclic ring system, was discovered from the fungus Penicillium raistrickii IMB17-034 obtained from a mangrove swamp sediment in Sanya, Hainan Province, China. Compound 92 exhibited the inhibitory activity against hepatitis C virus (HCV) with the EC₅₀ value of 5.7 μM [67]. 5′-hydroxy-6′-ene-epicoccin G (93), 7-methoxy-7′-hydroxyepicoccin G (94), 8′-acetoxyepicoccin D (95), 7′-demethoxyrostratin C (96) and (±)-5-hydroxydiphenylalazines A (97–98) were five new DKPs and isolated from the fungus Epicoccum nigrum SD-388, which was collected from a depth of 4500 m of sediment in the West Pacific. Compound 96 possessed the excellent cytotoxicity activity against Huh7.5 (liver tumor) cell lines with an IC₅₀ value of 9.52 μM, however, it also inhibited the growth of human normal liver LO2 cell lines. The disulfide bridge in the main structure may be crucial for its activity [68]. (±) Eurotinoids A (99–100), (±) eurotinoids B (101–102) and (±) eurotinoids C (103–104), three pairs of novel DKPs spirocyclic alkaloid enantiomers, were isolated from the fungus Eurotium sp. SCSIO F452 obtained from the sediment sample in South China Sea. These compounds showed excellent antioxidant activities against DPPH with the IC₅₀ values between 5.8 and 24.9 μM [69].

Seven novel DKPs, versicamides A−G (105−111), were isolated from the fungus Aspergillus versicolor HDN08-60 collected from the marine sediment in the South China Sea. Versicamide H (112) was obtained from the methylation reaction of 111. Only compound 112 showed modest cytotoxicity activities against the HL-60, HCT-116, Hela and K562 cell lines with IC₅₀ values of 8.7, 17.7, 19.4 and 22.4 μM, respectively, and compound 112 exhibited selective PTK inhibitory activity with the highest inhibitory rate of 60% at a concentration of 10 μM. Compound 112 had stronger activities than compounds 105–111, which might be attributed to an unprecedented skeleton featuring a 2,5-dihydro-1H-azepino-[4,3-b]quinoline system [70]. Fifteen novel DKPs namely rubrumlines A–O (113–127) were isolated from the fungus Eurotium rubrum, which was collected from a depth of 2067 m marine sediment in the South Atlantic Ocean. Compound 116 possessed antivirus activity against the influenza A/WSN/33 virus with the inhibitory rate of 52.64% [71]. Ten new DKPs, 12β-hydroxy-13α-ethoxyverruculogen TR-2 (128), 12β-hydroxy-13α-butoxyethoxyverruculogen TR-2 (129), hydrocycloprostatin A (130), hydrocycloprostatin B (131), 25-hydroxyfumitremorgin B (132), 12β-hydroxy-13α-butoxyethoxyfumitremorgin B (133), 12β-hydroxy-13α-methoxyverruculogen (134), 26α-hydroxyfumitremorgin A (135), 25-hydroxyfumitremorgin A (136) and diprostatin A (137) were isolated from the coculture of the fungus Penicillium sp. DT-F29 with the bacteria Bacillus sp. B31, which were collected from the marine sediments of Dongtou County and Changzhi Island, respectively. In a dose of 20 μM, compounds 133 and 137 showed remarkable BRD4 protein inhibitory activities [72].

Three novel pairs of spirocyclic DKPs namely (±) variecolortins A−C (138−143) were obtained from the fungus Eurotium sp. SCSIO F452, which was isolated from marine sediment in the South China Sea. Compound 138 had a 2-oxa-7-azabicyclo[3.2.1]octane core, which was an unprecedented highly functionalized seco-anthronopyranoid carbon skeleton. In addition, compounds 139 and 140 had a rare 6/6/6/6 tetracyclic cyclohexene−anthrone carbon scaffold. Compound 138 showed remarkable antioxidant activity against DPPH with an IC₅₀ value of 58.4 μM. Compound 140 and 142 exhibited modest cytotoxicity activities against SF-268 (IC₅₀ = 12.5 and 15.0 μM) and HepG2 (IC₅₀ = 30.1 and 37.3 μM) cell lines. The preliminary molecular docking study revealed that the potential antioxidative target of compounds 138 and 139 might be peroxiredoxin and the potential cytotoxic target of compounds 140 and 142 might be farnesyltransferase [73]. Eurotiumins A–C (144–146), three new DKP alkaloids, were also isolated from the fungus Eurotium sp. SCSIO F452. Compounds 144–146 showed antioxidant activities against DPPH with IC₅₀ values of 37, 69 and 13 μM, respectively. Based on these results, the absolute configurations of the C-2 and C-3 of compounds 144 and 145 might have an influence on antioxidant activities [74]. All 104 DKPs from sediment-derived fungi described above are presented in Figure 3, Figure 4 and Figure 5.

2.4.2. Fungi from Sponge Origin

A novel DKP namely 6-acetylmonodethiogliotoxin (147) was obtained from the fungus Dichotomomyces cejpii, which was collected from the marine sponge Callyspongia cf. C. flammea at Bare Island, Sydney, Australia. Two DKPs previously only known as semisynthetic compounds, 6-acetylbisdethiobis(methylthio)gliotoxin (148) and 5a,6-anhydrobisdethiobis(methylthio)gliotoxin (149), were found in nature for the first time. In human chronic myeloid leukemia cells, compounds 147 and 148 showed downregulated TNFα-induced NF-κB activity with the IC₅₀ values of 38.5 and 65.7 μM, respectively [75]. Peniciadametizine A (150) and B (151) were isolated from the fungus Penicillium adametzioides AS-53 collected from an unidentified sponge in Hainan Island of China. Compound 150 had a unique spiro[furan-2,7’-pyrazino[1,2-b][1,2]oxazine] skeleton, which was found from a natural source for the first time and compound 151 was the highly oxygenated analogue of 150. In a dose of 100 μg/mL, compounds 150 and 151 could kill Artemia salina with the lethal ratio of 45.5 and 62.4%, respectively. These two compounds also showed antifungal activity against Alternaria brassicae with MIC values of 4.0 and 32.0 μg/mL, respectively [76]. Cyclo-(2-hydroxy-Pro-Gly) (152), a novel minor DKP, was obtained from the fungus Simplicillium sp. YZ-11, which was collected from an intertidal sponge Hymeniacidon perleve from Dalian, Liaoning Province, China [77]. Neosartorya glabra KUFA 0702 was isolated from the sponge Mycale sp. collected from 15 to 20 m coral reef at Samaesarn Island and a new DKP, fellutanine A (153), was obtained. Compound 153 showed no antibacterial (MIC > 256 μg/mL) or antifungal (MIC > 512 μg/mL) activity against the tested microbe including E. coli ATCC 25922, Staphylococcus aureus ATCC 25923, A. fumigatus ATCC 46645, Trichophyton rubrum ATCC FF5 and C. albicans ATCC 10231 [78]. Asperflocin (154), a novel asymmetric DKP dimer, was isolated from the fungus Aspergillus versicolor 16F-11, which was obtained from the sponge Phakellia fusca from Yongxing Island in the South China Sea. Compound 154 could inhibit the A375 (human melanoma) cell lines growth with the IC₅₀ value of 10.29 ± 2.37 μM [79].

One novel DKP dimer (155) possessing the same planar structure and different stereochemistry with an unnamed and ambiguous compound was isolated from the fungus Aspergillus violaceofuscus, which was collected from sponge Reniochalina sp. from Xisha Islands in the South China Sea. In a dose of 10 μM, compound 155 exhibited anti-inflammatory activity via decreasing LPS-induced expression of IL-10 in THP-1 cells with inhibitory rates of 78.1% [80]. Waspergillamide B (156), a new DKP containing an unusual p-nitrobenzoic acid structure, was obtained from the fungus Aspergillus ochraceus collected from the sponge Agelas oroides from the Mediterranean. Compound 156 had no cytotoxic activity against A2780 (human ovarian carcinoma) cell lines [81]. One new DKP alkaloid namely penicillivinacine (157) was obtained from the fungus Penicillium vinaceum that was isolated from the marine sponge Hyrtios erectus from Yanbu. Compound 157 exhibited significant cytotoxicity activity against MDA-MB-231 cell lines with an IC₅₀ value of 18.4 μM [82]. Adametizines A (158) and B (159), two novel sulfur-containing DKPs, were separated from the fungus Penicillium adametzioides AS-53, which was isolated from an unidentified marine sponge collected from Hainan Island of China. Compound 158 could inhibit the growth of brine shrimp (A. salina) with the LD₅₀ (lethal dose 50%) value of 4.8 μM. In addition, compound 158 showed antimicrobial activities against Staphylococcus aureus, Aeromonas hydrophilia and V. parahaemolyticus with an MIC value of 8 μg/mL and compound 159 showed antimicrobial activities against S. aureus with an MIC value of 64 μg/mL. These results revealed that the Cl substitution at C-7 remarkably increased the brine shrimp lethality and antimicrobial activity [83]. SF5280-415 (160) was a novel DKP dimer and isolated from the fungus Aspergillus sp. SF-5280, which was obtained from an unidentified sponge at Cheju Island, Korea. Compound 160 showed enzyme inhibitory activity against the PTP1B enzyme with an IC₅₀ value of 14.2 μM [84]. All 14 DKPs from sponge-derived fungi described above are presented in Figure 5.

2.4.3. Fungi from Beach Origin

A novel DKP namely penicimutide (161) was obtained from a neomycin-resistant mutant fungus Penicillium purpurogenum G59, which was isolated from a soil sample from the tideland of Bohai Bay. In a dose of 100 μg/mL, compound 161 exhibited excellent cytotoxicity activity against HeLa cell lines with an inhibition rate of 39.4% [85]. Penicimutanin C (162), a new DKP contained alkaloidal, was isolated from the neomycin-resistant mutant strain 3-f-31 fungus P. purpurogenum G59. Compound 162 exhibited cytotoxic activities against HeLa, BGC-823 (gastric adenocarcinoma), MCF-7, K562 and HL-60 (acute promyelocytic leukemia) cell lines with IC₅₀ values of 11.9, 5.0, 8.6, 8.7 and 6.0 μM, respectively. In a dose of 100 μg/mL, compound 162 showed cytotoxicity activities against these five cell lines with inhibition rates of 88.1%, 83.9%, 80.5%, 87.7% and 87.3%, respectively [86].

Waikikiamides A−C (163–165) were obtained from the fungus Aspergillus sp. FM242 collected from a sample at Waikiki beach in Oahu, Honolulu, Hawaii. Compounds 163 and 164 contained a new skeleton with a hendecacyclic ring system and compound 165 was composed of two notoamide analogs with an N-O-C bridge to feature the unique heterodimer. Compounds 163 possessed antiproliferative activities against HT1080 (fibrosarcoma), PC3 (prostatic tumor), Jurkat (immortalized T lymphocyte) and A2780S cell lines with IC₅₀ values of 0.519, 1.855, 0.62 and 0.78 μM, respectively. Compound 165 exhibited antiproliferative activities against these four cell lines with IC₅₀ values of 1.135, 1.805, 1.79 and 1.127 μM, respectively. Compound 164 showed no activities to these four cell lines. The reason for the difference in activity was that compounds 163 and 165 possessed an N–O bond but compound 164 did not [87]. All 5 DKPs from fungi of beach origin described above are presented in Figure 5.

2.4.4. Fungi from Mangrove Origin

A new DKP namely 5S-hydroxynorvaline-S-Ile (166), together with two firstly discovered in nature namely 3S-hydroxylcyclo(S-Pro-S-Phe) (167) and cyclo(S-Phe-S-Gln) (168), were obtained from mangrove endophytic fungus Penicillium sp. GD6, which was isolated from the stem bark of Bruguiera gymnorrhiza collected from Zhanjiang, China. These compounds showed no activity against the tested MRSA [88]. (−)-asperginulin A (169) and (+)-asperginulin A (170), two dimers DKPs that contained enantiomeric indole, were isolated from the mangrove endophytic fungus Aspergillus sp. SK-28, which was obtained from Kandelia candel from the Shankou Mangrove Nature Reserve in Guangxi Province, China. Compound 170 could inhibit the growth of the barnacle Balanus reticulatus with antifouling activity and low toxicity [89]. Three novel DKPs, saroclazines A–C (171–173), were isolated from the fungus Sarocladium kiliense HDN11-84, which was obtained from a root soil sample of mangrove Thespesia populnea from Guangxi Province, China. Compound 171 exhibited cytotoxicity activity against Hela cell lines with the IC₅₀ value of 4.2 μM [90].

Fifteen novel DKPs, brocazines A−F (174−179), penicibrocazines A–E (180–184), spirobrocazines A−C (185−187) and brocazine G (188) were obtained from the fungus Penicillium brocae MA-231, which was isolated from the marine mangrove plant Avicennia marina in Hainan Island. Compounds 174–177 showed potent cytotoxic activities against Du145 (human prostate carcinoma), Hela, HepG2, MCF-7, NCI-H460 (human non-small cell lung cancer), SGC-7901 (human gastric carcinoma), SW1990 (human pancreatic adenocarcinoma) and U251 cell lines with the IC₅₀ values in the range of 0.89–12.4 μM [91]. Compounds 182 and 184 exhibited antimicrobial activities against S. aureus and Gaeumannomyces graminis with the MIC values ranging from 0.25 to 32.0 μg/mL, respectively, and compound 183 also showed antimicrobial activities against S. aureus and Micrococcus luteus with the MIC values of 0.25 μg/mL [92]. In addition, compounds 185 and 187 showed modest antimicrobial activities against E. coli and Vibrio harveyi with the MIC values in the range of 32–64 μg/mL. Compound 188 exhibited significant cytotoxic activities against A2780 and A2780 CisR cell lines with the IC₅₀ values of 664 and 661 nM, respectively, and had potent antimicrobial activity against S. aureus with an MIC value of 0.25 μg/mL [91,93]. These results showed that compounds possessing two double bonds at C-6 and C-6′ or one double bond at C-6/6′ conjugating with a keto group at C-5/5′ might exhibit higher cytotoxic or antimicrobial activity. All 23 DKPs from mangrove-derived fungi described above are presented in Figure 6.

2.4.5. Fungi from Coral Origin

Pseudellones A (189) and B (190), two new DKPs containing an irregular bridge, were separated from the fungus Pseudallescheria ellipsoidea F42−3 derived from the soft coral Lobophytum crassum in Hainan Sanya National Coral Reef Reserve, China [94]. Pseuboydones C (191) and D (192) were isolated from the fungus Pseudallescheria boydii F19-1 collected from the soft coral L. crassum. Compound 191 exhibited remarkable cytotoxic activity against Sf9 cell lines with an IC₅₀ value of 0.7 μM [95]. Fungus Dichotomomyces cejpii F31-1 was collected from the soft coral L. crassum and two novel DKPs namely dichocerazines A (193) and B (194) were then obtained from the GPY medium (added L-tryptophan and L-phenylalanine) of the fungus. These two compounds showed no activity for the tested HCT116, RD (human rhabdomyosarcoma), ACHN (human renal carcinoma) and A2780T cell lines [96]. Three new DKPs alkaloids namely 11-methylneoechinulin E (195), variecolorin M (196) and (+)-variecolorin G (197) and a DKP first discovered in nature namely (+)-neoechinulin A (198) were obtained from the fungus Aspergillus sp. EGF 15-0-3, which was isolated from a soft coral in the South China Sea. Compounds 195–198 showed cytotoxic activity against NCI-H1975 gefitinib resistance cell lines at the concentration of 50 μM [97].

16α-hydroxy-17β-methoxy-deoxydihydroisoaustamide (199), 16β-hydroxy-17α-methoxy-deoxydihydroisoaustamide (200), 16β,17α -dihydroxy-deoxydihydroisoaustamide (201), 16α -hydroxy-17α-methoxy-deoxydihydroisoaustamide (202), 16α,17α -dihydroxy-deoxydihydroisoaustamide (203), 16,17-dihydroxy-deoxydihydroisoaustamide (204) and 3β-hydroxy-deoxyisoaustamide (205), seven new DKPs containing a prenylated indole ring, were obtained from the fungus Penicillium dimorphosporum KMM 4689, which was separated from an unidentified soft coral in the South China Sea. When the murine neuroblastoma Neuro-2a cells were treated with the mixture of 500 μM paraquat (PQ) and 1 μM each of compounds 202, 203 and 204, the cell viability was increased by 38.6%, 30.3% and 36.5%, respectively, compared with the treatment of PQ alone. The hydroxy groups at C-16 and C-17 played a key role in neuroprotective activity by the analysis of structure–activity relationships [98]. Pseudellone D (206), a novel DKP alkaloid possessing a rare monomethylthio group, was isolated from the fungus Pseudallescheria ellipsoidea F42-3 sourced from the soft coral Lobophytum crissum [99]. All 18 DKPs from coral-derived fungi described above are presented in Figure 6.

2.4.6. Fungi from Alga Origin

Dehydroxymethylbis(dethio)bis(methylthio)gliotoxin (207) and (3S,6R)-6-(para-hydroxybenzyl)-1,4-dimethyl-3,6-bis(methylthio)piperazine-2,5-dione (208), two novel sulphurated DKPs, were obtained from the fungus Trichoderma virens Y13-3 derived from the marine red alga Gracilaria vermiculophylla in Yangma Island [100]. Methylcordysinin A (209) was separated from Trichoderma asperellum cf44-2, which was collected from brown alga Sargassum sp. in Zhoushan Islands [101]. Four novel DKP alkaloids named citriperazines A-D (210–213) were separated from Penicillium sp. KMM 4672 obtained from the Vietnamese marine brown algae Padina sp. [102]. Compounds 207–213 showed no inhibitory activities for the tested bacteria or cancer cell lines. The endophytic fungus Acrostalagmus luteoalbus TK-43 was isolated from the green algal Codium fragile collected in Sinop, Turkey, and six novel N-methoxy-containing indole DKPs, namely (±) acrozines A–C (214–219), were obtained from the strain. Compound 216 exhibited moderate antimicrobial activity against the plant pathogen Fusarium solani (MIC = 32 μg/mL). Compounds 214 and 215 possessed antiacetylcholinesterase activities with the IC₅₀ values of 2.3 and 13.8 μM, respectively. This result indicated that the bioactivity was concerned with the absolute configurations of these compounds [103].

One new DKP namely cyclo(L-5-MeO-Pro-L-5-MeO-Pro) (220) was isolated from the fungus Trichoderma asperellum A-YMD-9-2, which was obtained from marine macroalga Gracilaria verrucose collected from Yangma Island. Compound 220 exhibited inhibitory activities against Chattonella marina, Heterosigma akashiwo, Karlodinium veneficum and Prorocentrum donghaiense with the EC₅₀ values of 47.3, 276, 327 and 351 μM, respectively [104]. Three novel sulfur-containing DKPs namely pretrichodermamides D–F (221–223) were separated from the fungus Penicillium sp. KMM 4672, which was obtained from the Vietnamese brown alga Padina sp. These compounds did not show potent activities for the human prostate cancer 22Rv1 cells [105]. Four new DKP alkaloids namely N-(4′-hydroxyprenyl)-cyclo(alanyltryptophyl) (224), isovariecolorin I (225), 30-hydroxyechinulin (226) and 29-hydroxyechinulin (227) were obtained from the fungus Eurotium cristatum EN-220, which was collected from marine alga Sargassum thunbergia on the coast of Qingdao, China. Compound 225 showed brine shrimp (A. salina) lethal activity with the LD₅₀ value of 19.4 μg/mL and had moderate antioxidative activities with an IC₅₀ value of 20.6 μg/mL [106]. (±)-Brevianamides X (228 and 229) were obtained from the fungus Aspergillus versicolor OUCMDZ-2738 isolated from alga Enteromorpha prolifera, which was collected from Shilaoren beach, Qingdao, China, and showed no antimicrobial activities [107]. All 23 DKPs from alga-derived fungi described above are presented in Figure 7.

2.4.7. Fungi from Other Origin

One novel DKP namely isoechinulin D (230) was isolated from the marine fungus Eurotium rubrum MPUC136, which was collected from the seaweed in Chosei-mura, Chosei-gun, Chiba Prefecture, Japan, and showed weak inhibitory activity against melanin synthesis with an IC₅₀ value of 60 μM. [108]. Spirotryprostatin G (231), cyclotryprostatins F (232) and G (233), three new DKPs alkaloids, were isolated from the fungus Penicillium brasilianum HBU-136 separated from the Bohai Sea. Compound 231 showed excellent cytotoxic activity against HL-60 cell lines with an IC₅₀ value of 6.0 μM and compounds 232 and 233 exhibited remarkable cytotoxic activity against MCF-7 cell lines with IC₅₀ values of 7.6 and 10.8 μM, respectively [109]. A new DKP alkaloid namely penilline C (234) was obtained from the fungus Penicillium chrysogenum SCSIO 07007, which was isolated from a deep-sea hydrothermal vent environment sample of Western Atlantic [110]. Emestrins L (235) and M (236), two novel DKPs, were separated from the fungus Aspergillus terreus RA2905, which was obtained from the sea hare Aplysia pulmonica from the Weizhou coral reefs in the South China Sea. Compound 236 showed antifungal activity against P. aeruginosa ATCC 27853 with the MIC value of 64 μg/mL [111].

Aspamides A–D (237–240), four novel DKPs alkaloids, were separated from the endophyte fungus Aspergillus versicolor DY180635, which was isolated from the sea crab Chiromantes haematocheir from the intertidal zone of Zhoushan, Zhejiang, China. For the virtual screening on the 3CL hydrolase (Mpro) of SARS-CoV-2 (potential drug target to fight COVID-19), the docking scores of compounds 237 and 238 were −5.389 and −4.772, respectively, and the score of positive control ritonavir was −7.039. In the future, these two compounds may be helpful in fighting COVID-19 [112]. One novel DKP namely penicillatide B (241) was isolated from the fungus Penicillium sp., which was collected from the Red Sea tunicate Didemnum sp. Compound 241 showed moderate cytotoxic activity against the HCT-116 cell lines with an IC₅₀ value of 23.0 μM and exhibited modest antimicrobial activities against S. aureus and V. anguillarum with inhibition zones of 19 and 20 mm, respectively [113]. All 12 DKPs from fungi sourced from other origins described above are presented in Figure 7.

3. Chemical Structures of Diketopiperazine Derivatives from Marine Organisms

Diketopiperazine derivatives are further modified on the basis of the six-membered piperazine rigid backbone. The following 54 DKP derivatives were described in this paper, of which 53 lacked a carbonyl group and 1 lacked two carbonyl groups in the skeleton (Table 2).

3.1. Actinomycetes

A mixture of two new tautomers DKP derivatives named isomethoxyneihumicin (242 and 243) were obtained from the actinomycete Nocardiopsis alba KM6-1, which was collected from marine sediment in Chichijima, Ogasawara, Japan. The mixture showed excellent cytotoxic activity against Jurkat cell lines with the IC₅₀ value of 6.98 μM and in a dose of 15 μM, compounds 242 and 243 made the cell cycle of Jurkat cell lines staying in the G2/M phase with the inhibition ratio of 66% in 12 h. These consequences indicated that the mixed compounds inhibited the growth of Jurkat cell lines via arresting the cell cycle at the G2/M phase [114]. Two novel DKP derivatives namely nocazines A (244) and B (245) were isolated from Nocardiopsis dassonvillei HR10-5, which was obtained from marine sediment in the estuary of Yellow River, Dongying, China. However, compounds 244 and 245 did not exhibit cytotoxic or antimicrobial activities for the tested cancer cell lines and microorganisms [115].

Nocazines F (246) and G (247), two novel DKP derivatives, were isolated from the Nocardiopsis sp. YIM M13066, which was collected from the deep-sea sediment. Compound 246 showed remarkable cytotoxic activities against the human cancer cell lines H1299 (non-small cell lung cancer), Hela, HL7702, MCF-7, PC3 and U251 with IC₅₀ values of 3.87, 4.77, 7.10, 3.86 and 8.17 μM, respectively. Compound 247 also showed excellent cytotoxic activities against the human cancer cell lines H1299, Hela, HL7702 (human derived liver), MCF-7, PC3 and U251 with the IC₅₀ values of 2.60, 3.97, 8.73, 6.67 and 16.7 μM, respectively, and exhibited modest antimicrobial activity against B. subtilis ATCC 6051 with the MIC value of 25.8 μM [116]. Streptopyrazinones A–D (248–251) were four new DKP derivatives and isolated from Streptomyces sp. ZZ446, which was collected from a coastal soil sample from Zhoushan Islands. Compounds 248–251 exhibited moderate antimicrobial activities against C. albicans and MRSA with the MIC values of 35–45 and 58–65 μg/mL, respectively [44]. One novel tricyclic DKP derivative namely strepyrazinone (252) was obtained from Streptomyces sp. B223, which was isolated from the marine sediment of Laizhou Bay. Compound 252 displayed remarkable cytotoxic activity against the HCT-116 cell lines with the IC₅₀ value of 0.34 μM [117]. All 11 DKP derivatives from actinomycetes described above are presented in Figure 8.

3.2. Fungi

Varioloids A (253) and B (254) were two novel oxepine-containing DKP derivatives and obtained from the endophytic fungus Paecilomyces variotii EN-291, which was collected from red alga Grateloupia turuturu on the coast of Qingdao. Compounds 253 and 254 showed significant antimicrobial activities against F. graminearum with the MIC values of 8 and 4 μg/mL, respectively. In addition, compounds 253 and 254 also inhibited the growth of A. hydrophila, E. coli, M. luteus, S. aureus, V. anguillarum, V. harveyi and V. parahaemolyticus with the MIC values in the range of 16–64 μg/mL [118]. Four novel oxepine-containing DKP derivatives namely oxepinamides H−K (255–258) and four novel 4-quinazolinone DKP derivatives namely puniceloids A−D (259–262) were isolated from the fungus Aspergillus puniceus SCSIO z021, which was collected from deep-sea sediment in Okinawa Trough. Compounds 255–257 and 259–262 exhibited remarkable transcriptional activation of liver X receptor α with the EC₅₀ values of 1.7–16 μM. This result suggested that the transcriptional activation activity of these compounds would be decreased when the benzene ring was converted into an oxepin unit. In addition, compound 262 possessed enzyme inhibitory activities against seven enzymes including TCPTP, SHP1, MEG2, SHP2, PTP1B, IDO1 and LDHA with the IC₅₀ values in the range of 14–87 μM [119].

Protuboxepins C (263) and D (264), two novel oxepin-containing DKP derivatives, were obtained from the fungus Aspergillus sp. SCSIO XWS02F40, which was isolated from the sponge Callyspongia sp. from the sea area near Xuwen County, Guangdong Province, China. Compounds 263 and 264 showed moderate cytotoxic activities against Hela cell lines with the IC₅₀ values of 61 and 114 μM, respectively [120,121]. Pyranamides A−D (265–268), secopyranamide C (269) and protuboxepins F−J (270–274), ten novel DKP derivatives, were isolated from the marine sponge-derived fungus Aspergillus versicolor SCSIO 41016, which was also separated from Callyspongia sp. Compound 270 showed modest cytotoxic activities against the ACHN, OS-RC-2 and 786-O cell lines (three renal carcinoma cell lines) with the IC₅₀ values of 27, 34.9 and 47.1 μM, respectively [122]. Chrysopiperazines A–C (275–277) were three new DKP derivatives and obtained from the fungus Penicillium chrysogenum, which was collected from gorgonian Dichotella gemmacea in South China Sea. The oxepine-containing DKPs were found from the genus Penicillium for the first time [123]. One novel DKP derivative namely quinadoline D (278) was isolated from the fungus Penicillium sp. L129, which was collected from the rhizosphere-soil of Limonium sinense (Girald) Kuntze from Yangkou Beach, Qingdao, China [124]. Two novel DKP derivatives namely aspamides F (279) and G (280) were isolated from the endophyte fungus A. versicolor DY180635. For the virtual screening on the 3CL hydrolase of SARS-CoV-2, the docking scores of compounds 279 and 280 were −5.146 and −4.962, respectively [112].

Polonimides A–C (281–283), three novel quinazoline-containing DKP derivatives, were isolated from the fungus Penicillium polonicum obtained from the Bohai Sea. Compounds 281–283 showed potent chitinase inhibitory activity against GH18 chitinase OfChi-h with the inhibition rates of 91.9%, 79.1% and 86.1%, respectively [125]. Protuboxepin K (284) was obtained from the fungus Aspergillus sp. BFM-0085, which was collected from a marine sediment sample of Tokyo Bay. In mutant bone morphogenetic protein (BMP) receptor-carrying C2C12 (R206H) cells, compound 284 exhibited the BMP-induced alkaline phosphatase inhibitory activity with the IC₅₀ value of 4.7 μM [126]. One novel oxepine-containing DPK derivative namely varioxepine B (285) was isolated from the fungus Aspergillus terreus, which was collected from soft coral Sarcophyton subviride on Xisha Island. Compound 285 showed excellent inhibitory activity against Con A-induced murine splenocytes with the inhibition rates of 20%, 28%, 23% and 80% at the concentration of 64, 128, 256 and 512 nM, respectively, and had no effect on cell viability at the concentration of 100 μM. Meanwhile, compound 285 also remarkably decreased the cytokine (interferon-γ, interleukin-2 and tumor necrosis factor-α) production by activating murine splenocytes. Furthermore, compound 285 showed significant inhibitory activity against anti-CD3/anti-CD28 mAb-induced murine splenocytes, human T cell proliferation and Th1/Th2 cytokine production [127].

Three novel DKP derivatives namely 3-hydroxyprotuboxepin K (286), 3,15-dehydroprotuboxepin K (287) and versiamide A (288) were isolated from the fungus Aspergillus creber EN-602 derived from marine red alga Rhodomela confervoides on the coast of Qingdao, China. Compound 286 showed enzyme inhibitory activity against the angiotensin converting enzyme with the IC₅₀ value of 22.4 μM. In addition, compound 287 exhibited antimicrobial activities against Edwardsiella tarda, E. coli, M. luteus, P. aeruginosa and V. harveyi with the MIC values in the range of 8–64 μg/mL and compound 288 exhibited antimicrobial activities against A. hydrophila, E. coli, M. luteus and P. aeruginosa with the MIC values between 16 and 64 μg/mL [128]. Protuboxepins A (289) and B (290) were two novel oxepin-containing DKP derivatives and were isolated from the fungus Aspergillus sp. SF-5044, which was collected from the intertidal sediment from Dadaepo Beach, Busan, Korea. Compound 289 exhibited weak cytotoxic activities against HL-60, MDA-MB-231, Hep3B (human liver carcinoma), 3Y1 and K562 cell lines with the IC₅₀ values of 75, 130, 150, 180 and 250 μM, respectively. Compound 289 possessed a disrupting microtubule dynamics ability and induced apoptosis in cancer because it could bind to α,β-tubulin and stabilize tubulin polymerization and then leading to chromosome misalignment and metaphase arrest in cancer [129,130].

Carnequinazolines A–C (291–293), three novel DKP derivatives, were separated from the fungus Aspergillus carneus KMM 4638 collected from the marine brown alga Laminaria sachalinensis, which was isolated from Kunachir Island. Compounds 291 and 292 had no cytotoxicity and antimicrobial activities [131]. One novel alkaloid DKP derivative namely fumiquinazoline K (294) was obtained from the fungus Aspergillus fumigatus KMM 4631, which was separated from soft coral Sinularia sp. in Kuril islands, and showed no enzyme inhibition and cytotoxic activities [132]. 3-[6-(2-Methylpropyl)-2-oxo-1H-pyrazin-3-yl]propanamide (295) was obtained from the fungus Aspergillus versicolor OUCMDZ-2738, and exhibited no antimicrobial and α -glucosidase inhibitory activity [107]. All 43 DKP derivatives from fungi described above are presented in Figure 8.

4. Characteristics of Bioactive Diketopiperazines and Their Derivatives from Marine Organisms

In this review, 241 DKPs and 54 DKP derivatives isolated from marine organisms were summarized, among which fungi and actinomycetes were the most abundant sources. These marine organisms come from a wide range of sources and the red dots (Figure 9) and yellow dots (Figure 10) represent the collection points for marine biological samples, which produced DKPs and DKP derivatives, respectively. DKPs and DKP derivatives of fungi sources were 199 (82.6%) and 43 (79.6%), respectively, and those of actinomycetes sources were 25 (10.4%) and 11 (20.4%), respectively (Figure 11a,b). In addition, DKPs of sponge and bacteria sources were 5 (2.1%) and 12 (5%), respectively (Figure 11a).

These DKPs had antimicrobial (20, 18.3%), cytotoxic (39, 35.8%), enzyme inhibition (5, 4.6%), antiviral (14, 12.8%), antioxidant (11, 10.1%) and other activities (20, 18.3%) (Figure 11c). Furthermore, these DKP derivatives also had antimicrobial (9, 30%), cytotoxicity (9, 30%), enzyme inhibition (5, 16.7%) activities and a possessed transcriptional activation (7, 23.3%) effect (Figure 11d). Subtle differences in chemical structures are closely related to the bioactivity. For example, compound 96 possessing a unique disulfide bridge in the six-membered piperazine skeleton showed much more significant cytotoxic activity than compounds 93–95, 97 and 98. Compounds 163 and 165 exhibited remarkable antiproliferative activities but compound 164 was inactive, which indicated that the N–O bond played an important role for their bioactivity. In addition, the absolute configurations might be closely associated with the activity intensity. For instance, the antioxidant activity of compounds 144 (IC₅₀ = 37 μM) and 145 (IC₅₀ = 69 μM) were relevant to the absolute configurations of C-2 and C-3. Different substituents in the same chemical skeleton may lead to different activities. For example, compound 158 bearing a Cl atom at C-7 exhibited stronger antimicrobial and brine shrimp inhibition activity than compound 159.

5. Conclusions

In recent years, the number of papers and patents related to DKPs is on the rise continuously and many novel DKPs had been isolated from marine sources. More and more researchers are turning their attention to the six-membered ring rigid structure with great potential for biological activity. It is a heterocyclic scaffold with restricted conformation, which can control stereochemistry at up to four positions. These features provide its potential to break the planarity of traditional drugs. Natural DKPs have more interesting structural complexity and biological characteristics and possibly can be further chemically synthesized or modified to increase their activity, promoting the natural product and synthetic chemistry to complement each other. In addition, DKPs can be used as the quorum sensing signal molecule of Shewanella baltica (a kind of unique microorganism produced during transporting the large yellow croaker at 4 °C) and inhibiting the production of DKPs can slow down the spoilage of the large yellow croaker [7]. Furthermore, thaxtomin A has good herbicidal activity and achieves an herbicidal purpose by inhibiting cellulose synthesis [133]. With the tremendous advancement of technology, the known compound can be initially eliminated through the Global Natural Products Social (GNPS) molecular networking project [134]. It would be helpful for targeting directly to discover new natural DKPs and further enrich the library of DKPs.

To sum up, DKPs are potential bioactive chemical substances that are valuable for further exploration from natural sources, especially from marine environments. The conversion of promising bioactive DKPs into clinical drugs for the treatment of diseases needs much more time and energy for researchers. DKPs are regarded as unprocessed diamonds, attracting scientists to take efforts to study their pharmacological properties and therapeutic effects.

Author Contributions

Z.S. wrote the paper, Y.H., Q.Y. and X.L. checked the paper, S.W. verified the content. All authors have read and agreed to the published version of the manuscript.

Funding

This research was funded by the National Natural Science Foundation of China (grant number 81860634), Applied Basic Research Key Project of Yunnan Province (grant number 202001BB050029), Major Science and Technology Projects of Yunnan Province (Digitalization, development and application of biotic resource, grant number 202002AA100007), Project of Innovative Research Team of Yunnan Province (grant number 202005AE160005), and Science Research Foundation of Yunnan Province Education Department (grant number 2021Y041).

Institutional Review Board Statement

Not applicable.

Informed Consent Statement

Not applicable.

Data Availability Statement

All data in this article is openly available without any restrictions.

Conflicts of Interest

The authors declare no conflict of interest.

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Figures and Tables

Figure 1. DKP structures from sponge (1–5) and bacteria (6–17).

Figure 2. DKP structures from actinomycetes (18–42).

Figure 3. DKP structures from sediment-derived fungi (43–83).

Figure 4. DKP structures from sediment-derived fungi (84–127).

Figure 5. DKP structures from sediment-derived (128–145), sponge-derived (146–161) and beach-derived fungi (162–165).

Figure 6. DKP structures from mangrove-derived (166–188) and coral-derived fungi (189–206).

Figure 7. DKP structures from alga-derived (207–229) and other sourced fungi (230–241).

Figure 8. DKP derivative structures from actinomycetes (242–252) and fungi (253–295).

Figure 9. Collection points for marine biological samples producing DKPs (red dots).

Figure 10. Collection points for marine biological samples producing DKP derivatives (yellow dots).

Figure 11. The proportion of DKPs from sponges, bacteria, actinomycetes and fungi (a); the proportion of DKP derivatives from actinomycetes and fungi (b); the proportion of antimicrobial, cytotoxicity, enzyme inhibition, antiviral, antioxidant and other activities of DKPs (c); the proportion of antimicrobial, cytotoxicity, enzyme inhibition and transcriptional activation activities of DKP derivatives (d).

Table 1

The bioactivities, sources and habitats of DKPs during 2014–2021.

Sources	Compounds	Bioactivities	Species	Habitats	Refs
Sponge	Cyclo-(R-Pro-6-hydroxyl-S-Ile) (1)	- a	Callyspongia sp.	South China Sea	[30]
	Geobarrettin A (2)	-	G. barrette	Iceland	[31]
	Geobarrettin B (3)	Anti-inflammatory	G. barrette	Iceland	[32]
	Tedanizaine A (4)	-	Tedania sp.	Guangdong	[32]
	(−)-Cyclo(L-trans-Hyp-L-Ile) (5)	-	A. sinoxea	Larak Island	[33]
Bacteria	Cyclo(Trp-Ser) (6)	Antimicrobial Antiquorum sensing	R. aquimaris QSI02	Yellow Sea	[34]
	Cyclo(Pro-Val) (7)	-	G. antarctica PI12	Antarctica	[35]
	(−)-Cyclo(Pro-Tyr) (8)	-	G. antarctica PI12	Antarctica	[35]
	(−)-Cyclo(Pro-Phe) (9)	-	G. antarctica PI12	Antarctica	[35]
	(+)-Cyclo(Pro-Leu) (10)	-	G. antarctica PI12	Antarctica	[35]
	(3S,6S)-3,6-Diisobutylpiperazine-2,5-dione (11)	Antimicrobial	Bacillus sp. SPB7	S. officinalis	[36]
	Gallaecimonamide A (12)	Antimicrobial	G. mangrovi HK-28	Mangrove sediment	[37]
	Gallaecimonamide A (13)	-	G. mangrovi HK-28	Mangrove sediment	[37]
	Gallaecimonamide A (14)	-	G. mangrovi HK-28	Mangrove sediment	[37]
	cis-Cyclo(Pro-3-chloro-Tyr) (15)	-	B. subtilis BI0980	Kerkyra and Erikoussa	[38]
	trans-Cyclo(Pro-3-chloro-Tyr) (16)	-	B. subtilis BI0980	Kerkyra and Erikoussa	[38]
	cis-Cyclo(3-chloro-Tyr-Ile) (17)	-	B. subtilis BI0980	Kerkyra and Erikoussa	[38]
Actinomycetes	iso-Naseseazine B (18)	Antimicrobial	Streptomyces sp. SMA-1	Yellow Sea	[39]
	Naseseazine A (19)	Antiplasmodial	Streptomyces sp. USC-636	Marine sediment	[40]
	Naseseazine B (20)	Antiplasmodial	Streptomyces sp. USC-636	Marine sediment	[40]
	Naseseazine C (21)	Antiplasmodial	Streptomyces sp. USC-636	Marine sediment	[40]
	(6R,3Z)-3-Benzylidene-6-isobutyl-1-methyl piperazine-2,5-dione (22)	-	Streptomyces sp. strain SCSIO 04496	South China Sea	[41]
	Demethylmaremycins (23–28)	-	Streptomyces sp. B9173	Pacific coast	[42]
	3-(3-Hydroxy-4-methoxybenzyl)-6-isobutyl-2,5-diketopiperazine (29)	Cytotoxicity	Streptomyces sp. MNU FJ-36	Katsuwonus sp.	[43]
	3-(1,3-Benzodioxol-5-ylmethyl)-6-isobutyl-2,5-diketopiperazine (30)	Cytotoxicity	Streptomyces sp. MNU FJ-36	Katsuwonus sp.	[43]
	3-(1,3-Benzodioxol-5-ylmethyl)-6-isopropyl-2,5-diketopiperazine (31)	Cytotoxicity	Streptomyces sp. MNU FJ-36	Katsuwonus sp.	[43]
	maculosin-O-α-L-rhamnopyranoside (32)	Antimicrobial	Streptomyces sp. ZZ446	Coastal soil	[44,45]
	Actinozine A (33)	Antimicrobial Cytotoxicity	Streptomyces species Call-36	Callyspongia species	[46]
	Streptodiketopiperazine A (34)	Antimicrobial	Streptomyces sp. SY1965	Mariana Trench sediment	[47]
	Streptodiketopiperazine A (35)	Antimicrobial	Streptomyces sp. SY1965	Mariana Trench sediment	[47]
	Cyclo-(4-trans-6-dihydroxy-proline-D-leucine) (36)	-	M. variabilis C-03	Palythoa tuberculosa	[48]
	Nocarazepine A (37)	-	A. caerulea	Anthogorgia caerulea	[49]
	Photopiperazine A (38)	Cytotoxicity	Strain AJS-327	Sponge fragment	[50]
	Photopiperazine B (39)	Cytotoxicity	Strain AJS-327	Sponge fragment	[50]
	Photopiperazine C (40)	Cytotoxicity	Strain AJS-327	Sponge fragment	[50]
	Photopiperazine D (41)	Cytotoxicity	Strain AJS-327	Sponge fragment	[50]
	Cyclo-(D-8-acetoxyl-Pro-L-Leu) (42)	Pancreatic lipase enzyme inhibition	Treptomyces sp. SCSIO 41400	Mangrove derived-soil	[51]
Fungus	Graphiumin A (43)	-	Graphium sp. OPMF00224	Sediment	[52]
	Graphiumin B (44)	-	Graphium sp. OPMF00224	Sediment	[52]
	Graphiumin C (45)	Virulence factors inhibitor	Graphium sp. OPMF00224	Sediment	[52]
	Graphiumin D (46)	Virulence factors inhibitor	Graphium sp. OPMF00224	Sediment	[52]
	Graphiumin E (47)	Virulence factors inhibitor	Graphium sp. OPMF00224	Sediment	[52]
	Graphiumin F (48)	-	Graphium sp. OPMF00224	Sediment	[52]
	Graphiumin G (49)	Virulence factors inhibitor	Graphium sp. OPMF00224	Sediment	[52]
	Graphiumin H (50)	Virulence factors inhibitor	Graphium sp. OPMF00224	Sediment	[52]
	Graphiumin I (51)	Virulence factors inhibitor	Graphium sp. OPMF00224	Sediment	[53]
	Graphiumin J (52)	Virulence factors inhibitor	Graphium sp. OPMF00224	Sediment	[53]
	Cladosporin A (53)	Cytotoxicity	Cladosporium sp.	Sediment	[54]
	Cladosporin B (54)	Cytotoxicity	Cladosporium sp.	Sediment	[54]
	12,13-Dihydroxy-fumitremorgin C (55)	-	A. alternate HK-25	Sediment	[55]
	Gliotoxin (56)	-	A. alternate HK-25	Sediment	[55]
	Demethoxyfumitremorgin C (57)	-	A. alternate HK-25	Sediment	[55]
	Bisdethiobis(methylthio)gliotoxin (58)	-	A. alternate HK-25	Sediment	[55]
	Fumitremorgin C (59)	-	A. alternate HK-25	Sediment	[55]
	Haenamindole (60)	Antiviral	Penicillium sp. KCB12F005	Sediment	[56]
	Dichotocejpin A (61)	Cytotoxicity α-glucosidase inhibitor	D. cejpii FS110	Sediment	[57,58]
	Dichotocejpin B (62)	-	D. cejpii FS110	Sediment	[57,58]
	Dichotocejpin C (63)	-	D. cejpii FS110	Sediment	[57,58]
	Cristazine (64)	Antioxidant Cytotoxicity	C. cristatum	Sediment	[59,60]
	Eutypellazine A (65)	Antiviral	Eutypella sp. MCCC 3A00281	Sediment	[61]
	Eutypellazine B (66)	Antiviral	Eutypella sp. MCCC 3A00281	Sediment	[61]
	Eutypellazine C (67)	Antiviral	Eutypella sp. MCCC 3A00281	Sediment	[61]
	Eutypellazine D (68)	Antiviral	Eutypella sp. MCCC 3A00281	Sediment	[61]
	Eutypellazine E (69)	Antiviral	Eutypella sp. MCCC 3A00281	Sediment	[61]
	Eutypellazine F (70)	Antiviral	Eutypella sp. MCCC 3A00281	Sediment	[61]
	Eutypellazine G (71)	Antiviral	Eutypella sp. MCCC 3A00281	Sediment	[61]
	Eutypellazine H (72)	Antiviral	Eutypella sp. MCCC 3A00281	Sediment	[61]
	Eutypellazine I (73)	Antiviral	Eutypella sp. MCCC 3A00281	Sediment	[61]
	Eutypellazine J (74)	Antiviral	Eutypella sp. MCCC 3A00281	Sediment	[61]
	Eutypellazine K (75)	Antiviral	Eutypella sp. MCCC 3A00281	Sediment	[61]
	Eutypellazine L (76)	Antiviral	Eutypella sp. MCCC 3A00281	Sediment	[61]
	Eutypellazine M (77)	-	Eutypella sp. MCCC 3A00281	Sediment	[61]
	Eutypellazine N (78)	-	Eutypella sp. MCCC 3A00281	Sediment	[62]
	Eutypellazine O (79)	-	Eutypella sp. MCCC 3A00281	Sediment	[62]
	Eutypellazine P (80)	-	Eutypella sp. MCCC 3A00281	Sediment	[62]
	Eutypellazine Q (81)	-	Eutypella sp. MCCC 3A00281	Sediment	[62]
	Eutypellazine R (82)	-	Eutypella sp. MCCC 3A00281	Sediment	[62]
	Eutypellazine S (83)	-	Eutypella sp. MCCC 3A00281	Sediment	[62]
	Fusaperazine F (84)	Cytotoxicity	P. crustosum HDN153086	Sediment	[63]
	Roquefortine J (85)	Cytotoxicity	P. granulatum MCCC 3A00475	Sediment	[64,65]
	(+)-7,8-Epoxy-brevianamide Q (86)	-	A. versicolor MF180151	Sediment	[66]
	(−)-7,8-Epoxy-brevianamide Q (87)	-	A. versicolor MF180151	Sediment	[66]
	(+)-8-Hydroxy-brevianamide R (88)	-	A. versicolor MF180151	Sediment	[66]
	(−)-8-Hydroxy-brevianamide R (89)	-	A. versicolor MF180151	Sediment	[66]
	(+)-8-Epihydroxy-brevianamide R (90)	-	A. versicolor MF180151	Sediment	[66]
	(−)-8-Epihydroxy-brevianamide R (91)	-	A. versicolor MF180151	Sediment	[66]
	Raistrickindole A (92)	Antiviral	P. raistrickii IMB17-034	Sediment	[67]
	5′-Hydroxy-6′-ene-epicoccin G (93)	-	E. nigrum SD-388	Sediment	[68]
	7-Methoxy-7′-hydroxyepicoccin G (94)	-	E. nigrum SD-388	Sediment	[68]
	8′-Acetoxyepicoccin D (95)	-	E. nigrum SD-388	Sediment	[68]
	7′-Demethoxyrostratin C (96)	Cytotoxicity	E. nigrum SD-388	Sediment	[68]
	(+)-5-hydroxydiphenylalazine A (97)	-	E. nigrum SD-388	Sediment	[68]
	(−)-5-hydroxydiphenylalazine A (98)	-	E. nigrum SD-388	Sediment	[68]
	(+) Eurotinoid A (99)	Antioxidant	Eurotium sp. SCSIO F452	Sediment	[69]
	(−) Eurotinoid A (100)	Antioxidant	Eurotium sp. SCSIO F452	Sediment	[69]
	(+) Eurotinoid B (101)	Antioxidant	Eurotium sp. SCSIO F452	Sediment	[69]
	(−) Eurotinoid B (102)	Antioxidant	Eurotium sp. SCSIO F452	Sediment	[69]
	(+) Eurotinoid C (103)	Antioxidant	Eurotium sp. SCSIO F452	Sediment	[69]
	(−) Eurotinoid C (104)	Antioxidant	Eurotium sp. SCSIO F452	Sediment	[69]
	Versicamide A (105)	-	A. versicolor HDN08-60	Sediment	[70]
	Versicamide B (106)	-	A. versicolor HDN08-60	Sediment	[70]
	Versicamide C (107)	-	A. versicolor HDN08-60	Sediment	[70]
	Versicamide D (108)	-	A. versicolor HDN08-60	Sediment	[70]
	Versicamide E (109)	-	A. versicolor HDN08-60	Sediment	[70]
	Versicamide F (110)	-	A. versicolor HDN08-60	Sediment	[70]
	Versicamide G (111)	-	A. versicolor HDN08-60	Sediment	[70]
	Versicamide H (112)	Cytotoxicity	A. versicolor HDN08-60	Sediment	[70]
	Rubrumline A (113)	-	E. rubrum	Sediment	[71]
	Rubrumline B (114)	-	E. rubrum	Sediment	[71]
	Rubrumline C (115)	-	E. rubrum	Sediment	[71]
	Rubrumline D (116)	Antiviral	E. rubrum	Sediment	[71]
	Rubrumline E (117)	-	E. rubrum	Sediment	[71]
	Rubrumline F (118)	-	E. rubrum	Sediment	[71]
	Rubrumline G (119)	-	E. rubrum	Sediment	[71]
	Rubrumline H (120)	-	E. rubrum	Sediment	[71]
	Rubrumline I (121)	-	E. rubrum	Sediment	[71]
	Rubrumline J (122)	-	E. rubrum	Sediment	[71]
	Rubrumline K (123)	-	E. rubrum	Sediment	[71]
	Rubrumline L (124)	-	E. rubrum	Sediment	[71]
	Rubrumline M (125)	-	E. rubrum	Sediment	[71]
	Rubrumline N (126)	-	E. rubrum	Sediment	[71]
	Rubrumline O (127)	-	E. rubrum	Sediment	[71]
	12β-Hydroxy-13α-ethoxyverruculogen TR-2 (128)	-	Penicillium sp. DT-F29	Sediment	[72]
	12β-Hydroxy-13α-butoxyethoxyverruculogen TR-2 (129)	-	Penicillium sp. DT-F29	Sediment	[72]
	Hydrocycloprostatin A (130)	-	Penicillium sp. DT-F29	Sediment	[72]
	Hydrocycloprostatin B (131)	-	Penicillium sp. DT-F29	Sediment	[72]
	25-Hydroxyfumitremorgin B (132)	-	Penicillium sp. DT-F29	Sediment	[72]
	12β-Hydroxy-13α-butoxyethoxyfumitremorgin B (133)	-	Penicillium sp. DT-F29	Sediment	[72]
	12β-Hydroxy-13α-methoxyverruculogen (134)	BRD4 protein inhibition	Penicillium sp. DT-F29	Sediment	[72]
	26α-Hydroxyfumitremorgin A (135)	-	Penicillium sp. DT-F29	Sediment	[72]
	25-Hydroxyfumitremorgin A (136)	-	Penicillium sp. DT-F29	Sediment	[72]
	Diprostatin A (137)	BRD4 protein inhibition	Penicillium sp. DT-F29	Sediment	[72]
	(+) variecolortin A (138)	Antioxidant	Eurotium sp. SCSIO F452	Sediment	[73]
	(−) variecolortin A (139)	-	Eurotium sp. SCSIO F452	Sediment	[73]
	(+) variecolortin B (140)	Cytotoxicity	Eurotium sp. SCSIO F452	Sediment	[73]
	(−) variecolortin B (141)	-	Eurotium sp. SCSIO F452	Sediment	[73]
	(+) variecolortin C (142)	Cytotoxicity	Eurotium sp. SCSIO F452	Sediment	[73]
	(−) variecolortin C (143)	-	Eurotium sp. SCSIO F452	Sediment	[73]
	Eurotiumin A (144)	Antioxidant	Eurotium sp. SCSIO F452	Sediment	[74]
	Eurotiumin B (145)	Antioxidant	Eurotium sp. SCSIO F452	Sediment	[74]
	Eurotiumin C (146)	Antioxidant	Eurotium sp. SCSIO F452	Sediment	[74]
	6-Acetylmonodethiogliotoxin (147)	NF-κB inhibitor	D. cejpii	Callyspongia cf. C. flammea	[75]
	6-Acetylbisdethiobis(methylthio)gliotoxin (148)	NF-κB inhibitor	D. cejpii	Callyspongia cf. C. flammea	[75]
	5a,6-Anhydrobisdethiobis(methyl-thio)gliotoxin (149)	NF-κB inhibitor	D. cejpii	Callyspongia cf. C. flammea	[75]
	Peniciadametizine A (150)	Antimicrobial	P. adametzioides AS-53	Unidentified sponge	[76]
	peniciadametizine B (151)	Antimicrobial	P. adametzioides AS-53	Unidentified sponge	[76]
	Cyclo-(2-hydroxy-Pro-Gly) (152)	-	Simplicillium sp. YZ-11	H. perleve	[77]
	Fellutanine A (153)	-	N. glabra KUFA 0702	Mycale sp.	[78]
	Asperflocin (154)	Cytotoxicity	A. versicolor 16F-11	P. fusca	[79]
	Unnamed diketopiperazine dimer (155)	Anti-inflammatory	A. violaceofuscus	Reniochalina sp.	[80]
	Waspergillamide B (156)	-	A. ochraceus	A. oroides	[81]
	Penicillivinacine (157)	Cytotoxicity	P. vinaceum	H. erectus	[82]
	Adametizine A (158)	Antimicrobial Brine shrimp lethality	P. adametzioides AS-53	unidentified sponge	[83]
	Adametizine B (159)	Antimicrobial Brine shrimp lethality	P. adametzioides AS-53	unidentified sponge	[83]
	SF5280-415 (160)	Enzyme inhibition	Aspergillus sp. SF-5280	unidentified sponge	[84]
	Penicimutide (161)	Cytotoxicity	P. purpurogenum G59	Bohai Bay	[85]
	Penicimutanin C (162)	Cytotoxicity	P. purpurogenum G59	Bohai Bay	[86]
	Waikikiamide A (163)	Cytotoxicity	Aspergillus sp. FM242	Waikiki beach	[87]
	Waikikiamide B (164)	-	Aspergillus sp. FM242	Waikiki beach	[87]
	Waikikiamide C (165)	Cytotoxicity	Aspergillus sp. FM242	Waikiki beach	[87]
	5S-Hydroxynorvaline-S-Ile (166)	-	Penicillium sp. GD6	B. gymnorrhiza	[88]
	3S-Hydroxylcyclo(S-Pro-S-Phe) (167)	-	Penicillium sp. GD6	B. gymnorrhiza	[88]
	Cyclo(S-Phe-S-Gln) (168)	-	Penicillium sp. GD6	B. gymnorrhiza	[88]
	(−)-Asperginulin A (169)	-	Aspergillus sp. SK-28	K. candel	[89]
	(+)-Asperginulin A (170)	Antifouling	Aspergillus sp. SK-28	K. candel	[89]
	Saroclazine A (171)	Cytotoxicity	S. kiliense HDN11-84	T. populnea	[90]
	Saroclazine B (172)	-	S. kiliense HDN11-84	T. populnea	[90]
	Saroclazine C (173)	-	S. kiliense HDN11-84	T. populnea	[90]
	Brocazine A (174)	Cytotoxicity	P. brocae MA-231	A. marina	[91]
	Brocazine B (175)	Cytotoxicity	P. brocae MA-231	A. marina	[91]
	Brocazine C (176)	Cytotoxicity	P. brocae MA-231	A. marina	[91]
	Brocazine D (177)	Cytotoxicity	P. brocae MA-231	A. marina	[91]
	Brocazine E (178)	-	P. brocae MA-231	A. marina	[91]
	Brocazine F (179)	-	P. brocae MA-231	A. marina	[91]
	Penicibrocazine A (180)	-	P. brocae MA-231	A. marina	[92]
	Penicibrocazine B (181)	-	P. brocae MA-231	A. marina	[92]
	Penicibrocazine C (182)	Antimicrobial	P. brocae MA-231	A. marina	[92]
	Penicibrocazine D (183)	-	P. brocae MA-231	A. marina	[92]
	Penicibrocazine E (184)	Antimicrobial	P. brocae MA-231	A. marina	[92]
	Spirobrocazine A (185)	Antimicrobial	P. brocae MA-231	A. marina	[93]
	Spirobrocazine B (186)	-	P. brocae MA-231	A. marina	[93]
	Spirobrocazine C (187)	Antimicrobial	P. brocae MA-231	A. marina	[93]
	Brocazine G (188)	Antimicrobial Cytotoxicity	P. brocae MA-231	A. marina	[93]
	Pseudellone A (189)	-	P. ellipsoidea F42−3	L. crassum	[94]
	Pseudellone B (190)	-	P. ellipsoidea F42−3	L. crassum	[94]
	Pseuboydone C (191)	Cytotoxicity	P. boydii F19-1	L. crassum	[95]
	Pseuboydone D (192)	-	P. boydii F19-1	L. crassum	[95]
	Dichocerazine A (193)	-	D. cejpii F31-1	L. crassum	[96]
	Dichocerazine B (194)	-	D. cejpii F31-1	L. crassum	[96]
	11-Methylneoechinulin E (195)	Cytotoxicity	Aspergillus sp. EGF 15-0-3	South China Sea	[97]
	Variecolorin M (196)	Cytotoxicity	Aspergillus sp. EGF 15-0-3	South China Sea	[97]
	(+)-Variecolorin G (197)	Cytotoxicity	Aspergillus sp. EGF 15-0-3	South China Sea	[97]
	(+)-Neoechinulin A (198)	Cytotoxicity	Aspergillus sp. EGF 15-0-3	South China Sea	[97]
	16α-Hydroxy-17β-methoxy-deoxydihydroisoaustamide (199)	-	P. dimorphosporum KMM 4689	South China Sea	[98]
	16β-Hydroxy-17α-methoxy-deoxydihydroisoaustamide (200)	-	P. dimorphosporum KMM 4689	South China Sea	[98]
	16β,17α-dihydroxy-deoxydihydroisoaustamide (201)	-	P. dimorphosporum KMM 4689	South China Sea	[98]
	16α-hydroxy-17α-methoxy-deoxydihydroisoaustamide (202)	Enhance cell viability	P. dimorphosporum KMM 4689	South China Sea	[98]
	16α,17α-dihydroxy-deoxydihydroisoaustamide (203)	Enhance cell viability	P. dimorphosporum KMM 4689	South China Sea	[98]
	16,17-dihydroxy-deoxydihydroisoaustamide (204)	Enhance cell viability	P. dimorphosporum KMM 4689	South China Sea	[98]
	3β-Hydroxy-deoxyisoaustamide (205)	-	P. dimorphosporum KMM 4689	South China Sea	[98]
	Pseudellone D (206)	-	P. ellipsoidea F42-3	L. crissum	[99]
	Dehydroxymethylbis(dethio)bis(methylthio)gliotoxin (207)	-	T. virens Y13-3	G. vermiculoph-ylla	[100]
	(3S,6R)-6-(Para-hydroxybenzyl)-1,4-dimethyl-3,6-bis(methylthio)pip-erazine-2,5-dione (208)	-	T. virens Y13-3	G. vermiculoph-ylla	[100]
	Methylcordysinin A (209)	-	T. asperellum cf44-2	Sargassum sp.	[101]
	Citriperazine A (210)	-	Penicillium sp. KMM 4672	Padina sp.	[102]
	Citriperazine B (211)	-	Penicillium sp. KMM 4672	Padina sp.	[102]
	Citriperazine C (212)	-	Penicillium sp. KMM 4672	Padina sp.	[102]
	Citriperazine D (213)	-	Penicillium sp. KMM 4672	Padina sp.	[102]
	(+) Acrozines A (214)	Antiacetylcholinesterase	A. luteoalbus TK-43	C. fragile	[103]
	(−) Acrozines A (215)	Antiacetylcholinesterase	A. luteoalbus TK-43	C. fragile	[103]
	(+) Acrozines B (216)	Antimicrobial	A. luteoalbus TK-43	C. fragile	[103]
	(−) Acrozines B (217)	-	A. luteoalbus TK-43	C. fragile	[103]
	(+) Acrozines C (218)	-	A. luteoalbus TK-43	C. fragile	[103]
	(−) Acrozines C (219)	-	A. luteoalbus TK-43	C. fragile	[103]
	Cyclo(L-5-MeO-Pro-L-5-MeO-Pro) (220)	Antimicrobial	T. asperellum A-YMD-9-2	G. verrucose	[104]
	Pretrichodermamide D (221)	-	Penicillium sp. KMM 4672	Padina sp.	[105]
	Pretrichodermamide E (222)	-	Penicillium sp. KMM 4672	Padina sp.	[105]
	Pretrichodermamide F (223)	-	Penicillium sp. KMM 4672	Padina sp.	[105]
	N-(4′-hydroxyprenyl)-cyclo(alanyltryptophyl) (224)	-	E. cristatum EN-220	S. thunbergia	[106]
	Isovariecolorin I (225)	Brine shrimp lethal	E. cristatum EN-220	S. thunbergia	[106]
	30-Hydroxyechinulin (226)	-	E. cristatum EN-220	S. thunbergia	[106]
	29-Hydroxyechinulin (227)	-	E. cristatum EN-220	S. thunbergia	[106]
	(+)-Brevianamide X (228)	-	A. versicolor OUCMDZ-2738	E. prolifera	[107]
	(−)-Brevianamide X (229)	-	A. versicolor OUCMDZ-2738	E. prolifera	[107]
	Isoechinulin D (230)	-	E. rubrum MPUC136	/ b	[108]
	Spirotryprostatin G (231)	Cytotoxicity	P. brasilianum HBU-136	Bohai Sea	[109]
	Cyclotryprostatin F (232)	Cytotoxicity	P. brasilianum HBU-136	Bohai Sea	[109]
	Cyclotryprostatin G (233)	Cytotoxicity	P. brasilianum HBU-136	Bohai Sea	[109]
	Penilline C (234)	-	P. chrysogenum SCSIO 07007	Western Atlantic	[110]
	Emestrin L (235)	-	A. terreus RA2905	A. pulmonica	[111]
	Emestrin M (236)	Antimicrobial	A. terreus RA2905	A. pulmonica	[111]
	Aspamide A (237)	-	A. versicolor DY180635	C. haematocheir	[112]
	Aspamide B (238)	-	A. versicolor DY180635	C. haematocheir	[112]
	Aspamide C (239)	-	A. versicolor DY180635	C. haematocheir	[112]
	Aspamide D (240)	-	A. versicolor DY180635	C. haematocheir	[112]
	Penicillatide B (241)	Cytotoxicity Antimicrobial	Penicillium sp.	Didemnum sp.	[113]

^a The bioactivity was not mentioned; ^b the habitat was not mentioned.

Table 2

The bioactivities, strains and habitats of diketopiperazine derivatives during 2011–2021.

Sources	Compounds	Bioactivities	Species	Habitats	Refs
Actinomycetes	Isomethoxyneihumicin (242 and 243)	Cytotoxicity	N. alba KM6-1	Marine sediment	[114]
	Nocazine F (244)	Cytotoxicity	Nocardiopsis sp. YIM M13066	Deep-sea sediment	[115]
	Nocazine G (245)	Cytotoxicity Antimicrobial	Nocardiopsis sp. YIM M13066	Deep-sea sediment	[115]
	Streptopyrazinone A (246)	Antimicrobial	Streptomyces sp. ZZ446	Coastal soil	[44]
	Streptopyrazinone B (247)	Antimicrobial	Streptomyces sp. ZZ446	Coastal soil	[44]
	Streptopyrazinone C (248)	Antimicrobial	Streptomyces sp. ZZ446	Coastal soil	[44]
	Streptopyrazinone D (249)	Antimicrobial	Streptomyces sp. ZZ446	Coastal soil	[44]
	Streptopyrazinone (250)	Cytotoxicity	Streptomyces sp. B223	Marine sediment	[116]
	Nocazine A (251)	-	N. dassonvillei HR10-5	Estuary of Yellow River	[117]
	Nocazine B (252)	-	N. dassonvillei HR10-5	Estuary of Yellow River	[117]
Fungus	Varioloid A (253)	Antimicrobial	P. variotii EN-291	G. turuturu	[118]
	Varioloid B (254)	Antimicrobial	P. variotii EN-291	G. turuturu	[118]
	Oxepinamide H (255)	Transcriptional activation	A. puniceus SCSIO z021	Deep-sea sediment	[119]
	Oxepinamide I (256)	Transcriptional activation	A. puniceus SCSIO z021	Deep-sea sediment	[119]
	Oxepinamide J (257)	Transcriptional activation	A. puniceus SCSIO z021	Deep-sea sediment	[119]
	Oxepinamide K (258)	-	A. puniceus SCSIO z021	Deep-sea sediment	[119]
	Puniceloid A (259)	Transcriptional activation	A. puniceus SCSIO z021	Deep-sea sediment	[119]
	Puniceloid B (260)	Transcriptional activation	A. puniceus SCSIO z021	Deep-sea sediment	[119]
	Puniceloid C (261)	Transcriptional activation	A. puniceus SCSIO z021	Deep-sea sediment	[119]
	Puniceloid D (262)	Transcriptional activation Enzyme inhibition	A. puniceus SCSIO z021	Deep-sea sediment	[119]
	Protuboxepin C (263)	Cytotoxicity	Aspergillus sp. SCSIO XWS02F40	Callyspongia sp.	[120,121]
	Protuboxepin D (264)	Cytotoxicity	Aspergillus sp. SCSIO XWS02F40	Callyspongia sp.	[120,121]
	Pyranamide A (265)	-	A. versicolor SCSIO 41016	Sponge	[122]
	Pyranamide A (266)	-	A. versicolor SCSIO 41016	Sponge	[122]
	Pyranamide A (267)	-	A. versicolor SCSIO 41016	Sponge	[122]
	Pyranamide A (268)	-	A. versicolor SCSIO 41016	Sponge	[122]
	Secopyranamide C (269)	-	A. versicolor SCSIO 41016	Sponge	[122]
	Protuboxepin F (270)	Cytotoxicity	A. versicolor SCSIO 41016	Sponge	[122]
	Protuboxepin G (271)	-	A. versicolor SCSIO 41016	Sponge	[122]
	Protuboxepin H (272)	-	A. versicolor SCSIO 41016	Sponge	[122]
	Protuboxepin I (273)	-	A. versicolor SCSIO 41016	Sponge	[122]
	Protuboxepin J (274)	-	A. versicolor SCSIO 41016	Sponge	[122]
	Chrysopiperazine A (275)	-	P. chrysogenum	D. gemmacea	[123]
	Chrysopiperazine B (276)	-	P. chrysogenum	D. gemmacea	[123]
	Chrysopiperazine C (277)	-	P. chrysogenum	D. gemmacea	[123]
	Quinadoline D (278)	-	Penicillium sp. L129	L. sinense	[124]
	Aspamide F (279)	-	A. versicolor DY180635	C. haematocheir	[110]
	Aspamide G (280)	-	A. versicolor DY180635	C. haematocheir	[110]
	Polonimide A (281)	Enzyme inhibition	P. polonicum	Bohai Sea	[125]
	Polonimide B (282)	Enzyme inhibition	P. polonicum	Bohai Sea	[125]
	Polonimide C (283)	Enzyme inhibition	P. polonicum	Bohai Sea	[125]
	Protuboxepin K (284)	Enzyme inhibition	Aspergillus sp. BFM-0085	Marine sediment	[126]
	Varioxepine B (285)	Cytotoxicity	A. terreus	S. subviride	[127]
	3-Hydroxyprotuboxepin K (286)	Enzyme inhibition	A. creber EN-602	R. confervoides	[128]
	3,15-Hehydroprotuboxepin K (287)	Antimicrobial	A. creber EN-602	R. confervoides	[128]
	Versiamide A (288)	Antimicrobial	A. creber EN-602	R. confervoides	[128]
	Protuboxepin A (289)	Cytotoxicity	Aspergillus sp. SF-5044	Sediment	[129,130]
	Protuboxepin B (290)	-	Aspergillus sp. SF-5044	Sediment	[129,130]
	Carnequinazoline A (291)	-	A. carneus KMM 4638	L. sachalinensis	[131]
	Carnequinazoline B (292)	-	A. carneus KMM 4638	L. sachalinensis	[131]
	Carnequinazoline C (293)	-	A. carneus KMM 4638	L. sachalinensis	[131]
	Fumiquinazoline K (294)	-	A. fumigatus KMM 4631	Sinularia sp.	[132]
	3-[6-(2-Methylpropyl)-2-oxo-1H-pyrazin-3-yl]propanamide (295)	-	A. versicolor OUCMDZ-2738	E. prolifera	[107]

^a The bioactivity was not mentioned.