Abstract/Details

Accounting for Patient Variability in Designing Biomaterials and Therapeutic Regenerative Strategies

DeBari, Megan K.  Carnegie Mellon University, ProQuest Dissertations Publishing, 2021. 28720196.

Abstract (summary)

Critically sized defects in subcutaneous adipose tissue result in extensive disfigurement and dysfunction and remain a reconstructive challenge for surgeons. Defects can occur from traumatic injury, ablative procedures, and congenital malformations and are currently treated with autologous fat grafts [1]. Researchers have shown that autologous fat grafting is less effective for patients with low stromal vascular fractions (SVF) because these cells are responsible for creating angiogenic and anti-inflammatory environments [2]. Obesity and adipose tissue fibrosis are negatively correlated with the SVF and stem cell concentrations [3]. Not only are stem cells less abundant in fibrotic adipose tissue, but they are also predisposed to become profibrotic cells instead of adipocytes [4]. This work evaluates the biological and mechanical properties of adipose tissue to better understand how patient demographics affect degree of fibrosis and regenerative capacity. The data gathered from this study will be used to inform soft tissue regenerative approaches and in vitro modeling of adipose tissue fibrosis. This work also evaluates using decellularized lung matrices as viable in vitro 3D vascularized adipose tissue models. Along with comorbidities, a patient’s capacity for tissue regeneration varies based on age, nutritional status, lifestyle, gender, race, etc. Because regeneration rate cannot be predicted prior to biomaterial implantation, there is a need for responsive biomaterials with adaptive, personalized degradation rates to improve regenerative outcomes. This research focused on silk as an ultrasound-responsive biomaterial, where degradation rates can be altered non-invasively to improve regenerative outcomes. While this research seeks to improve soft tissue engineering for patients afflicted with metabolic diseases by accounting for variations in regenerative potential, it will also enhance our knowledge on the biological and mechanical properties of fibrotic adipose tissue.

Indexing (details)


Subject
Biomedical engineering;
Materials science;
Morphology;
Cellular biology;
Histology
Classification
0794: Materials science
0541: Biomedical engineering
0287: Morphology
0379: Cellular biology
0414: Histology
Identifier / keyword
Patient variability; Biomaterials; Therapeutic regenerative strategies
Title
Accounting for Patient Variability in Designing Biomaterials and Therapeutic Regenerative Strategies
Author
DeBari, Megan K.  VIAFID ORCID Logo 
Number of pages
268
Publication year
2021
Degree date
2021
School code
0041
Source
DAI-B 83/2(E), Dissertation Abstracts International
Place of publication
Ann Arbor
Country of publication
United States
ISBN
9798538137626
Advisor
Abbott, Rosalyn
Committee member
Feinberg, Adam; Cohen-Karni, Tzahi; Campbell, Phil
University/institution
Carnegie Mellon University
Department
Materials Science and Engineering
University location
United States -- Pennsylvania
Degree
Ph.D.
Source type
Dissertation or Thesis
Language
English
Document type
Dissertation/Thesis
Dissertation/thesis number
28720196
ProQuest document ID
2572570650
Copyright
Database copyright ProQuest LLC; ProQuest does not claim copyright in the individual underlying works.
Document URL
https://www.proquest.com/docview/2572570650