Abstract

Biphenyl 2,3-dioxygenase (BphA) is an enzyme that participates in initiation step of the pathway of biphenyl degradation in bacteria. Biphenyl is an aromatic hydrocarbon including two benzene rings that causes a serious environmental pollution. This report presents the preliminary results on the cloning and expression of BphA gene of B. cepacia strains DF2 and DF4 which were isolated from dioxin contaminated soil in Vietnam. The coding DNA sequences of BphA genes are 897 bp long and 99% identical. Recombinant BphA enzyme (33 kDa) has strongly expressed in E. coli M15 after induction of 0.5 mM IPTG for 4 h at 37ºC.

Details

Title
HETEROLOGOUS EXPRESSION OF A GENE ENCODING BIPHENYL 2,3-DIOXYGENASE FROM BURKHOLDERIA CEPACIA IN ESCHERICHIA COLI
Author
Nguyen, Hoang Loc; Duong Duc Hoang Sinh; Nguyen, Hoang Tue; Nguyen, Duc Huy; Tran Vu Ngoc Thi; Le Thi Ha Thanh
Pages
490-492
Section
Biotechnology
Publication year
2020
Publication date
Dec 2020/Jan 2021
Publisher
Faculty of Biotechnology and Food Sciences
e-ISSN
13385178
Source type
Scholarly Journal
Language of publication
English
ProQuest document ID
2576924990
Copyright
© 2020. This work is published under https://creativecommons.org/licenses/by-nd/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.