Content area
Full Text
Abstract
Snakehead fish (Channa striata) is a freshwater fish that has a high protein content which is needed for the formation of new cell tissue such as wound healing due to surgery. Extract contains amino acids and fatty acids that are important in the synthesis of collagen fibers, especially glycine, during the wound healing process. Snakehead fish albumin is very good for people with hypoalbumin and postoperative wound healing and burns. Freeze dry is done so that there is no remaining moisture content so that the sample can be more stable in storage. The purpose of this test was to determine the albumin content and water phase protein content of snakehead fish (Channa striata) extract before and after freeze dry. The aqueous phase sample of snakehead fish extract was tested for albumin content by heating method and analyzed for protein content using visible spectrophotometry based on the absorption of purple light from the protein reacting with Biuret reagent. The results of the analysis of albumin content in the aqueous phase of snakehead fish extract were analyzed qualitatively using the heating method. The results of the analysis showed that the albumin content in the water phase of snakehead fish extract and the total protein content of the snakehead fish water phase extract before freeze dry was 21775 ppm while after freeze dry was smaller, namely 16876 ppm where the difference in levels decreased The total protein in the aqueous phase of snakehead fishextract before and after freeze dry was 22.489%.
Keywords: albumin, biuret method, freeze dry, protein content, water phase of snakehead fishextract.
Introduction
Snakehead fish is one of the endemic swamp fish species whose presence is decreasing.[1] Snakehead fish (Channa striata) is a swamp fish that has not been widely cultivated. This fish is a type of freshwater carnivorous fish that inhabits the Southeast Asian region. Snakehead fish albumin is very good for people with hypoalbumin (low albumin), and postoperative wound healing and burns. Albumin is used in controlling the nutritional status of acute and chronic sick patients.[1] The nutritional content of snakehead fish consists of high protein content, especially albumin and essential amino acids, fats, especially essential fatty acids, and minerals, especially zinc/zinc (Zn) which are needed for cell development and the formation...