Abstract

l-Lactate, traditionally considered a metabolic waste product, is increasingly recognized as an important intercellular energy currency in mammals. To enable investigations of the emerging roles of intercellular shuttling of l-lactate, we now report an intensiometric green fluorescent genetically encoded biosensor for extracellular l-lactate. This biosensor, designated eLACCO1.1, enables cellular resolution imaging of extracellular l-lactate in cultured mammalian cells and brain tissue.

l-lactate is an important intercellular energy currency. Here the authors report a genetically encoded biosensor eLACCO1.1 to monitor extracellular l-lactate; they use eLACCO1.1 to image extracellular l-lactate in cultured mammalian cells and brain tissue.

Details

Title
A genetically encoded fluorescent biosensor for extracellular l-lactate
Author
Nasu Yusuke 1 ; Murphy-Royal, Ciaran 2   VIAFID ORCID Logo  ; Wen Yurong 3 ; Haidey, Jordan N 4 ; Molina, Rosana S 5   VIAFID ORCID Logo  ; Aggarwal Abhi 6 ; Zhang Shuce 7   VIAFID ORCID Logo  ; Kamijo Yuki 1 ; Paquet Marie-Eve 8   VIAFID ORCID Logo  ; Podgorski Kaspar 6   VIAFID ORCID Logo  ; Drobizhev Mikhail 5   VIAFID ORCID Logo  ; Bains, Jaideep S 4 ; Joanne, Lemieux M 9   VIAFID ORCID Logo  ; Gordon, Grant R 4   VIAFID ORCID Logo  ; Campbell, Robert E 10   VIAFID ORCID Logo 

1  The University of Tokyo, Bunkyo-ku, Department of Chemistry, School of Science, Tokyo, Japan (GRID:grid.26999.3d) (ISNI:0000 0001 2151 536X) 
2  Hotchkiss Brain Institute, Cumming School of Medicine, Department of Physiology and Pharmacology, University of Calgary, Calgary, Canada (GRID:grid.22072.35) (ISNI:0000 0004 1936 7697); Centre Hospitalier de l’Université de Montréal, Department of Neuroscience, Faculty of Medicine, University of Montreal, Montreal, Canada (GRID:grid.410559.c) (ISNI:0000 0001 0743 2111) 
3  University of Alberta, Department of Biochemistry, Edmonton, Canada (GRID:grid.17089.37); The First Affiliated Hospital, Xi’an Jiaotong University, Department of Talent Highland, Xi’an, China (GRID:grid.452438.c) (ISNI:0000 0004 1760 8119) 
4  Hotchkiss Brain Institute, Cumming School of Medicine, Department of Physiology and Pharmacology, University of Calgary, Calgary, Canada (GRID:grid.22072.35) (ISNI:0000 0004 1936 7697) 
5  Montana State University, Department of Microbiology and Cell Biology, Bozeman, USA (GRID:grid.41891.35) (ISNI:0000 0001 2156 6108) 
6  Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, USA (GRID:grid.443970.d) 
7  University of Alberta, Department of Chemistry, Edmonton, Canada (GRID:grid.17089.37) 
8  CERVO Brain Research Center, Laval University, Québec, Canada (GRID:grid.23856.3a) (ISNI:0000 0004 1936 8390); Microbiology and Bioinformatics, Laval University, Department of Biochemistry, Québec, Canada (GRID:grid.23856.3a) (ISNI:0000 0004 1936 8390) 
9  University of Alberta, Department of Biochemistry, Edmonton, Canada (GRID:grid.17089.37) 
10  The University of Tokyo, Bunkyo-ku, Department of Chemistry, School of Science, Tokyo, Japan (GRID:grid.26999.3d) (ISNI:0000 0001 2151 536X); University of Alberta, Department of Chemistry, Edmonton, Canada (GRID:grid.17089.37) 
Publication year
2021
Publication date
2021
Publisher
Nature Publishing Group
e-ISSN
20411723
Source type
Scholarly Journal
Language of publication
English
DOI
https://doi.org/10.1038/s41467-021-27332-2
ProQuest document ID
2607083424
Copyright
© The Author(s) 2021. This work is published under http://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.