Morphological characteristics and accumulation of arsenic in Argyrochosma formosa (Liebm.) Windham developed in a highly contaminated site with arsenic in Matehuala, SLP, México

Abstract

We studied the ability of Argyrochosma formosa growing in an arsenic heavily contaminated site to accumulate this metalloid; morphological characteristics and translocation of arsenic were evaluated in the organs. Population census of wild specimens of A. formosa was done, and 14 samples of ferns and rhizosphere soil were collected randomly. We recorded morphological characteristics with scanning electronic microscopy (SEM); concentrations of As in organs of fern plants (root, rhizome, and fronds) were evaluated with inductively coupled plasma-optic emission spectrometry (ICP-OES). Two hundred ninety-four individuals at different stages of development were identified, indicating the establishment of fern on the site. Morphological characteristics of A. formosa in fern plant organs did not show structural effects, compared with herbarium plants. Arsenic distribution in fern plant tissues was 192.2–763.6 mg/kg, 188–1017 mg/kg, and 113–2008 mg/kg, in roots, rhizomes, and fronds, respectively. The calculated bioaccumulation factor in fronds ranged from 2 to 7 and the translocation factor from 0.6 to 2.1. Our data suggest that A. formosa is an arsenic-tolerant species and propose it for phytoremediation on contaminated sites with As concentrations similar to that of the studied location. Further studies should be performed to evaluate the mechanisms of accumulation of As in plant tissues.