Exposure to Diesel Exhaust and Plasma Cortisol Response: A Randomized Double-Blind Crossover Study

Abstract

Experimental work has demonstrated that glucocorticoid stress hormones are potential mediators of pulmonary and systemic pollutant effects of both particulate and gaseous pollutants (Thomson 2019), but direct evidence of TRAP-dependent stress axis activation is lacking. [...]although oxidative stress is a regulator of glucocorticoid signaling (Okamoto et al. 1999), involvement in pollutant-induced stress axis activation is unknown. In brief, study participants [ n=19 n = 19 ; mean age 28 (range 19–46), 14 with doctor-diagnosed asthma (7 females, 7 males), and 5 without (3 females, 2 males); all free from current use of inhaled corticosteroids or long-acting β2 agonists β 2agonists , from regular use of bronchodilators, and from vitamin A, C, or E supplementation] took N-acetylcysteine (NAC; 600mg 600 mg ) or placebo capsules three times daily for 6 d. On day 6, participants were exposed for 2 h to filtered air or diesel exhaust, producing three experimental conditions [filtered air with placebo (FAP), diesel exhaust with placebo (DEP), and diesel exhaust with NAC (DEN)], using a randomized, double-blind, crossover design that included a minimum 2-wk washout period between exposures. [...]although we assessed antioxidant genes, other genetic variation could also influence cortisol responses.

Introduction

Traffic-related air pollution (TRAP) is associated with a variety of adverse health effects. Although a central role has been proposed for oxidative stress, elucidating underlying mechanisms remains an area of active investigation. Experimental work has demonstrated that glucocorticoid stress hormones are potential mediators of pulmonary and systemic pollutant effects of both particulate and gaseous pollutants (Thomson 2019), but direct evidence of TRAP-dependent stress axis activation is lacking. Moreover, although oxidative stress is a regulator of glucocorticoid signaling (Okamoto et al. 1999), involvement in pollutant-induced stress axis activation is unknown. In population studies, nitrogen dioxide was associated with a flattened salivary cortisol profile in adolescents (Wing et al. 2018) and higher awakening cortisol and flattened profile in 45 to 85-y-old adults (Hajat et al. 2019), suggesting potential TRAP-related impacts. The present study evaluated whether short-term exposure to diesel exhaust increases plasma cortisol levels and considered effect modification by sex, asthma status, antioxidant gene variants, and antioxidant treatment.

Methods

The study was approved by the University of British Columbia, Vancouver Coastal Health Research Institute, and Health Canada ethics review boards, and registered at clinicaltrials.gov (trial NCT01699204). Each participant provided written informed consent. Study details, including exclusion criteria, diet and medications, questionnaires, assessment of airway hyperresponsiveness, and genotyping, are provided elsewhere (Carlsten et al. 2014). In brief, study participants [ n=19 n = 19 ; mean age 28 (range 19–46), 14 with doctor-diagnosed asthma (7 females, 7 males), and 5 without (3 females, 2 males); all free from current use of inhaled corticosteroids or long-acting β2 agonists β 2agonists , from regular use of bronchodilators, and from vitamin A, C, or E supplementation] took N-acetylcysteine (NAC; 600mg 600 mg ) or placebo capsules three times daily for 6 d. On day 6, participants were exposed for 2 h to filtered air or diesel exhaust, producing three experimental conditions [filtered air with placebo (FAP), diesel exhaust with placebo (DEP), and diesel exhaust with NAC (DEN)], using a randomized, double-blind, crossover design that included a minimum 2-wk washout period between exposures. The protocol is nearly identical to another in which analysis of questionnaires demonstrated that participants were effectively blinded to exposures (Carlsten et al. 2013). Diesel exhaust was generated using a 6.0-kW 6.0-kW diesel generator operated to simulate on-road emissions and diluted to maintain a nominal particulate concentration of 300 μg/m3 300μg / m 3 [fine particulate matter (PM) with aerodynamic diameter less than or equal to 2.5μm 2.5 μm ( PM2.5 PM 2.5 ); mass median aerodynamic diameter of 102.5±14 nm 102.5 ± 14nm )]. Blood was collected by venipuncture into EDTA tubes 18 h prior to exposure, immediately before exposure (0 h), and at 2, 6, and 30 h. Plasma was assessed in duplicate using Arbor Assays cortisol enzyme immunoassay kits (Cedarlane Laboratories Canada). Blood samples from each volunteer underwent polymerase chain reaction (PCR)-restriction fragment length polymorphism analysis to measure the variant status of two present/null alleles (GSTM1, GSTT1; coded as 0 or 1 for null), and three single-nucleotide polymorphisms [SNPs; (GSTP1 rs1695; NFκB1 NFκ B 1 rs28362491; NQO1 rs1800566; coded as 0, 1, or 2 for homozygous risk variant)]. Genetic risk scores were generated using unweighted summation of null and risk alleles. Data were divided into high (4–6) and low (0–3) risk scores based on the median value. A generalized estimating equation approach was used to analyze the data using geepack library (version 1.3-1) in the R software (R Development Core Team) environment (Højsgaard et al. 2005). Cortisol levels were logarithmically transformed and analyzed, assuming a normal distribution and an exchangeable correlation structure. Results were back-transformed, and the delta method was used to approximate the standard error for the estimates, with Holm-Sidak adjustment to control the familywise error rate.

Results and Discussion

Short-term exposure to diesel exhaust rapidly and transiently increased plasma cortisol levels ( Exposure×Time E x p o s u r e × T i m e interaction; p<0.0001 p < 0.0001 ; DEP vs. FAP at 2 h; p=0.04 p = 0.04 ; Figure 1). The relative increase in cortisol during exposure to diesel exhaust compared with filtered air was similar in males and females and was observed primarily in those diagnosed with asthma or with gene deletions or polymorphisms in antioxidant genes (Figure 2).

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The data show that exposure to diesel exhaust affects levels of an important stress hormone with wide-ranging systemic effects, substantiating a role for cortisol as a potential mediator of TRAP-dependent health effects and providing support under controlled conditions for epidemiological associations between TRAP and cortisol. The variation in cortisol response to diesel exhaust across participants was in line with known variation in response to acute stressors within the population according to factors including age, genetic variability, disease, and chronic stress (Rohleder et al. 2003). Although genetic variability in antioxidant genes was associated with the magnitude of cortisol response, treatment with the antioxidant NAC tended to reduce, but did not eliminate, the diesel exhaust-dependent increase. It is unclear whether this reflects insufficient antioxidant supplementation to block effects, lack of involvement of oxidative stress in the response, or inadequate power to detect effect modification. Although contrasting with the hyporesponsiveness to psychosocial stressors of individuals with asthma, the heightened cortisol response to diesel exhaust in participants with asthma is similar to the greater cortisol response to inhaled allergen challenge produced in people with asthma (Peebles et al. 2000). Notwithstanding the association of asthma diagnosis with increased cortisol response, degree of airway hyperresponsiveness was not significantly associated with differential cortisol response to diesel exhaust ( r=−0.083 r = − 0.083 ; p=0.77 p = 0.77 ). The variability in cortisol responses to diesel exhaust seen here in relation to individual characteristics may have relevance to individual risk of local and systemic health effects of air pollutants (Thomas et al. 2018; Thomson 2019).

Limitations

Although the crossover design is statistically powerful, the study may nevertheless be underpowered to detect effect modification. Participants were predominantly young and had asthma; it is unknown whether effects would manifest similarly in other populations. Despite the washout period between exposures, participants will have been exposed to TRAP elsewhere, which could condition responses. Finally, although we assessed antioxidant genes, other genetic variation could also influence cortisol responses.

Acknowledgments

This work was supported by Health Canada through the Clean Air Regulatory Agenda and the Addressing Air Pollution Horizontal Initiative. The work was also supported by AllerGen NCE, the British Columbia Lung Association, the Canada Research Chairs program, the Canadian Institutes of Health Research, Mitacs (through the Mitacs-Accelerate program), and the Michael Smith Foundation for Health Research. The authors thank G. Mallach and P. Blagden for helpful comments during presubmission review of the manuscript.