Synthesis, X-ray Single-Crystal Analysis, and Anticancer Activity Evaluation of New Alkylsulfanyl-Pyridazino[4,5-b]indole Compounds as Multitarget Inhibitors of EGFR and Its Downstream PI3K-AKT Pathway

Abstract

The alkylation of 3,5-dihydro-4H-pyridazino[4,5-b]indole-4-thione with benzyl bromide, ethyl chloroacetate, and allyl bromide in the presence of potassium carbonate (K₂CO₃) yielded new alkylsulfanylpyridazino[4,5-b]indole derivatives (i.e., compounds 4–6). Hydrazinolysis of ester 6 resulted in hydrazide 7. The structure of compound 6 was verified by X-ray single-crystal analysis. Among the synthesized compounds, compound 6 exhibited the most promising cytotoxicity toward MCF-7 cells with an IC₅₀ value of 12 µM. It showed potential inhibition activity toward EGFR, PI3K, and AKT in MCF-7 cells, with 0.26-, 0.49-, and 0.31-fold reductions in concentration compared to an untreated control. Additionally, it showed apoptosis-inducing activity in MCF-7 cells (47.98-fold); overall apoptosis increased to 38.87% compared to 0.81% in the untreated control, which disrupted the cell cycle at pre-G1 and S phases. Moreover, compound 6 exhibited good binding affinities toward the tested proteins (EGFR, PI3K, and AKT) and had binding energies ranging from −15.87 to −24.87 Kcal/mol. It also formed good interactions with essential amino acids inside the binding sites. Hence, compound 6 is recommended as an anti-breast cancer chemotherapeutic due to its effects on the EGFR-PI3K-AKT pathway.

1. Introduction

One of the most prevalent diseases that attacks any organ or part of the body is cancer. It is characterized by the uncontrollable and irregular growth of cells [1,2]. Therefore, scientists and researchers prepare a variety of chemicals to either be used in the treatment of cancer, improve medicines that are used in its treatment, or reduce its side effects on human health.

Pyridazino[4,5-b]indole’s nucleus contains two heterocyclic compounds that interact with many receptors and are important to biological and metabolic processes within the body [3,4,5,6]. Because of their bioisosterism with γ- and β-carboline, these two heterocyclic compounds have a wide range of uses in medicinal chemistry.

Pyridazino[4,5-b]indole derivatives have therapeutic effects as they act as thromboxane A2 synthetase inhibitors [7], antihypertensive MAO inhibitors [8], antihistaminic and HIV-1 reverse transcriptase inhibitors, antiarrhythmics [9], phosphatidylinositol 3-kinase (PI3K) inhibitors [10], blood platelet aggregation inhibitors, inotropics [11], serotonin antagonists [12], and anxiolytics [13], and they have antimicrobial and antiproliferative effects in different cell types [14]. Pyridazino[4,5-b]indole derivatives also have inhibitory effects against cancer cells such as human neuroblastoma IMR-32 cell lines (Figure 1), hepatocellular carcinoma Huh-7, human primary glioblastoma U-87, prostate carcinoma PC3, colorectal adenocarcinoma Caco2, breast cancer cells MDA-MB-231, colorectal carcinoma HCT-116, one normal cell line (fibroblasts), lung carcinoid NCI-H727, and MCF 7; additionally, they inhibit enzymes in cancer cells [15,16,17,18,19]. Moreover, pyridazino[4,5-b]indole derivatives provide powerful therapeutic effects for different diseases related to acute and chronic inflammation [20], neuroinflammation, and neoplastic progression [21].

The resistance to drugs that target the epidermal growth factor receptor (EGFR), such as cetuximab, is a crucial hurdle in the research on effective therapies for neck and head squamous cell carcinoma. The phosphatidylinositol 3-kinase (PI3K)-Akt pathway was suggested as a contributor to the resistance of EGFR inhibitors because of its deep bond with the EGFR pathway. Understanding the function of each of the key proteins in this pathway is important for developing rational combination tactics that can overcome resistance [22].

Breast cancer is caused by a variety of factors (exogenous and endogenous), each of which has a different effect [23]. Due to its aggressive characteristics, invasive breast cancer is difficult to treat [24,25]. Breast cancer therapy is dependent on a mechanism that can be targeted by synthetic or natural substances that are approved as anticancer medicines [26,27]. Literature reviews [28,29,30] noted that many heterocyclic compounds have been created as anti-breast cancer therapies, such as pertuzumab, trastuzumab deruxtecan, trastuzumab emtansine, lapatinib, trastuzumab, and neratinib. There are four subtypes of tumors: luminal A, luminal B, human epidermal growth factor receptor 2 (HER2), and triple-negative breast cancer (TNBC). In this study, novel alkylated pyridazino[4,5-b]indole derivatives were synthesized, and their activity toward breast cancer cell lines was evaluated.

2. Materials and Methods

2.1. General Procedure

Melting points were measured with the use of SMP10 apparatus in open capillaries and were uncorrected. Thin-layer chromatography (TLC) and UV light were used for monitoring reaction progress. Proton and carbon nuclear magnetic resonance (¹H- and ¹³C-NMR) were detected in CDCl₃ and DMSO-d₆ and were recorded on Bruker AC 400 and 100 MHz spectrometers in tetramethylsilane as internal standard. Chemical shift values were evaluated in δ (ppm), and coupling constants values were set in Hz. Flash EA-1112 instrument was used for elemental analysis (CHN) determination. X-ray single crystallographic analysis was collected on a Rigaku Oxford Diffraction Supernova diffractometer using Cu Kα radiation.

2.2. Synthesis

2.2.1. Synthesis of 3,5-Dihydro-4H-Pyridazino[4,5-b]indol-4-One (2)

A solution of 1.0 mmol of ester 1 and 2.0 mmol of hydrazine hydrate in 5 mL ethanol was kept under reflux for 3 hours and left to reach room temperature. Crystals of substance 2 formed and were collected by filtration and purified by recrystallization from ethanol.

Yield: 75%, m.p. > 300 °C [Lit. [31,32] 324–326 °C]. ¹H NMR (400 MHz, DMSO-d₆): δ 7.33 (dd, 1 H, J 7.2, 7.6 Hz, H-8_Ind.); 7.53 (dd, 1 H, J 7.2, 8.0 Hz, H-7_Ind.); 7.63 (d, 1 H, J 8.4 Hz, H-6_Ind.); 8.16 (d, 1 H, J 8.0 Hz, H-9_Ind.); 8.74 (s, 1 H, CH_pyridazine); 12.66, 12.76 (2s, 2 H, NH_indole + NH_pyridazine). ¹³C NMR (100 MHz, DMSO-d₆): δ 113.45, 117.98, 121.26, 121.79, 121.89, 127.44, 132.14, 133.78, 139.39, 156.19 (see in Supplementary Materials). Elemental analysis calculated for [C₁₀H₇N₃O]: C, 64.86; H, 3.81; N, 22.69; O, 8.64 vs. found: C, 64.66; H, 3.91; N, 22.55.

2.2.2. Synthesis of 3,5-Dihydro-4H-Pyridazino[4,5-b]indole-4-Thione (3)

A 10.0 mmol mixture of pyridazino[4,5-b]indol-4-one (i.e., substance 2), 25 mL of dry pyridine, and (2.25 g, 10 mmol) P₂S₅ was refluxed for 6 h. It was then cooled and poured into 250 mL ice water. The generated precipitate 3 was then filtered and dried.

Yield: 79%, m.p. > 300 °C. ¹H NMR (400 MHz, DMSO-d₆): δ 7.37 (dd, 1 H, J 7.6, J 8 Hz); 7.59 (dd, 1 H, J 7.6, J 8 Hz); 7.75 (d, 1 H, J 8.0 Hz); 8.25 (d, 1 H, J 8.0 Hz); 9.19 (s, 1 H); 12.54 (s, 1 H, NH indole); 14.47 (s, 1 H, NH). ¹³C NMR (100 MHz, DMSO-d₆): δ 112.24, 113.80, 120.78, 122.40, 122.82, 128.59, 137.79, 140.56, 140.56, 140.81, 169.24 (see in Supplementary Materials). Elemental analysis calculated for [C₁₀H₇N₃S]: C, 59.68; H, 3.51; N, 20.88; S, 15.93 vs. found: C, 59.71; H, 3.55; N, 20.93; S, 15.88.

2.2.3. Alkylation of Pyridazino[4,5-b]indole-4-Thione (3)

Compound 3 (1.0 mmol) and 2.2 mmol of potassium carbonate were stirred in 10 mL acetone at room temperature for 1 h. Alkyl halide (benzyl bromide, allyl bromide, and ethyl chloroacetate) was then added drop by drop. Stirring was continued overnight, followed by removing of solvent under vacuum, and then addition of water. The solution was then filtered, and the precipitate was dried and recrystallized from ethanol to yield pure products 4–6.

• 4-(Benzylsulfanyl)-5H-Pyridazino[4,5-b]indole (4)

Yield: 65%, m.p. 254–255 °C. ¹H NMR (400 MHz, DMSO-d₆): δ 4.80 (s, 2 H, SCH₂); 7.24–7.41 (m, 4 H); 7.51 (d, 2H, J 7.2 Hz); 7.62 (ddd, 1 H, J 8.1, 8.0, 0.8 Hz); 7.70 (d, 1 H, J 8.2 Hz); 8.32 (d, 1 H, J 8.0 Hz); 9.76 (s, 1 H); 12.34 (s, 1 H, NH_ind). ¹³C NMR (100 MHz, DMSO-d₆): δ 33.34 (SCH₂), 113.31, 117.31, 119.75, 121.84, 122.45, 127.68, 128.91, 129.09, 129.52, 138.22, 140.01, 142.68, 146.57 (see in Supplementary Materials). Elemental analysis calculated for [C₁₇H₁₃N₃S]: C, 70.08; H, 4.50; N, 14.42; S, 11.00 vs. found: C, 70.21; H, 4.59; N, 14.44; S, 11.08.

• 4-(Allylsulfanyl)-5H-Pyridazino[4,5-b]indole (5)

Yield: 71%, m.p. 252-253 °C. ¹H NMR (400 MHz, DMSO-d₆): δ 4.20 (d, 2 H, J 6.4 Hz, SCH₂); 5.14 (d, 1 H, J_cis 10.0 Hz, H_cis); 5.36 (d, 1 H, J_trans 17.2 Hz, H_trans); 6.03–6.13 (m, 1 H, -CH=CH₂); 7.39 (dd, 1 H, J 7.2, 7.6 Hz); 7.63 (dd, 1 H, J 7.2, 8.0 Hz); 7.71 (d, 1 H, J 8.0 Hz); 8.31 (d, 1 H, J 7.6 Hz); 9.74 (s, 1 H); 12.32 (s, 1 H, NH_ind). ¹³C NMR (100 MHz, DMSO-d₆): δ 32.22 (SCH₂), 113.34, 117.34, 118.61, 119.80, 121.81, 122.41, 129.05, 134.10, 134.26, 140.11, 142.55, 146.27 (see in Supplementary Materials). Elemental analysis calculated for [C₁₃H₁₁N₃S]: C, 64.71; H, 4.59; N, 17.41; S, 13.29 vs. found: C, 64.59; H, 4.63; N, 17.33; S, 13.31.

• Ethyl 2-((5H-Pyridazino[4,5-b]indol-4-yl)sulfanyl)acetate (6)

Yield: 83%, m.p. 218–219 °C. ¹H NMR (600 MHz, DMSO-d₆): δ 1.19 (t, 3 H, J 7.1 Hz, CH₃); 4.14 (q, 2 H, J 7.1 Hz, CH₂); 4.40 (s, 2 H, SCH₂); 7.41 (dd, 1 H, J 7.3, 7.7 Hz); 7.65 (dd, 1 H, J 7.4, 7.9 Hz); 7.72 (d, 1 H, J 8.2 Hz); 8.33 (d, 1 H, J 8.0 Hz); 9.74 (s, 1 H); 12.48 (s, 1 H, NH_ind). ¹³C NMR (150 MHz, DMSO-d₆): δ 14.5 (CH₃), 31.76 (SCH₂), 61.55 (OCH₂), 113.33, 117.34, 121.90, 122.51, 129.18, 133.57, 139.06, 142.76, 145.50, 169.09 (C=O) (see in Supplementary Materials). Elemental analysis calculated for [C₁₄H₁₃N₃O₂S]: C, 58.52; H, 4.56; N, 14.62; O, 11.14; S, 11.16 vs. found: C, 58.67; H, 4.59; N, 14.53; S, 11.22.

2.2.4. Hydrazinolysis of Ester 6

Ester 6 (1.0 mmol) and 2.0 mmol of hydrazine hydrate in 10 mL of ethanol were refluxed for 2 h and left to cool. Crystals formed and were collected by filtration, dried, and purified through recrystallization from ethanol.

• 2-((5H-Pyridazino[4,5-b]indol-4-yl)thio)acetohydrazide (7)

Yield: 80%, m.p. 241–242 °C. ¹H NMR (400 MHz, DMSO-d₆): δ 4.25 (s, 2 H, SCH₂); 4.35 (brs, 2 H, NH₂); 7.40 (dd, 1 H, J 7.2, 7.6 Hz); 7.63 (dd, 1 H, J 7.4, 7.9 Hz); 7.71 (d, 1 H, J 8.4 Hz); 8.31 (d, 1 H, J 8.0 Hz); 9.42 (brs, 1 H, NH); 9.74 (s, 1 H); 12.44 (s, 1 H, NH_ind). ¹³C NMR (100 MHz, DMSO-d₆): δ 31.95 (SCH₂), 113.34, 117.30, 119.75, 121.84, 122.42, 129.09, 133.77, 140.12, 142.62, 146.42, 167.34 (C=O) (see in Supplementary Materials). Elemental analysis calculated for [C₁₂H₁₁N₅OS]: C, 52.73; H, 4.06; N, 25.62; O, 5.85; S, 11.73 vs. found: C, 52.83; H, 4.05; N, 25.52; S, 11.70.

2.3. Biology

2.3.1. Cytotoxicity

MCF-7 and normal breast MCF-10A cell lines were collected from National Cancer Institute in Cairo, A.R.E, and grown in RPMI-1640 medium L-glutamine. The cells were grown in 10% fetal bovine serum (FBS) and 1% penicillin–streptomycin. All samples were cultured at 37 degrees Celsius in a 5% CO₂ gas. On the second day, cells were grown in triplicate on a 96-well plate at a density of 5 × 10⁴ cells and incubated with the investigated compounds at 0.01, 0.1, 1, 10, and 100 mM. MTT solution was used to determine cell viability. The plate was cultured for 3 hours. An ELISA microplate reader was used to measure the absorbance. The viability was determined by comparing it to the control group, and the IC50 values were recorded [31].

2.3.2. Enzymatic Targeting

To assess the inhibitory potency of compound 6 against the EGFR, an EGFR test kit (ADP-GloTM kinase assay, Cat No. V9261, Promega, Madison, WI, USA), a PI3K assay kit (Cat No. MBS268899, USA), and an AKT assay kit (Catalog #: MBS9511022, USA) were used. The percentage inhibition of autophosphorylation by substances was estimated using the following equation [32]:

(1)$\mathrm{Percentage} \mathrm{inhibition}=100-[\frac{Control}{Treated}-Control]$

2.3.3. Apoptosis Investigation

MCF-7 cells were incubated overnight in 6-well culture plates (3–5 × 10⁵ cells per well) and then treated for 48 h with compound 7. Afterward, the cells were incubated in a 100 µL solution of Annexin binding buffer (25 mM CaCl₂, 1.4 M NaCl, and 0.1 M Hepes–NaOH, pH 7.4) in the dark for 30 min with Annexin V-FITC solution (1:100) and propidium iodide (PI) at a concentration equivalent to 10 g/mL. The labeled cells were then extracted using the Cytoflex FACS machine. CytExpert software was used to analyze the data [33].

2.4. Molecular Docking

The investigated compounds were docked against the protein structures of EGFR (PDB = 1M17), PI3K (PDB = 5JHB), and AKT (PDB = 3KQQ) using MOE-2019 software. MOE-2019 was used to improve protein and ligand structures and to favor them energetically. Binding activities interpreted molecular docking results in terms of binding energy and ligand–receptor interactions. Visualization was then conducted with Chimera.

3. Results and Discussion

3.1. Chemistry

The reaction of hydrazine hydrate with ethyl 3-formyl-1H-indole-2-carboxylate (i.e., ester 1) in ethanol afforded 3,5-dihydro-4H-pyridazino[4,5-b]indol-4-one (i.e., substance 2). Thionation of substance 2 to a thione (i.e., compound 3) was conducted using P₂S₅ in pyridine [34,35]. The structure of compound 3 was deduced from ¹H and ¹³C NMR spectra, which showed an indole NH signal at 12.54 ppm and a pyridazine NH signal at 14.47 ppm. A thione tautomer was deduced from a thiocarbonyl (C=S) signal at 169.24 ppm. Alkylation of thionated compound 3 with benzyl bromide, allyl bromide, and ethyl chloroacetate was achieved in the presence of potassium carbonate and acetone. S-alkylation was confirmed from ¹³C NMR signals found at 33.34, 32.22, and 31.76 ppm in alkylated products 4–6, respectively (Scheme 1).

The reaction of hydrazine hydrate with ester 6 in ethanol led to the conversion of the ester group to hydrazide and the formation of product 7. The NMR of the hydrazide showed hydrazide group (-CONHNH₂) proton signals at 4.35 and 9.42 ppm and a carbonyl signal at 167.34 ppm (Scheme 2).

3.2. X-ray Single-Crystal Analysis and Structural Determinations

A single crystal of compound 6 was grown via the slow evaporation of ethanol. Crystal data showed that the compound had an empirical formula of C₁₄H₁₃N₃O₂S and crystallized in an orthorhombic crystal system (Pccn space group) (Figure 2). Molecules were stabilized in a 3D molecular packing network by strong, classical intermolecular hydrogen bonding, and the most important bond was N(1)-H(1)…N(2) (Figure 3).

The crystallographic details are summarized in Table 1.

3.3. Biology

3.3.1. Cytotoxic Activity

The cytotoxic activity of the synthesized compounds was tested against the breast MCF-7 cancer cell line. The results illustrated in Table 2 revealed that compounds 2, 3, and 7 showed weak activity with IC₅₀’s of 37.5, 94.3, and 39.9 μM, while compounds 1, 4, and 6 showed promising cytotoxic activity, especially the latter, which had an IC₅₀ value of 12 μM. Therefore, compound 6 was further screened for enzymatic targeting. Additionally, compound 6 was tested against normal breast MCF-10A cells for safety. Compared with Staurosporin, which had an IC₅₀ value of 21.43 μM, compound 6 exhibited poor cytotoxic activity against normal cells with an IC₅₀ value of 75.13 μM. Hence, compound 6 was selective in cytotoxicity against MCF-7 cells.

3.3.2. Enzymatic Targeting

The EGFR-PI3K-AKT-mTOR signaling cascade is involved in a variety of cell activities that contribute to tumor progression, including cell growth, proliferation, survival, metabolism, apoptosis, and differentiation [36]. To investigate effective molecular targets of cytotoxic activity, compound 6, which had the highest cytotoxic activity toward MCF-7 cells, was tested against EGFR, PI3K, and AKT inhibition.

As seen in Table 3, in the presence of compound 6, EGFR concentrations decreased 0.26-fold from 730 pg/mL to 191 pg/mL. In addition, compound 6 reduced PI3K concentrations, compared with untreated controls, from 6.64 ng/mL to 3.28 ng/mL, which was a 0.49-fold reduction. Furthermore, AKT concentrations were reduced 0.31-fold from 45.39 ng/mL to 14.06 pg/mL when compound 6 was added. Our PI3K inhibitory activity results are equivalent with Nafie et al. [37], who showed that a novel pyridazino[4,5-b]indole scaffold may be utilized as a PI3K inhibitor in the treatment of breast cancer.

3.3.3. Apoptotic Investigation

• Annexin V/PI Staining

To study the apoptotic and necrotic activity of the investigated compound, flow cytometric examinations of Annexin V/PI staining in treated and untreated MCF-7 cells with compound 6 (IC₅₀ = 12 mM, 48 h) were conducted.

Compound 6 enhanced overall apoptosis by 38.87% (13.81% for late and 25.06% for early) compared to 0.81% (0.15% for late and 0.66% for early) for the control, as shown in Figure 4. This compound also enhanced necrotic breast cancer cell death 5.39-fold; necrotic cell death was enhanced by 7.45% compared to 1.38%. As a result, apoptosis was adopted over necrosis as the mode of action.

• Cell Cycle Analysis

Cell cycle analysis was used to detect at which phase cells were arrested in both treated and untreated MCF-7 cells for the purpose of investigating the cell population percentage with cytotoxic chemicals after treatment in each cell phase. Figure 5 shows that the compound treatment increased the cell population by 46.32% in pre-G phase compared to 2.19% in the control and by 46.12% in S phase compared to 33.61% in the control, while the cell population was not significantly decreased. These findings are consistent with previous studies [38], which evaluated apoptosis-inducing activity using flow cytometric apoptosis and cell cycle analyses.

3.4. Molecular Docking

To investigate the mechanism behind compound 6’s promising cytotoxic and inhibitory activities toward EGFR, PI3K, and AKT, a study of molecular docking was conducted for these three proteins. Compound 6 exhibited good binding affinities toward the tested proteins of EGFR, PI3K, and AKT, and the binding energies ranged from −15.87 to −24.87 Kcal/mol. In addition, it formed good interactions with the key amino acids inside the bindings sites; hence, it maintained the binding interaction as the ligands cocrystallized (Figure 6). Compound 6 formed one hydrogen bond interaction with Met 769 inside the EGFR binding site. It made one hydrogen bond with Val 882 inside the PI3K binding site and one hydrogen bond with Asp 292 and arene–cation interactions with Lys 179 inside the AKT binding site. These findings of inhibiting EGFR, PI3K, and AKT agreed with previous studies using molecular docking approaches.

4. Conclusions

New alkylsulfanylpyridazino[4,5-b]indole compounds, i.e., compounds 4–6, were synthesized. A reaction involving a new alkylsulfanyl ester, i.e., ester 6, afforded the synthesis of hydrazide 7. Among the synthesized compounds, compound 6 exhibited the most promising cytotoxicity toward MCF-7 cells with an IC₅₀ value of 12 µM. Moreover, it exhibited promising inhibition activity toward EGFR and its downstream PI3K–AKT pathway, which suggests that it is a multitarget compound. Additionally, it increased apoptosis 47.98-fold in MCF-7 cells and increased total apoptosis by 38.87%. Hence, compound 6 is recommended as an anti-breast cancer chemotherapeutic due to its effects on the EGFR-PI3K-AKT pathway.

Author Contributions

Conceptualization, E.E.S. and A.T.A.B.; data curation, M.H.; formal analysis, M.H.; investigation, A.T.A.B. and E.E.S.; methodology, E.E.S. and A.T.A.B.; software, M.H.; visualization, M.H.; funding acquisition, E.E.S. and I.O.A.; writing—original draft, A.T.A.B., E.E.S., I.O.A. and M.H. All authors have read and agreed to the published version of the manuscript.

Funding

This research was funded by the Deanship of Scientific Research at Jouf University, K.S.A., grant number No. (DSR2020-04-1536).

Data Availability Statement

The data presented in this study are available on request from the corresponding author.

Acknowledgments

The authors extend their appreciation to the Deanship of Scientific Research at Jouf University for funding this work through research grant No. (DSR2020-04-1536).

Conflicts of Interest

The authors declare no conflict of interest.

Publisher’s Note: MDPI stays neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Figures, Schemes and Tables

Figure 1. Bioactive pyridazino[4,5-b]indole.

Scheme 1. Synthesis and alkylation of 3,5-dihydro-4H-pyridazino[4,5-b]indole-4-thione (i.e., compound 3).

Scheme 2. Hydrazinolysis of ester 6.

Figure 2. ORTEP projection of compound 6 (CCDC 2133074, see in Supplementary Materials).

Figure 3. Packing diagram of compound 6.

Figure 4. Cryptographs of Annexin V/Propidium Iodide staining of (left) untreated and (right) 6-treated MCF-7 cells with IC50 values, 48 h. Q1-UL (necrosis, AV−/PI+), Q2-UR (late apoptotic cells, AV+/PI+), Q3-LL (normal cells, AV−/PI−), and Q4-LR (early apoptotic cells, AV+/PI−).

Figure 5. Histograms of DNA content using flow-cytometry-aided cell cycle analyses of (A) untreated and (B) 6-treated MCF-7 cells (IC50 = 12 μM, 48 h), with the percentage of cell population at G1, S, and G2/M phases.

Figure 6. Binding disposition and ligand–receptor interactions between the cocrystallized ligand (green-colored) and docked compound 6 (cyan-colored) inside the three proteins of EGFR (PDB = 1M17), PI3K (5JHB), and AKT (PDB = 3QKK). The 3D images were obtained using Chimera. Compound 6 and the cocrystallized ligands are compared for highlighting the interaction binding mode inside the protein active sites and the lipophilic interactions with lipophilic amino acids.

Table 1

Crystal data set of target molecule 6.

Compound	6
CCDC	2133074
Empirical formula	C14H13N3O2S
FW	287.33
Temp (K)	120 (2)
λ (Å)	1.54184
Crystal system	Orthorhombic
Space group	Pccn
a (Å)	23.7290 (2)
b (Å)	13.06630 (10)
c (Å)	8.45260 (10)
V (Å3)	2620.73 (4)
Z	8
ρcalc (Mg/m3)	1.456
μ(Mo Kα) (mm−1)	2.248
No. reflns.	31606
Unique reflns.	2768
Completeness to θ = 67.684°	100.0%
GOOF (F2)	1.038
Rint	0.0335
R1 a (I ≥ 2σ)	0.0284
wR2 b (I ≥ 2σ)	0.0764

^aR1 = Σ||F_o| − |F_c||/Σ|F_o|. ^b wR₂ = {Σ[w(F_o² − F_c²)²]/Σ[w(F_o²)²]}^1/2.

Table 2

IC₅₀ values of the tested compounds against MCF-7 lines using the MTT assay.

Compound	IC50 ± SD *,# (µM)/MCF7	IC50 ± SD *,# (µM)/MCF-10A
(1)	19.7 ± 2.31	-
(2)	37.5 ± 1.95	-
(3)	94.3 ± 4.9	-
(4)	20.4 ± 1.06	-
(6)	12.0 ± 0.63	75.13 ± 1.87
(7)	39.9 ± 2.07	-
Staurosporin	8.32 ± 0.43	21.43 ± 1.06

* Values are expressed as mean ± SD of three independent triplets (n = 3). ^# A nonlinear regression dose–inhibition curve was used to calculate IC₅₀ values in Excel.

Table 3

Activity of tested compound 6 against EGFR, PI3K, and AKT pathways in MCF-7 cells.

Compound	EGFR (pg/mL) *	PI3K(ng/mL) *	AKT(ng/mL) *
Control	730 ± 62.2	6.638 ± 0.28	45.39 ± 3.74
Treated with 6	191.3 ± 7.3	3.28 ± 0.09	14.06 ± 0.5

* Values are expressed as mean ± SD of three independent replicates.

Supplementary Materials

The following supporting information can be downloaded at https://www.mdpi.com/article/10.3390/cryst12030353/s1, Cif file of compound 6; Figure S1: ¹H NMR of 2; Figure S2: ¹³C NMR of 2; Figure S3: ¹H NMR of 3; Figure S4; ¹³C NMR of 3; Figure S5: ¹H NMR of 4; Figure S6: ¹³C NMR of 4; Figure S7: ¹H NMR of 5; Figure S8: ¹³C NMR of 5; Figure S9: ¹H NMR of 6; Figure S10: ¹³C NMR of 6; Figure S11: ¹H NMR of 7; Figure S12: ¹³C NMR of 7.

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