Abstract

Light-sheet fluorescent microscopy has become the leading technique for in vivo imaging in the fields of disease, medicine, and cell biology research. However, designing proper illumination for high image resolution and optical sectioning is challenging. Another issue is geometric constraints arising from the multiple bulky components for illumination and detection. Here, we demonstrate that those issues can be well addressed by integrating nanophotonic meta-lens as the illumination component for LSFM. The meta-lens is composed of 800-nm-thick GaN nanostructures and is designed for a light-sheet well-adapted to biological specimens such as the nematode Caenorhabditis elegans (C. elegans). With the meta-lens, the complexity of the LSFM system is significantly reduced, and it is capable of performing multicolor fluorescent imaging of live C. elegans with cellular resolution. Considering the miniature size and plane geometry of the meta-lens, our system enables a new design for LSFM to acquire in vivo images of biological specimens with high resolution.

Details

Title
Meta-lens light-sheet fluorescence microscopy for in vivo imaging
Author
Luo, Yuan 1 ; Tseng, Ming Lun 2 ; Vyas, Sunil 1 ; Ting-Yu, Hsieh 1 ; Wu, Jui-Ching 3 ; Shang-Yang, Chen 1 ; Hsiao-Fang, Peng 1 ; Su, Vin-Cent 4 ; Huang, Tzu-Ting 5 ; Hsin Yu Kuo 1 ; Chu, Cheng Hung 1 ; Mu Ku Chen 6   VIAFID ORCID Logo  ; Jia-Wern Chen 1 ; Yu-Chun, Chen 1 ; Huang, Kuang-Yuh 5 ; Chieh-Hsiung Kuan 1 ; Xu, Shi 7 ; Misawa, Hiroaki 7 ; Tsai, Din Ping 8   VIAFID ORCID Logo 

1  National Taiwan University, Institute of Medical Device and Imaging, No. 1 Ren Ai Rd. Sect. 1, Taipei, 10051, Taiwan, ROC 
2  Institute of Electronics, National Yang Ming Chiao Tung University, Hsinchu, Taiwan, ROC 
3  Department of Clinical Laboratory Sciences and Medical Biotechnology, National Taiwan University, No. 1., Chang-Te St., Taipei, 100, Taiwan, ROC 
4  Electrical Engineering, National United University, No. 2, Lienda, Miaoli, 36003, Taiwan, ROC 
5  Mechanical Engineering, National Taiwan University, No. 1, Sec. 4, Roosevelt Rd., Taipei, 10617, Taiwan, ROC 
6  Department of Electrical Engineering, City University of Hong Kong, Kowloon, Hong Kong 
7  Hokkaido University, Sapporo, Hokkaido, Japan 
8  Department of Electrical Engineering, The Hong Kong Polytechnic University, City University of Hong Kong, Kowloon, Hong Kong 
Pages
1949-1959
Publication year
2022
Publication date
2022
Publisher
Walter de Gruyter GmbH
ISSN
21928606
e-ISSN
21928614
Source type
Scholarly Journal
Language of publication
English
DOI
https://doi.org/10.1515/nanoph-2021-0748
ProQuest document ID
2661579996
Copyright
© 2022. This work is published under http://creativecommons.org/licenses/by/4.0 (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.