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© 2022 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.

Abstract

Metalloendopeptidase ADAM-Like Decysin 1 (ADAMDEC1) is an anti-inflammatory peptidase that is almost exclusively expressed in the gastrointestinal (GI) tract. We have recently found abundant and selective expression of Adamdec1 in colonic mucosal PDGFRα+ cells. However, the cellular origin for this gene expression is controversial as it is also known to be expressed in intestinal macrophages. We found that Adamdec1 mRNAs were selectively expressed in colonic mucosal subepithelial PDGFRα+ cells. ADAMDEC1 protein was mainly released from PDGFRα+ cells and accumulated in the mucosal layer lamina propria space near the epithelial basement membrane. PDGFRα+ cells significantly overexpressed Adamdec1 mRNAs and protein in DSS-induced colitis mice. Adamdec1 was predominantly expressed in CD45 PDGFRα+ cells in DSS-induced colitis mice, with only minimal expression in CD45+ CD64+ macrophages. Additionally, overexpression of both ADAMDEC1 mRNA and protein was consistently observed in PDGFRα+ cells, but not in CD64+ macrophages found in human colonic mucosal tissue affected by Crohn’s disease. In summary, PDGFRα+ cells selectively express ADAMDEC1, which is localized to the colon mucosa layer. ADAMDEC1 expression significantly increases in DSS-induced colitis affected mice and Crohn’s disease affected human tissue, suggesting that this gene can serve as a diagnostic and/or therapeutic target for intestinal inflammation and Crohn’s disease.

Details

Title
Metalloendopeptidase ADAM-like Decysin 1 (ADAMDEC1) in Colonic Subepithelial PDGFRα+ Cells Is a New Marker for Inflammatory Bowel Disease
Author
Ha, Se Eun 1   VIAFID ORCID Logo  ; Jorgensen, Brian G 1 ; Lai, Wei 1 ; Jin, Byungchang 1 ; Kim, Min-Seob 2 ; Poudrier, Sandra M 1 ; Singh, Rajan 1   VIAFID ORCID Logo  ; Bartlett, Allison 1   VIAFID ORCID Logo  ; Zogg, Hannah 1   VIAFID ORCID Logo  ; Kim, Sei 1 ; Baek, Gain 1 ; Kurahashi, Masaaki 3 ; Moon-Young, Lee 2   VIAFID ORCID Logo  ; Yong-Sung, Kim 2   VIAFID ORCID Logo  ; Choi, Suck-Chei 4 ; Sasse, Kent C 5 ; Rubin, Samuel J S 6   VIAFID ORCID Logo  ; Gottfried-Blackmore, Andres 6   VIAFID ORCID Logo  ; Becker, Laren 6 ; Habtezion, Aida 6 ; Sanders, Kenton M 1 ; Ro, Seungil 1   VIAFID ORCID Logo 

 Department of Physiology and Cell Biology, University of Nevada School of Medicine, Reno, NV 89557, USA; [email protected] (S.E.H.); [email protected] (B.G.J.); [email protected] (L.W.); [email protected] (B.J.); [email protected] (S.M.P.); [email protected] (R.S.); [email protected] (A.B.); [email protected] (H.Z.); [email protected] (S.K.); [email protected] (G.B.); [email protected] (K.M.S.) 
 Department of Physiology, Wonkwang Digestive Disease Research Institute and Institute of Wonkwang Medical Science, School of Medicine, Wonkwang University, Iksan 54538, Korea; [email protected] (M.-S.K.); [email protected] (M.-Y.L.); [email protected] (Y.-S.K.) 
 Department of Internal Medicine, Division of Gastroenterology and Hepatology, University of Iowa, Iowa City, IA 52242, USA; [email protected] 
 Department of gastroenterology, Wonkwang Digestive Disease Research Institute and Institute of Wonkwang Medical Science, School of Medicine, Wonkwang University, Iksan 54538, Korea; [email protected] 
 Sasse Surgical Associates, Reno, NV 89502, USA; [email protected] 
 Gastroenterology and Hepatology, Stanford University School of Medicine, Stanford, CA 94305, USA; [email protected] (S.J.S.R.); [email protected] (A.G.-B.); [email protected] (L.B.); [email protected] (A.H.) 
First page
5007
Publication year
2022
Publication date
2022
Publisher
MDPI AG
ISSN
16616596
e-ISSN
14220067
Source type
Scholarly Journal
Language of publication
English
ProQuest document ID
2663057201
Copyright
© 2022 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.