Abstract

Background

Methylation of the promoter at CpG islands is a mechanism of silencing tumor suppressor genes and therefore enhances cancer progression. The study aimed to examine promoter methylation frequencies of five tumor suppressor genes in hepatocellular carcinoma and their implication on the first-year outcome of surgical resection of the tumor. Fifty specimens of hepatocellular carcinoma and the adjacent non-tumorous liver tissue were collected from the surgically resected hepatic tumor. The status of promoter methylation of tumor suppressor genes RASSF1A, CHFR, MGMT, GSTP1, and hMLH1 was investigated using methylation-specific polymerase chain reaction.

Results

The frequency of promoter methylation of these tumor suppressors genes (TSG) genes in hepatocellular carcinoma was significantly higher than non-tumorous tissue all, P < 0.05, with a methylation rate of 80% in RASSF1A, 70% in CHFR, 46% in GSTP1, 56% in MGMT, and 10% in hMLH1. Methylation of RASSF1A, CHFR, and MGMT promoter genes was significantly associated with decreased first-year postoperative survival and increased recurrence of hepatocellular carcinoma, P < 0.05.

Conclusion

Methylated RASSF1A, CHRF, and MGMT promoters indicated poor prognosis among patients with hepatocellular carcinoma and may serve as potential prognostic indicators in patients with hepatocellular carcinoma.

Details

Title
Significance of promoter methylation of multiple tumor suppressor genes in hepatocellular carcinoma
Author
Tahoon, Alaa 1 ; El-Khateeb, Doaa 2 ; Mosbeh, Asmaa 3 ; Tantawy El Sayed, Ibrahim 1 ; Khalil, Ashraf 2   VIAFID ORCID Logo 

1  Menoufia University, Department of Biochemistry, Faculty of Science, Shebin Elkom, Egypt (GRID:grid.411775.1) (ISNI:0000 0004 0621 4712) 
2  National Liver Institute, Menouffia University, Department of Clinical Biochemistry and Molecular Diagnostics, Shebin Elkom, Egypt (GRID:grid.411775.1) (ISNI:0000 0004 0621 4712) 
3  National Liver Institute, Menouffia University, Department of Pathology, Shebin Elkom, Egypt (GRID:grid.411775.1) (ISNI:0000 0004 0621 4712) 
Pages
22
Publication year
2022
Publication date
Dec 2022
Publisher
Springer Nature B.V.
ISSN
11108630
e-ISSN
20902441
Source type
Scholarly Journal
Language of publication
English
DOI
https://doi.org/10.1186/s43042-022-00237-5
ProQuest document ID
2670525193
Copyright
© The Author(s) 2022. This work is published under http://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.