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© 2022 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.

Abstract

Extracorporeal shockwave therapy (ESWT) can stimulate processes to promote regeneration, including cell proliferation and modulation of inflammation. Specific miRNA expression panels have been established to define correlations with regulatory targets within these pathways. This study aims to investigate the influence of low-energy ESWT—applied within the subacute and chronic phase of SCI (spinal cord injury) on recovery in a rat spinal cord contusion model. Outcomes were evaluated by gait analysis, µCT and histological analysis of spinal cords. A panel of serum-derived miRNAs after SCI and after ESWT was investigated to identify injury-, regeneration- and treatment-associated expression patterns. Rats receiving ESWT showed significant improvement in motor function in both a subacute and a chronic experimental setting. This effect was not reflected in changes in morphology, µCT-parameters or histological markers after ESWT. Expression analysis of various miRNAs, however, revealed changes after SCI and ESWT, with increased miR-375, indicating a neuroprotective effect, and decreased miR-382-5p potentially improving neuroplasticity via its regulatory involvement with BDNF. We were able to demonstrate a functional improvement of ESWT-treated animals after SCI in a subacute and chronic setting. Furthermore, the identification of miR-375 and miR-382-5p could potentially provide new targets for therapeutic intervention in future studies.

Details

Title
Effects of Extracorporeal Shockwave Therapy on Functional Recovery and Circulating miR-375 and miR-382-5p after Subacute and Chronic Spinal Cord Contusion Injury in Rats
Author
Ashmwe, Mohamed 1 ; Posa, Katja 1 ; Rührnößl, Alexander 1 ; Heinzel, Johannes Christoph 2 ; Heimel, Patrick 3   VIAFID ORCID Logo  ; Mock, Michael 1 ; Schädl, Barbara 4   VIAFID ORCID Logo  ; Keibl, Claudia 1 ; Couillard-Despres, Sebastien 5   VIAFID ORCID Logo  ; Redl, Heinz 1 ; Mittermayr, Rainer 1   VIAFID ORCID Logo  ; Hercher, David 6   VIAFID ORCID Logo 

 The Research Center in Cooperation with AUVA, Ludwig Boltzmann Institute for Traumatology, 1200 Vienna, Austria; [email protected] (M.A.); [email protected] (K.P.); [email protected] (A.R.); [email protected] (P.H.); [email protected] (M.M.); [email protected] (B.S.); [email protected] (C.K.); [email protected] (H.R.); [email protected] (R.M.); Austrian Cluster for Tissue Regeneration, 1200 Vienna, Austria; [email protected] 
 Department of Hand, Plastic, Reconstructive and Burn Surgery, University of Tuebingen, 72074 Tübingen, Germany; [email protected] 
 The Research Center in Cooperation with AUVA, Ludwig Boltzmann Institute for Traumatology, 1200 Vienna, Austria; [email protected] (M.A.); [email protected] (K.P.); [email protected] (A.R.); [email protected] (P.H.); [email protected] (M.M.); [email protected] (B.S.); [email protected] (C.K.); [email protected] (H.R.); [email protected] (R.M.); Austrian Cluster for Tissue Regeneration, 1200 Vienna, Austria; [email protected]; Core Facility Morphology, University Clinic of Dentistry, Medical University of Vienna, 1090 Vienna, Austria 
 The Research Center in Cooperation with AUVA, Ludwig Boltzmann Institute for Traumatology, 1200 Vienna, Austria; [email protected] (M.A.); [email protected] (K.P.); [email protected] (A.R.); [email protected] (P.H.); [email protected] (M.M.); [email protected] (B.S.); [email protected] (C.K.); [email protected] (H.R.); [email protected] (R.M.); Austrian Cluster for Tissue Regeneration, 1200 Vienna, Austria; [email protected]; Institute of Experimental Neuroregeneration, Spinal Cord Injury & Tissue Regeneration Centre Salzburg (SCI-TreCS), Paracelsus Medical University, 5020 Salzburg, Austria 
 Austrian Cluster for Tissue Regeneration, 1200 Vienna, Austria; [email protected]; Institute of Experimental Neuroregeneration, Spinal Cord Injury & Tissue Regeneration Centre Salzburg (SCI-TreCS), Paracelsus Medical University, 5020 Salzburg, Austria 
 The Research Center in Cooperation with AUVA, Ludwig Boltzmann Institute for Traumatology, 1200 Vienna, Austria; [email protected] (M.A.); [email protected] (K.P.); [email protected] (A.R.); [email protected] (P.H.); [email protected] (M.M.); [email protected] (B.S.); [email protected] (C.K.); [email protected] (H.R.); [email protected] (R.M.); Austrian Cluster for Tissue Regeneration, 1200 Vienna, Austria; [email protected]; Department Life Science Engineering, University of Applied Sciences Technikum Wien, 1200 Vienna, Austria 
First page
1630
Publication year
2022
Publication date
2022
Publisher
MDPI AG
e-ISSN
22279059
Source type
Scholarly Journal
Language of publication
English
ProQuest document ID
2693933450
Copyright
© 2022 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.