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Abstract
2022 In Vitro Biology Meeting, June 4 - 7, San Diego, California Late Submission Abstracts PLANT SYMPOSIA ABSTRACTS ADVANCES IN CANNABILS TECHNOLOGY Tuesday, June , 10:30 am - 12:30 pm 10:35 P-16 Controlled Ex Vitro Environments to Root Cannabis Microcuttings Following Multiple Harvest in a Hedging System Jeffrey Adelberg, Clemson University 11:50 P-19 Cannabis Virology in 2022 Diagnostic and Remediation Options Joseph Ramahi, Ploidy LLC PLANT CONTRIBUTED PAPER SESSION ABSTRACTS BIOTECHNOLOGY, GENOME EDITING, AND GENETIC ENGINEERING CONTRIBUTED PAPER SESSION Monday, June 6, 5:00 pm - 6:00 pm 5:00 P-1015 Molecular Physiological Responses of Paulownia and Turmeric Under Various Abiotic Stress Conditions C. Basu, California State University, Northridge, M. Chaires, K. Cooper, D. Gupta, N. Joshee, N. Katiyar, S. Pakala, N. Ramadoss, B. Bharadwaj, and K. Musaev 5:15 P-1016 DNA-Free Genome Editing in Hexaploid Sweetpotato Directed by Preassembled CRISPR-Cas9 Ribonucleoprotein Complexes Adrianne Brown, Tuskegee University Plant Biotechnology and Genomics Research Laboratory, M. Egnin, F. Bukari, D. Mortley, C. Bonsi, O. Idehen, D. Alexander, and G. Bernard 5:30 P-1017 Constitutive Expression of a miR169 Gene Alters Plant Development and Enhances Drought and Salt Tolerance in Transgenic Creeping Bentgrass Xiaotong Chen, Clemson University, Jason Yeung, Andrew Fiorentino, Qian Hu, Morgan Kuess, and Hong Luo 5:45 P-1018 T-DNA-Free Gene Editing Through Transient Suppression of the POLQ Gene in Plants Heqiang Huo, University of Florida, Guiluan Wang, and Zhanao Deng PLANT POSTER ABSTRACTS INTERACTIVE POSTERS BIOTECHNOLOGY, GENOMICS, AND PLANT PHYSIOLOGY INTERACTIVE POSTER SESSION Monday, June 6, 1:30 pm - 2:30 pm P-3000 A Rapid and Simplified Transformation and Genome Editing Method for Maize Inbred B104 Using Agrobacterium Ternary Vector System and Immature Embryos Minjeong Kang, Iowa State University, Keunsub Lee, Todd Finley, Hal Chappell, Veena Veena, and Kan Wang P-3001 CRISPR/Cas9 Mediated Cell Wall Engineering of Plant Cells for Enhanced Recombinant Protein Production Uddhab Karki, Arkansas State University, Hong Fang, and Jianfeng Xu P-3002 Post-wildfire Soil Microbiome Analysis of eDNA from the Angeles National Forest Savanah Senn, Los Angeles Pierce College, Gerald Presley, and Sharmodeep Bhattacharyya P-3003 Advanced Plant Reporter Genes for Transient Expression Nathan Vorodi, Pennsylvania State University, Natalie Thompson, David Samson, Rekha Kandaswamy, Vijay Sheri, Aliya F. Anwar, Michael Ream, Anna Filipkowski, Maia Clipsham, Sairam Rudrabhatla, and Wayne R. Curtis P-3004 Establishment of an Axenic Whitefly Colony for In Vitro Virus Transmission Studies Wayne R. Curtis\ The Pennsylvania State University, Natalie Thompson, David Krum, Yun-Ru Chen, Mariela Torres, Marena Trauger, Dalton Strike, Zach Weston, April Hile, and Jane Polston P-3005 Genome-wide Analysis of Alpha-amylase Gene Family in Major Cereal Crops Shabda Verma, Punjab Agricultural University, Kanwardeep S. Rawale, Navraj Kaur Sarao, Johar Singh Saini, Gagandeep Singla, and Kulvinder S Gill MICROPROPAGATION/MEDICINAL PLANTS INTERACTIVE POSTER SESSION Tuesday, June 7, 1:30 pm - 2:30 pm P-3006 Mutation Rates in Micropropagated Cannabis sativa Detected Through Genotyping by Sequencing, Are SSRs Antiquated? Genomotypes from the Rootzone of the Medicinal Plant Datura inoxia by Whole Genome Sequencing Savanah Senn, Los Angeles Pierce College, Kelly Pangell, Maryam Saraylou, Adrianna Bowerman, Karu Smith, and Ryan O'Halloran P-3010 Moringa oleífera Leaf Extract as a Potential Plant Growth Stimulant to Enhance Salt Stress Tolerance in Wheat Talaat Ahmed, Qatar University, Mustafa Elshiekh, and Muhammad Fasih Khalid GENETIC ENGINEERING AND METABOLIC ENGINEERING INTERACTIVE POSTER SESSION - Tuesday, June 7, 1:30 pm - 2:30 pm P-3012 Increased Engineering and Editing Efficiency of Sorghum bicolor Using Morphogene-assisted Transformation Kiflom Aregawi, University of California, Berkeley, Jianqiang Shen, Grady Pierroz, Manoj K. Sharma, Jeffery Dahlberg, Judith Owiti, and Peggy G. Lemaux P-3013 Inducible Expression for Acceleration of Design-Build-Test-Learn Cycles in the Metabolic Engineering of Oilcane Moni Qiande, University of Florida - IFAS, Dang Viet Cao, Hui Liu, John Shanklin, and Fredy Altpeter P-3014 Application of Developmental Regulators to Improve In-Planta or In Vitro Transformation in Plants Heqiang Huo, University of Florida, Zhaoyuan Lian, Chi Dinh Nguyen, Jianjun Chen, Sandra Wilson, and Peggy Ozias-Akins P-3016 Protein Engineering in Chlamydomonas reinhardtii for Improved Production of Recombinant Proteins Corbin England, Arkansas State University, and Jianfeng Xu P-3017 Development of an In Vitro Regeneration and Transformation System for Hop (Humulus lupulus) Christopher J. Willig, Oregon State University, Michele S. Wiseman, John A. Henning, and David H. Gent, and Steven H. Strauss POSTERS BIOTECHNOLOGY P-3018 Development of a Meristem-based Transformation System for Barley (Hordeum vulgare L.) Targeting Mature Embryo Explants Lucas Gontijo Silva Maia, University of Wisconsin-Madison, Edward Williams, Mike Petersen, Ray Collier, Phil Bregitzer, Ramamurthy Mahalingam, Marcus Vinje, Shawn Kaeppler, and Heidi Kaeppler P-3019 Construction of Wheat Transformation System Using GRF-GIF Chimera Gene Cloned from Korean Wheat cv. Keumgang and 'Speed Breeding' Conditions Geon Hee Lee, Kongju National University, Sang Yong Park, Tae Kyeum Kim, and Jae Yoon Kim P-3020 Effects of Organellar Targeting of the aadA1a Selectable Marker Protein on Transformation Frequency of Meristem-based Soybean Transformation S. Massman, University of Wisconsin Madison, R. Collier, E. Williams, M. Petersen, R. Harnish, B. Martinell, and H. Kaeppler P-3021 Optimizing Agrobacterium-mediated Transformation and CRISPR-Cas9 Gene Editing in the Tropical Japonica Rice Variety Presidio Marco Molina-Risco, Texas A&M University, Michael Thomson, Mayra Faion-Molina, Oneida Ibarra, Backki Kim, and Endang M. Septiningsih P-3022 A Dual Recombination System for Transgene Containment and Elimination in Perennial Grasses Xiaotong Chen, Clemson University, Charles Henry, Annalise Enger, Qian Hu, Zhenyuan Pan, Xiaoyuan Gao, Lynda McMaster-Schuyler, Peiyu Zeng, and Hong Luo P-3023 Transcriptome and Metabolite Mechanisms Related to Pre-harvest Sprout (PHS) of Common Korean Wheat (Triticum aestivum) Sang Yong Park, Kongju National University, Geon Hee Lee, Woo Joo Jung, and Jae Yoon Kim P-3024 Plant Host Defense Peptides: Potential Tools for Disease Control Christie Stephen, University of Lethbridge, and Dmytro P. Yevtushenko P-3025 Transforming P. hallii, a Model for Perennial Bioenergy Grasses Kankshita Swaminathan, HudsonAlpha Institute for Biotechnology, Anthony Trieu, Xiaoyu Weng, Rebekah Wood, and Thomas Juenger P-3026 Engineering Novel Designer Biologics in Plant Cells for Oral Treatment of Inflammatory Bowel Disease Jianfeng Xu, Arkansas State University, Wenzheng Guo, Jonathan TrejoMartinez, and Uddhab Karki P-3027 Chromosomal Aberrations and Micronuclei of Somaclonal Variation in Onion Tissue Culture (Allium cepa) Abdelrahem Yousef, Agriculture Research Centre, Giza, Abdel-Rahem T. Abdel-Rahem, Kasem Z. Ahmed, and Sayed Osman CELLULAR AND MOLECULAR BIOLOGY P-3028 Transcriptomic Analysis of Dunaliella Salina under Salt and UV Stresses M. J. Hein, California State University Northridge, C. Basu, and S. M. Perl P-3029 Genetic and Physiological Responses of Lentil Plants Under Flood Stress Avetis Mishegyan, California State University Northridge, Bhiolina Bharadwaj, Sanjeevi Nagalingam, Alex Guenther, Nirmal Joshee, Samantha H. Herman, and Chhandak Basu P-3030 Transcriptomic and Physiological Effects of Heat Stress on Turmeric (Curcuma longa) Kirill Musaev, California State University Northridge, and Chhandak Basu GENETIC ENGINEERING P-3031 Expanding the Toolbox of Maize Promoters Nathaniel Schleif, University of Wisconsin - Madison, Frank McFarland, Ray Collier, and Heidi F. Kaeppler P-3032 The Plant Genetic Engineering Network J. Van Eck, Boyce Thompson Institute, H. Kaeppler, B. Gordon-Kamm, K. Lee, W. Parrott, N. Taylor, and V Veena P-3049 Development of an Efficient Method for Protoplast Isolation, Transfection, and Gene Editing Form Soybean Roots C. L. Nwoko, Texas Tech University, A. Ojha, V. Devkar and Gunvant B. Patil GENOME EDITING/GENOME ENGINEERING P-3033 Synteny Analysis: Genes Linked for Powdery Mildew Among Landraces of Pisum sativum and Lathyrus sativus R. Bishnoi, Agriculture University, S. Marker, and P. K. P. Meena P-3034 Genome Editing in Three Species of the Bioenergy Grass Miscanthus Nancy A. Reichert, Mississippi State University, Anthony Trieu, Mohammad Belaffif, Shilpa Manjunatha, Pradeepa Hirannaiah, Rebekah Wood, Yokshitha Bathula, Rebecca Billingsley, Anjali Arpan, Erik Sacks, Stephen P. Moose, Tom E. Clemente, and Kankshita Swaminathan P-3035 Development of Tissue Culture-free Genetic Transformation and Gene-editing Platform in Crops Arjun Ojha Kshetry, Texas Tech University, Vikas Devkar, Luis Herrera-Estrella, and Gunvant B. Patil P-3036 Phenotypic Assessment of SnRK1C Mutants in Rice (Oryza sativa var. japonica) cv. Kitaake M. C. Faria Chaves, University of Arkansas, C. Maurya, N. Soumen, S. Zhao, and V. Srivastava P-3037 CRISPR RNA-guided Integrase System for Agrobacterium Genome Editing Ephraim Aliu, Iowa State University, Keunsub Lee, and Kan Wang P-3038 Transformation of Teosinte (Zea mays ssp. parviglumis) via Biolistic Bombardment of Seedling Derived Callus Tissues Mercy K. Azanu, Iowa State University, Jacob D. Zobrist, Susana Martin-Ortigosa, Keunsub Lee, Qing Ji, and Kan Wang IN VITRO TOOLS, TECHNIQUES, AND OPTIMIZATION P-3051 Production-scale Transformation of Poplar Hybrid Clone INRA 717-1B4, P. tremula x P. alba by Particle Bombardment for Enhanced Photosynthesis Traits Michelle Tjahjadi, Living Carbon, Alex Crites, Dominick Tucker, Kenneth Donsky, Gary Orr, Karli Rasmussen, Jessica Du, Li-Wei Chiu, and Matt Heckert MEDICINAL PLANTS P-3039 Physiological and Transcriptomic Analysis of Turmeric plants (Curcuma longa) Under Drought Stress Bhiolina Bharadwaj, California State University, Northridge, and Chhandak Basu