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© 2022. This work is published under http://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.

Abstract

Background

Although animal models of PCOS have been used in many studies, none of them can reproduce both the reproductive and metabolic phenotypes of PCOS. In addition, behavioral parameters have not been evaluated in PCOS animal models.

Purpose

We tried to produce an improved rat model of PCOS, and the reproductive, metabolic, and behavioral phenotypes of the model rats were evaluated.

Methods

Female rats were implanted with silicon tubes containing oil‐dissolved dihydrotestosterone (Oil‐DHT) as a new PCOS model. Their phenotypes were compared with those of conventional PCOS model rats (DHT), into which tubes containing crystalline DHT were implanted, and non‐DHT‐treated rats (control).

Results

Both the Oil‐DHT and DHT rats showed greater body weight gain, food intake, and fat depot weight than the control rats. Furthermore, these groups showed fewer estrous stages and increased numbers of cystic follicles. The DHT rats exhibited lower ovarian and uterine weights than the control rats, whereas no such changes were observed in the Oil‐DHT rats. The Oil‐DHT and DHT rats showed less locomotor activity in the light phase than the control rats.

Conclusions

Our proposed PCOS model reproduced both the reproductive and metabolic phenotypes of PCOS and may have potential for PCOS research.

Details

Title
A novel PCOS rat model and an evaluation of its reproductive, metabolic, and behavioral phenotypes
Author
Kamada, Shuhei 1 ; Yamamoto, Yuri 1 ; Aoki, Hidenori 1 ; Tamura, Kou 1 ; Takeda, Asuka 1 ; Minato, Saki 1 ; Masaki, Rie 1 ; Yanagihara, Rie 1 ; Hayashi, Noriko 1 ; Yano, Yuya 1 ; Imaizumi, Junki 1 ; Kagawa, Tomohiro 1 ; Yoshida, Atsuko 1 ; Kawakita, Takako 1   VIAFID ORCID Logo  ; Irahara, Minoru 1 ; Iwasa, Takeshi 1   VIAFID ORCID Logo 

 Department of Obstetrics and Gynecology, Graduate School of Biomedical Sciences, Tokushima University, Tokushima, Japan 
Section
ORIGINAL ARTICLES
Publication year
2022
Publication date
Jan/Dec 2022
Publisher
John Wiley & Sons, Inc.
ISSN
14455781
e-ISSN
14470578
Source type
Scholarly Journal
Language of publication
English
ProQuest document ID
2758333292
Copyright
© 2022. This work is published under http://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.