It appears you don't have support to open PDFs in this web browser. To view this file, Open with your PDF reader
Abstract
Nepal is an endemic country for dengue infection with rolling of every 3 year’s clear cyclic outbreaks with exponential growth since 2019 outbreak and the virus gearing towards the non-foci temperate hill regions. However, the information regarding circulating serotype and genotype is not frequent. This research discusses on the clinical features, diagnosis, epidemiology, circulating serotype and genotype among 61 dengue suspected cases from different hospitals of Nepal during the window period 2017–2018 between the two outbreaks of 2016 and 2019. E-gene sequences from PCR positive samples were subjected to phylogenetic analysis under time to most recent common ancestor tree using Markov Chain Monte Carlo (MCMC) and BEAST v2.5.1. Both evolution and genotypes were determined based on the phylogenetic tree. Serotyping by Real-time PCR and Nested PCR showed the co-circulation of all the 3 serotypes of dengue in the year 2017 and only DENV-2 in 2018. Genotype V for DENV-1 and Cosmopolitan Genotype IVa for DENV-2 were detected. The detected Genotype V of DENV-1 in Terai was found close to Indian genotype while Cosmopolitan IVa of DENV-2 found spreading to geographically safe hilly region (now gripped to 9 districts) was close to South-East Asia. The genetic drift of DENV-2 is probably due to climate change and rapid viral evolution which could be a representative model for high altitude shift of the infection. Further, the increased primary infection indicates dengue venturing to new populations. Platelets count together with Aspartate transaminase and Aalanine transaminase could serve as important clinical markers to support clinical diagnosis. The study will support future dengue virology and epidemiology in Nepal.
You have requested "on-the-fly" machine translation of selected content from our databases. This functionality is provided solely for your convenience and is in no way intended to replace human translation. Show full disclaimer
Neither ProQuest nor its licensors make any representations or warranties with respect to the translations. The translations are automatically generated "AS IS" and "AS AVAILABLE" and are not retained in our systems. PROQUEST AND ITS LICENSORS SPECIFICALLY DISCLAIM ANY AND ALL EXPRESS OR IMPLIED WARRANTIES, INCLUDING WITHOUT LIMITATION, ANY WARRANTIES FOR AVAILABILITY, ACCURACY, TIMELINESS, COMPLETENESS, NON-INFRINGMENT, MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE. Your use of the translations is subject to all use restrictions contained in your Electronic Products License Agreement and by using the translation functionality you agree to forgo any and all claims against ProQuest or its licensors for your use of the translation functionality and any output derived there from. Hide full disclaimer
Details
1 Tribhuvan University, Central Department of Biotechnology (CDBT), Kirtipur, Kathmandu, Nepal (GRID:grid.80817.36) (ISNI:0000 0001 2114 6728)
2 Clark Atlanta University, Department of Mathematical Sciences, Atlanta, USA (GRID:grid.254275.3) (ISNI:0000 0001 2224 3669)
3 Sukraraj Tropical and Infectious Disease Hospital (STIDH), Department of Tropical and Infectious Disease, Teku, Kathmandu, Nepal (GRID:grid.508276.e)
4 Tribhuvan University, Central Department of Biotechnology (CDBT), Kirtipur, Kathmandu, Nepal (GRID:grid.80817.36) (ISNI:0000 0001 2114 6728); Centre for Molecular Dynamics Nepal (CMDN), Department of Molecular Biology and Virology, Thapathali, Kathmandu, Nepal (GRID:grid.428196.0)
5 Balkumari College, Microbiology Department, Narayangarh, Chitwan, Nepal (GRID:grid.428196.0)
6 Trichandra Multiple Campus, Microbiology Department, Kathmandu, Nepal (GRID:grid.80817.36) (ISNI:0000 0001 2114 6728)
7 Kathmandu Research Institute for Biological Sciences (KRIBS), Department of Infection and Immunology, Lalitpur, Nepal (GRID:grid.80817.36)