Anti-Inflammatory and Cytotoxic Activities of Clerodane-Type Diterpenes

Abstract

The secondary metabolites of clerodane diterpenoids have been found in several plant species from various families and in other organisms. In this review, we included articles on clerodanes and neo-clerodanes with cytotoxic or anti-inflammatory activity from 2015 to February 2023. A search was conducted in the following databases: PubMed, Google Scholar and Science Direct, using the keywords clerodanes or neo-clerodanes with cytotoxicity or anti-inflammatory activity. In this work, we present studies on these diterpenes with anti-inflammatory effects from 18 species belonging to 7 families and those with cytotoxic activity from 25 species belonging to 9 families. These plants are mostly from the Lamiaceae, Salicaceae, Menispermaceae and Euphorbiaceae families. In summary, clerodane diterpenes have activity against different cell cancer lines. Specific antiproliferative mechanisms related to the wide range of clerodanes known today have been described, since many of these compounds have been identified, some of which we barely know their properties. It is very possible that there are even more compounds than those described today, in such a way that makes it an open field to discover. Furthermore, some diterpenes presented in this review have already-known therapeutic targets, and therefore, their potential adverse effects can be predicted in some way.

1. Introduction

Diterpenes are metabolites that come from isoprene units; these compounds can be classified according to their structure [1]. One type of diterpene is clerodanes, which are found in a wide range of plant species, especially those from the Labiatae, Euphorbiaceae and Verbenaceae families [2,3]; they have also been found in bacteria, fungi and marine sponges. This type of diterpene has been extensively studied due to many of them having biological activity [1,2,3,4]. For example, clerodin has anthelminthic activity [5]; salvinorin A is an agonist of κ-opioid receptor-serotonin-2A [6] with potential for use as a treatment in neuropsychiatric disorders [7]; tinosinenosides A–C show cytotoxicity effects against HeLa [8]; and columbin has anti-inflammatory and anticancer efficacy [4].

Clerodanes are secondary metabolites; when these compounds are obtained from plants, they are biosynthesized in the chloroplasts from geranylgeranyl pyrophosphate, producing a labdane-type precursor skeleton, which can be transformed to a halimane-type intermediate, and then converted to either cis- or trans- clerodanes [3] (Figure 1a).

Clerodanes are bicyclic diterpenoids with a fused ring of decalin structure (C₁–C₁₀) and a side chain of six carbons at C₉. They are classified according to the configuration at the ring fusion and the substituents in C₈ and C₉ into four types: trans-cis, trans-trans, cis-cis and cis-trans (Figure 1b). About 25% have a cis ring fusion, and 75% have 5:10 trans ring fusion [9].

In this review, we have included clerodanes and neo-clerodanes and their enantiomers ent-neo-clerodanes (Figure 2). Additionally, carbons 12 to 16 are usually oxidized to diene, furan, lactone or hydrofurofuran, which give structural characteristics to clerodane [10].

Cancer is a global health problem and is currently one of the main causes contributing to premature death worldwide [11]. At the present time, even with the great advances in medicine in our understanding and treatment of cancer with multimodal therapies including immunotherapy, gene-targeted therapy, chemotherapy, hormonal therapy and cancer vaccines [12] against specific cell targets, there are needs that have not been covered. These include more effective therapies, with fewer adverse effects, but also therapies at a more affordable cost. Thus, there is still a need to investigate more effective and less toxic compounds. Most of the chemotherapeutic drugs (nearly 65%) that are used in current cancer treatment regimens were originally isolated from natural products or their derivatives such as plants or microorganisms [13]. For instance, paclitaxel, a diterpene isolated from Taxus brevifolia (yew trees), classified as a taxane, is used in the therapy of various types of cancers [14]. Other examples include anthracyclines derived from Streptomyces strains, among them being doxorubicin, bleomycin and many others [13]. The cytotoxic activity of several clerodanes in different cancer cell lines has been described [1].

On the other hand, inflammation is an immune response to different stimuli, such as pathogens such as viruses and bacteria, traumas and chemical irritants [15]; that is to say, inflammation is a protective response of the body against harmful stimuli. Additionally, long-term inflammation could lead to several symptoms, such as pain, fatigue, insomnia, depression and gastrointestinal problems [16]. Chronic inflammation is associated with diseases such as cancer, diabetes and arthritis [17]. The inflammatory response leads to the production of pro-inflammatory mediators, such as cytokines, serotonin, leukotrienes and histamine [18]. These mediators promote vascular permeability, leukocyte migration, blood vessel dilatation and pain. The anti-inflammatory activity of terpenes, such as carvacrol, some carotenes and diterpenes, such as clerodanes, and triterpenes, has been studied [2,19].

In this review, 158 clerodanes and 70 neo-clerodanes (1, 56, 57, 71–73, 94–132, 141–158, 184–187, 196, 197 and 207–210) with cytotoxic and anti-inflammatory activities reported from 2015 to February of 2023 were included. A total of 56 articles were found; in Table 1, the plants, family, collection place and part of the plants from which the clerodanes and neo-clerodanes were isolated are shown.

Clerodanes and neo-clerodanes with cytotoxic activity are shown in Table 2, and their structures are shown in Figure 3, Figure 4, Figure 5, Figure 6, Figure 7, Figure 8, Figure 9, Figure 10, Figure 11, Figure 12 and Figure 13.

Clerodanes and neo-clerodanes’ anti-inflammatory activities are summarized in Table 3, and their structures are shown in Figure 14, Figure 15, Figure 16, Figure 17, Figure 18 and Figure 19.

2. Discussion

This review discusses research from the last 8 years on clerodane and neo-clerodane diterpenes that exhibit cytotoxic and anti-inflammatory activities. It presents studies on these diterpenes with anti-inflammatory effects from 18 species belonging to 7 families and those with cytotoxic activity from 25 species belong to 9 families. These plants mostly belong to the Lamiaceae, Salicaceae, Menispermaceae and Euphorbiaceae families. They include 228 clerodanes and neo-clerodanes, of which, 140 have cytotoxic activity, 88 have anti-inflammatory activity and crassifolin Q-U (49–53), compounds 74–77 and (-)-hardwickiic acid (91) have both activities. Compound 75 and 77 were alone in including acute toxicity, but they did not indicate LD₅₀.

2.1. Cytotoxic Activity

All clerodanes included in this review are oxygenated; 58% of them have at least one acetate group, 47% a hydroxyl group, 49% a ring of lactone and 22% a ring of furan as substituents. Additionally, it was found that three diterpenes isolated from Sheareria nana (125–127) have -OSO₃H.

We found that 82 compounds out of 140 were evaluated using the MTT assay, which is broadly used to measure the cytotoxic effects of drugs on cancer cell lines, and it is considered a quantitative cytotoxicity analysis; the assay is used more often because in itself, it is relatively straightforward and provides benefits due to the ease of its utility.

Compared to standard cancer therapies, in vitro studies have shown the cytotoxic and antiproliferative properties of different clerodane compounds. The mechanisms involved include growth inhibition, apoptosis, interference with DNA synthesis and driving DNA fragmentation in many cancer cell lines of mesenchymal, epithelial and hematopoietic origin [1,3].

Some clerodane compounds inhibit growth in cancer cell lines. Anacolosins A–F (3–8) and corymbulosins X and Y (9–10) isolated from Anacolosa clarkii exhibit cytotoxic properties in four paediatric cancer types [21]. Caseakurzin B (29) and caseakurzin J (34) from Casearia kurzii were investigated in a lung epithelial carcinoma cell line; the former arrested the cell cycle at the G2/M phase and the second at the S phase. Obtained from the same plant, corymbulosin M (25), caseamembrin B (26) and caseamembrin U (27) were also cytotoxic in three types of cancer cell lines. Of note, corymbulosin M (25) was the most potent of them and apparently even more active than etoposide, and it was shown that it affects the cell cycle at the G0/G1 stage [28]. Kurzipene D (38), also obtained from C. kurzii, has a potent antiproliferative effect compared to other kurzipenes and affects proliferation at the S stage. Further, one in vivo study used a xenograft tumor model in zebra fish embryos; this compound suppressed tumor proliferation and migration comparable to etoposide [26]. Crassifolins Q-U (49–53) from Croton crassifolius inhibited angiogenesis in HUVECs, and crassifolin U (53) had the strongest activity in this model [32]. Notably, the antitumor properties of casearins have been shown using in vivo and ex vivo methods [30]. Epoxy clerodane diterpene (139) isolated from Tinospora cordifolia had cytotoxic activity, inhibiting MCF7 growth by regulating the expression of the functional genes Rb1 and Mdm2 [55].

Several specific antiproliferative mechanisms related to the wide range of clerodanes known today have been described, since many of these compounds have been identified, some which we barely know their properties. It is very possible that there are even more compounds than those described today, in such a way that makes it an open field to discover. However, it is important to mention that clinical studies are required to demonstrate their efficacy in the therapy of the current cancer pandemic, and demonstrating their safety is also of great importance.

2.2. Anti-Inflammatory Activity

A total of 45% of the clerodanes with anti-inflammatory activity have at least one hydroxyl, 69% compounds contain a ring of lactones, 50% a ring of furans and 26% an acetate group as substituents.

We found that 63 compounds reported to have anti-inflammatory activity were evaluated for nitric oxide inhibition with the Griess assay on RAW264.7 macrophages or BV-2-cell-stimulated-LPS, and the clerodanes 157, 158, 185, 186 and 207 showed the best activity in this test with IC₅₀ values of less than 2 µM. In this review, we found that in vivo studies have only been performed for hautriwaic acid (196) and nepetolide (204).

The anti-inflammatory activity of clerodane diterpenoids mediated by different mechanisms has been demonstrated in in vitro and in vivo animal models. Compounds 154, 155, 157 and 158 from Callicarpa arborea showed potent inhibitory effects against the NLRP3 inflammasome by inhibiting Casp-1 activation and IL-1β in reticulum cell sarcoma cells [59].

Clerodane 74–77 and 206 from extracts of Polyalthia longifolia seeds inhibit inflammation, blocking the synthesis of prostaglandins and leukotrienes through highly selective binding to cyclooxygenases (COX) 1 and 2 and 5-lipooxygenase (5-LOX), respectively, compared to the nonsteroidal anti-inflammatory drugs diclofenac and indomethacin [71]. In 2008, clerodane 206 was associated with the suppression of neutrophil respiratory burst and degranulation, and it is thought that it is mediated at least in part by the inhibition of calcium mobilization, AKT (protein kinase B) and p38 mitogen-activated protein kinase pathways [77]. Hautriwaic acid (196) from Dodonaea viscosa leaves, used for rheumatism, exhibited anti-inflammatory activity in a mouse ear edema model [66]. Clerodane compounds 164–175 from Callicarpa hypoleucophylla suppress superoxide anion generation and elastase release, inhibiting the function of human neutrophils [61]. Trans-crotonin inhibits dextran- and histamine-induced oedema [2].

Compounds derived from the Scutellaria genus have strong interactions with inducible nitric oxide synthase, and because of that, they inhibit nitric oxide production [72]. Five clerodane diterpenoids from Croton crassifolius roots, named crassifolins Q–U (49–53), reduced the levels of IL-6 and TNF-α in lipopolysaccharide-stimulated RAW 264.7 cells [32]. Compounds 211–213 from Tinospora crispa diminish the production of pro-inflammatory mediators (IL-1β, IL-6, TNF-α, iNOs, CCL12 and COX-2) [74].

3. Conclusions

In summary, clerodane diterpenes have activity against different cell cancer lines. Furthermore, some of the diterpenes presented in this review have already-known therapeutic targets, and therefore, their potential adverse effects can be predicted in some way, but the discovery of new compounds and new mechanisms remains to be seen. Anyway, the study of possible new therapies for inflammation continues to be important in order to expand the options for the treatment of inflammatory diseases that afflict the world.

More than 50% of clerodanes included in this review with cytotoxic activity contain acetate groups; on the other hand, 69% of the compounds with anti-inflammatory effects have a ring of lactone.

Author Contributions

Conceptualization, J.P.-R. and S.P.-G.; methodology, R.M.M.-C., L.H.-V., A.M., L.S.-P., S.P.-G. and J.P.-R.; validation R.M.M.-C., L.H.-V., A.M., L.S.-P., S.P.-G. and J.P.-R.; formal analysis, R.M.M.-C., L.H.-V., A.M., L.S.-P., S.P.-G. and J.P.-R.; data curation, R.M.M.-C., L.H.-V., A.M., L.S.-P., S.P.-G. and J.P.-R.; writing—original draft preparation, S.P.-G. and A.M.; writing—review and editing, R.M.M.-C., L.H.-V., A.M., L.S.-P., S.P.-G. and J.P.-R. All authors have read and agreed to the published version of the manuscript.

Institutional Review Board Statement

Not applicable.

Informed Consent Statement

Not applicable.

Data Availability Statement

Not applicable.

Conflicts of Interest

The authors declare no conflict of interest.

Footnotes

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Figures and Tables

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Figure 1. (a) Biosynthesis of clerodanes and (b) general structure of clerodanes.

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Figure 2. Absolute clerodane configuration.

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Figure 3. Isolated compound of Ajuga decumbens and Anacolosa clarkii.

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Figure 4. Isolated compounds of different species of Casearia.

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Figure 5. Isolated compounds of different species of Casearia (continued).

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Figure 6. Isolated compounds of different species of Croton.

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Figure 7. Isolated compounds of Gottschelia schizopleura and Laetia corymbulosa.

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Figure 8. Isolated compounds of Linaria japonica and Polyalthia longifolia.

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Figure 9. Isolated compounds of different species of Salvia.

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Figure 10. Isolated compounds of Scutellaria barbata (continued).

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Figure 11. Isolated compounds of Scutellaria barbata.

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Figure 12. Isolated compounds of Scutellaria strigillosa.

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Figure 13. Isolated compounds of Sheareria nana, Tinospora capillipes, Tinospora cordifolia and Tinospora sagittata.

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Figure 14. Compounds of Ajuga pantantha and Callicarpa arborea with anti-inflammatory activity.

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Figure 15. Compounds of Callicarpa cathayana and Callicarpa hypoleucophylla with anti-inflammatory activity.

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Figure 16. Compounds of different species of Croton with anti-inflammatory activity.

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Figure 17. Compounds of different species of Croton with anti-inflammatory activity (continued).

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Figure 18. Compounds of Dodonaea viscosa, Dysoxylum lukii, Jamesoniella autumnalis, Monon membranifolium, Nepeta suavis, Polyalthia longifolia and Scutellaria barbata with anti-inflammatory activity.

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Figure 19. Compounds of different species of Teucrium fructicans, Tinospora crispa and Tinospora sagittata with anti-inflammatory activity.

Table 1

Part of plant, family and collection place of plants that contained clerodanes or neo-cleordanes.

Plant	Family	Part of Plant	Collection Place
Ajuga decumbens [20]	Lamiaceae	Aerial parts	Pingtan island of Fujian Province.
Anacolosa clarkia [21]	Olacaceae	Fruit, leaves and twigs of the plant	Bana Forest Preserve in Danang. NCI Natural Products Repository.
Casearia corymbosa [22]	Salicaceae	Stem bark	Othón P. Blanco, Quintana Roo, Mexico.
Casearia graveolens [23]	Salicaceae	Twigs	Chiang Rai Province, northern Thailand.
Casearia grewiifolia [24,25]	Salicaceae	Fresh fruits	Khon Kaen University campus.
		Leaves	Phu Loc–Thua Thien Hue, Vietnam.
Casearia kurzii [26,27,28,29]	Salicaceae	Fruit, leaves and twigs	Bana Forest Preserve in Danang, Vietnam.
		Twigs and leaves	Xishuangbanna County, Yunn an Province, P. R. China.
Casearia sylvestris [30]	Salicaceae	Leaves	Parque Estadual Carlos Botelho (São Miguel Arcanjo, São Paulo State.)
Croton caudatus [31]	Euphorbiaceae	Leaves and twigs	Xishuangbanna Prefecture, Yunnan Province, P. R. China.
Croton crassifolius [32,33,34]	Euphorbiaceae	Roots	Yulin City, Guangxi Province, China.
			Southeast China, Thailand, Vietnam, and Laos.
			Fujian Province, People’s Republic of China.
Croton echioides [35]	Euphorbiaceae	Stems	Brazil
Croton oligandrus [36]	Euphorbiaceae	Bark	Mount Eloundem, Central Region, Cameroon.
Gottschelia schizopleura [37]	Cephaloziellaceae	Aerial parts	Mount Alab, Sabah, North Borneo, Malaysia.
Laetia corymbulosa [38]	Salicaceae	Bark	The plant was provided by NCI/NIH (Frederick, MD, U.S.).
Linaria japonica [39]	Plantaginaceae	Whole plants	Hiroshima, Japan.
Polyalthia longifolia [40]	Annonaceae	Seeds	Tirupati, India.
Polyalthia laui [41]	Annonaceae	Roots	Hainan Province, China.
Salvia amarissima [42,43,44]	Lamiaceae	Leaves and flowers	Teotihuacan, State of Mexico.
		Aerial portions	Teotihuacan Valley
Salvia involucrata [45]	Lamiaceae	Aerial parts	Municipality of Xilitla, State of San Luis Potosí, Mexico.
Salvia leucantha [46]	Lamiaceae	Aerial parts	Yunnan Province, China.
Scutellaria barbata [47,48,49,50]	Lamiaceae	Whole plant	Linyi district, Shandong Province, China.
		Aerial parts	Purchased in a drugstore of Liaoning Guodayizhi Pharmaceutical Co., Ltd. China.
		Aerial parts	Purchased from Bozhou Herbal Market in Anhui Province, China
Scutellaria strigillosa [51,52]	Lamiaceae	Whole plants	Yantai district, Shandong Province, China.
		Whole plants	Hebei, Shandong, Zhejiang and Jilin Provinces, China
Sheareria nana [53]	Asteraceae	Whole herb	Jishou, Hunan Province, China.
Tinospora capillipes [54]	Menispermaceae	Whole herb	Xishuangbanna County, Yunnan Province, China.
Tinospora cordifolia [55]	Menispermaceae	Stems	India
Tinospora sagittata [56]	Menispermaceae	Roots	Anguo Medicine market in Hebei Province, China.
Ajuga pantantha [57,58]	Lamiaceae	Aerial parts	Yunnan Province, China.
		Aerial parts	Purchased from Anhui Province, China.
Callicarpa arborea [59]	Lamiaceae	Twigs	Xishuangbanna and Yuanyang Prefectures.
Callicarpa cathayana [60]	Lamiaceae	Dried aerial parts	Bozhou Herbal Market in Anhui Province, China.
Callicarpa hypoleucophylla [61]	Lamiaceae	Leaves and twigs	Kaohsiung city, Taiwan.
Croton crassifolius [32,62]	Euphorbiaceae	Roots	Guangxi Province, China.
Croton floribundus [63]	Euphorbiaceae	Roots	Provided by the company Mudas Nativas e Exóticas.LTDA of CNPJ, Araraquara Brazil.
Croton laui [64]	Euphorbiaceae	Leaves	Hainan Province, China.
Croton poomae [65]	Euphorbiaceae	Leaves and stems	Bung Kan Province, Thailand.
Dodonaea viscosa [66]	Sapindaceae	Leaves	Sierra de Huautla, Morelos State, Mexico.
Dysoxylum lukii. [67]	Meliaceae	Twigs and leaves	Xishuangbanna County, Yunnan Province, China.
Jamesoniella autumnalis [68]	Adelanthaceae	Whole plant	Wangtiane park, Changbaishan City, Jilin Province, China.
Monoon membranifolium [69]	Annonaceae	Twig extract	Thailand and Peninsula Malaysia.
Nepeta suavis [70]	Lamiaceae	Roots	Found in central and southern Europe, North Africa and southern Asia.
Polyalthia longifolia [71]	Annonaceae	Seeds	Seshachalam hills,Tirupati, India.
Scutellaria barbata [72]	Lamiaceae	Aerial parts	Baise city, Guangxi Province, China.
Teucrium fructicans [73]	Lamiaceae	Aerial parts	Jiansu Province, China.
Tinospora crispa [74,75]	Menispermaceae	Stems	Mengla County, Yunnan Province, China.
		Vines and leaves	Longzhou County, Guangxi Province, China.
Tinospora sagittata [76]	Menispermaceae	Tuberous roots	Shiyan city of Hubei Province, China.

Table 2

Clerodane diterpenes with cytotoxic activity.

Plant Source	Compound Name	Methods	Results	References
Ajuga decumbens	Compound 1	CCK8 methodA549HeLa	IC50 µM71.471.6	[20]
	Ajugamarin A1 (2)	A549HeLa	76.75.39 × 10−7
Anacolosa clarkii	Anacolosin A (3)	SRB assayA-673SJCRH30D283Hep293TT	TGI μM1.100.520.701.00	[21]
	Anacolosin B (4)	A-673SJCRH30D283Hep293TT	1.000.500.600.90
	Anacolosin C (5)	A-673SJCRH30D283Hep293TT	1.100.670.661.00
	Anacolosin D (6)	A-673SJCRH30D283Hep293TT	1.200.730.660.80
	Anacolosin E (7)	A-673SJCRH30 D283Hep293TT	3.101.902.001.80
	Anacolosin F (8)	A-673SJCRH30D283Hep293TT	4.102.302.303.20
	Corymbulosin X (9)	A-673SJCRH30D283Hep293TT	0.700.340.360.22
	Corymbulosin Y (10)	A-673SJCRH30D283Hep293TT	1.000.440.700.28
	Compound 11	A-673SJCRH30D283Hep293TT	1.700.801.100.60
	Caseamembrin S (12)	A-673SJCRH30 D283Hep293TT	0.900.360.500.30
Casearia corymbosa	Casearborin c (13)	SRB assayHeLaSiHaVero	CC50µM (SI)13.4477.3650.26	[22]
Casearia graveolens	Caseariagraveolin (14)	REMA assayKBMCF-7	IC50 μM2.486.63	[23]
Casearia grewiifolia	Caseargrewiin M (15)	MTT assayBT474Chago-K1Hep-G2KATO-IIISW620	IC50 µg/mL6.306.104.645.505.50	[24,25]
	Caseargrewiin G (16)	BT474Chago-K1Hep-G2KATO-IIISW620	5.676.100.905.463.85
	Caseagrewiifolin B (17)	WST-1 assayKBHep-G2	IC50 μM6.27.0
	Caseanigrescen D (18)	KBHep-G2LU-1MCF-7NIH-3T3	0.50.30.90.80.3
Casearia kurzii	Kurziterpene A (19)	MTT assayA549,HeLaHepG2	IC50 μM40.8>60>60	[26,27,28,29]
	Kurziterpene B (20)	A549HeLaHep-G2	19.712.149.3
	Kurziterpene C (21)	A549,HeLaHep-G2	>6049.4>60
	Kurziterpene D (22)	A549,HeLaHep-G2	18.39.0>60
	Kurziterpene E (23)	A549,HeLaHep-G2	10.25.310.7
		Analysis via flow cytometry	Apoptosis of HeLa
	(2R,5S,6S,8R,9R,10S,18S,19S)-2,19-diacetoyloxy-6,18-dimethoxy-18,19-epoxycleroda-3,13(16),14-triene (24)	MTT assayA549HeLaHep-G2	IC50 μM>6017.9>60
	Corymbulosin M (25)	A549HeLaHep-G2	5.54.19.3
		Analysis via flow cytometry	Apoptosis of HeLa
	Caseamembrin B (26)	MTT assayA549HeLaHep-G2	IC50 μM36.118.8>60
	Caseamembrin U (27)	A549HeLaHep-G2	33.215.6>60
	Caseakurzin A (28)	QIR assayA549	IC50 μM10.8
	Caseakurzin B (29)	QIR assayA549	IC50 μM4.4
		Cell apoptosis assay	Apoptosis of A549
	Caseakurzin C (30)	QIR assayA549	IC50 μM30.3
	Caseakurzin D (31)		27.8
	Caseakurzin E (32)		32.7
	Caseakurzin F (33)		26.8
	Caseakurzin J (34)	QIR assayA549	IC50 μM4.6
		Cell apoptosis assay	Apoptosis of A549
	Kurzipene A (35)	MTT assayHep-G2A549HeLa K562	IC50 μM>60>60>60>60
	Kurzipene B (36)	Hep-G2A549HeLaK562	>6032.654.6>60
	Kurzipene C (37)	Hep-G2A549HeLa K562	>60>60>60>60
	Kurzipene D (38)	Hep-G2A549HeLaK562	9.710.912.47.2
		Flow cytometry	Apoptosis of Hep-G2
		Anti-tumor assay using zebrafish model	It blocked tumor cell invasion and metastasis
	Kurzipene E (39)	Hep-G2A549HeLaK562	>60>60>60>60
	Kurzipene F (40)	Hep-G2A549HeLaK562	>60>6033.1>60
	Corymbulosin V (41)	Hep-G2A549HeLaK562	16.811.214.210.3
	Corymbulosin M (25)	Hep-G2A549HeLaK562	20.618.417.516.5
Casearia sylvestris	Casearin X (42)	Induced sarcoma tumor25 mg/kg/day	Tumor inhibition %90.0	[30]
Croton caudatus	Crocleropene A (43)	MTT assayMCF-7	IC50 μM35.8	[31]
	Crocleropene B (44)	MCF-7	40.2
Croton crassifolius	Crassifolius A (45)	Morphology	Induced apoptosis	[32,33,34]
		Western blot	Caspase activation
		MTT assayHep3BHep-G2	IC50 µM17.9142.04
	Crassifolin C (46)	Hep-G2	51.63
	Compound 47	Hep-G2	45.22
	Crassifolin B (48)	CT26.WT	96.6
	Crassifolin Q (49)	HUVEC assay	Compounds 49–51 and 53 inhibited angiogenesis
	Crassifolin R (50)
	Crassifolin S (51)
	Crassifolin T (52)	HUVEC assay	Anti-angiogenesis effect
	Crassifolin U (53)	HUVEC assayJunction densitiesVessel areasVessel lengths	IC50 μM7.2048.278.62
Croton echioides	CEH-1 (54)	MTT assayHTC	Compound 54 diminished 67% cell viability and 55 < 76%.	[35]
	CEH-4 (55)
Croton oligandrus	Megalocarpoidolide D (56)	MTT assayA549MCF-7	IC50 µM 63.8136.2.	[36]
	12-epi-megalocarpodolide D (57)	A549MCF-7	138.6171.3
Gottschelia schizopleura	Schizopleurolide A (58)	MTT assayHL-60B16-F10	IC50 µM38.4747.25	[37]
	Schizopleurolide B (59)	HL-60B16-F10	36.1344.33
Laetia corymbulosa	Corymbulosin I (60)	Flow cytometry	Compounds 60, 61, 12 and 11 induced apoptosis in MDA-MB-231	[38]
		SRB assayA549MDA-MB-231MCF-7KBKB-VIN	IC50 µM 0.660.480.680.560.98
	Corymbulosin K (61)	A549MDA-MB-231MCF-7KBKB-VIN	0.470.490.500.450.49
	Corymbulosin L (62)	A549MDA-MB-231MCF-7KBKB-VIN	4.604.954.945.194.92
	Corymbulosin N (63)	A549MDA-MB-231MCF-7KBKB-VIN	5.044.905.825.235.19
	Corymbulosin O (64)	A549MDA-MB-231MCF-7KBKB-VIN	4.753.314.654.254.76
	Corymbulosin P (65)	A549MDA-MB-231MCF-7KBKB-VIN	5.984.936.395.165.03
	Corymbulosin Q (66)	A549MDA-MB-231MCF-7KBKB-VIN	40.220.531.719.839.2
	Corymbulosin S (67)	A549MDA-MB-231 MCF-7KBKB-VIN	>4022.926.225.126.6
	Corymbulosin T (68)	A549MDA-MB-231 MCF-7KBKB-VIN	2.290.490.690.560.61
	Corymbulosin V (41)	A549MDA-MB-231 MCF-7KBKB-VIN	4.764.735.194.744.88
	Caseamembrin S (12)	A549MDA-MB-231 MCF-7KBKB-VIN	0.580.450.660.530.90
	Caseamembrin E (69)	A549MDA-MB-231 MCF-7KBKB-VIN	0.530.400.550.430.51
	Corymbulosin A (70)	A549MDA-MB-231 MCF-7KBKB-VIN	0.450.430.440.420.45
	Compound 11	A549MDA-MB-231 MCF-7KBKB-VIN	4.150.540.890.734.07
Linaria japonica	Linarenone C (71)	MTT assayA549	IC50 µM51.2	[39]
	Linarenone E (72)		86.5
	Linarienone (73)		79.0
Polyalthia longifolia	16-hydroxy-cleroda-4(18),13-dien-16,15-olide (74)	Evaluation of morphometric liver and biochemical parameters in (NDEA+PB)-induced HCC rats	Compound 75 and 77 restored the parameters’ biochemical and liver morphology	[40]
		MTT assayHep-G2	IC50 µg/mL34.33
	3α,16α-dihydroxy-cleroda-4(18),13(14)Z-dien-15,16-olide (75)	Hep-G2HuH-7	14.3447.32
	16α-hydroxy-cleroda-3,13(14)Z-dien-15,16-olide (76)	Hep-G2	29.21
	3β-16a-dihydroxy-cleroda-4(18),13(14)Z-dien-15,16-olide (77)	Hep-G2HuH-7	24.9148.57
Polyalthia laui	Polylauiester A (78)	MTT assayHeLaMCF-7A549	IC50 μM34.8433.2135.65	[41]
	(4→2)-abeo-2,13-diformyl-cleroda-2,12E-dien-14-oic acid (79)	HeLaMCF-7A549	39.3137.3537.82
	Polylauiamide B (80)	HeLaMCF-7A549	28.0929.1629.25
	Polylauiamide C (81)	HeLaMCF-7 A549	25.0130.3028.65
	Polylauiamide D (82)	HeLaMCF-7A549	26.7327.0328.88
Salvia amarissima	Teotihuacanin (83)	SRB assayMDA-MB-231HeLaHCT-15HCT-116MCF-7	IC50 μM12.313.712.910.9>20	[42,43,44]
	Amarissinin A (84)	MCF-7MCF-7/Vin+MDA-MB-231HeLa	18.20.2719.314.0
	Amarissinin B (85)	SRB assay	83, 84, 85, 86 and 87 exhibited MDR modulatory effects in mammalian cancer cells
	Amarissinin C (86)
	Amarisolide F (87)	SRB assayMCF-7HeLaHCT-15HCT-116MDA-MB-231	IC50 μM42.1>42>42>42>42
Salvia involucrata	Involucratin A (88)	U251PC-3K562SKLU-1	49.614.724.812.6	[45]
	Involucratin B (89)	U251PC-3K562HCT-15MCF-7SKLU-1COS-7	5.123.534.711.80.536.721.6
	Involucratin C (90)	PC-3K562HCT-15SKLU-1COS-7	11.019.49.716.811.9
	(-)-Hardwickiic acid (91)	U251PC-3K562HCT-15MCF-7SKLU-1COS-7	22.41.845.510.41.411.519.8
	7α-hydroxybacchotricuneatin A (92)	U251PC-3K562HCT-15SKLU-1COS-7	3.812.820.213.333.014.2
	Kingidiol (93)	SRB assayU251PC-3K562HCT-15MCF-7SKLU-1COS-7	IC50 μM22.413.051.615.50.822.919.7
Salvia leucantha	Salvileucantholide (94)	MTT assayHCT-116BT474HepG2Hsp90	IC50 µM32.6125.0237.356.78	[46]
Scutellaria barbata	Scubatine A (95)	MTT assayHL-60A549	IC50 µM>20>20	[47,48,49,50]
	Scubatine B (96)	HL-60A549	>20>20
	Scubatine C (97)	HL-60A549	>20>20
	Scubatine D (98)	HL-60A549	>20>20
	Scubatine E (99)	HL-60A549	>20>20
	Scubatine F (100)	HL-60A549	15.310.4
	Scutebata E (101)	MTT assayHL-60A549LoVo	IC50 µM>20>2061.23
	Scutolide K (102)	HL-60A549	>20>20
	Scutebata X (103)	SGC-7901MCF-7A549	>4037.2>40
	Scutebata Y (104)	SGC-7901MCF-7A549	>40>40>40
	Scutebata Z (105)	SGC-7901MCF-7A549	>40>40>40
	Scutebata A1 (106)	SGC-7901MCF-7A549	>40>4035.5
	Scutebata B1 (107)	SGC-7901MCF-7A549	>40>40>40
	Scutebata C1 (108)	SGC-7901MCF-7A549	17.929.935.7
	Barbatin H. (109)	LoVoMCF-7SMMC-7721HCT-116	32.4449.8648.7544.24
	Scuterbarbatine F (110)	LoVoMCF-7 SMMC-7721HCT-116	23.3249.1958.1278.83
	6-O-nicotinoylscutebarbatine G (111)	LoVo SMMC-7721HCT-116	29.4465.5154.44
	Scutebata G (112)	LoVo MCF-7 SMMC-7721HCT-116	22.5631.3332.4928.29
	Scutebata D (113)	LoVoMCF-7 SMMC-7721HCT-116	20.7531.4229.2462.66
	Barbatin C (114)	LoVoMCF-7 SMMC-7721 HCT-116	37.9928.0672.6932.94
	Scutebarbatine A (115)	LoVo	67.77
	Scutebarbatine G (116)	LoVoSMMC-7721 HCT-116	56.46 70.1644.25
	6,7-di-O-acetoxybarbatin A (117)	LoVoMCF-7 SMMC-7721 HCT-116	60.3337.3177.9332.28
	Scutebarbatine X (118)	LoVoMCF-7 SMMC-7721 HCT-116	43.21 74.83 46.14 62.11
	Barbatin F (119)	LoVo HCT-116	56.46 44.25
	Barbatin G (120)	LoVoSMMC-7721 MCF-7 HCT-116	60.3337.31 77.93 32.28
	Scutebata A (121)	LoVoSMMC-7721 MCF-7 HCT-116HL-60A549	4.577.68 5.31 6.23>20>20
	Scutebata B (122)	LoVoSMMC-7721 MCF-7 HCT-116	10.7318.96 10.27 28.48
	Scutebata C (123)	LoVoSMMC-7721 MCF-7	47.15 33.18 38.79
	Scutebata P (124)	LoVoSMMC-7721 MCF-7 HCT-116HL-60A549HCT-116	15.1742.63 32.49 23.975.621.723.97
Scutellaria strigillosa	Scutestrigillosin A (125)	REMA assayP-388HONE-1,HT-29MCF-7	IC50 μM5.83.54.75.7	[51,52]
	Scutestrigillosin B (126)	P-388HONE-1HT-29MCF-7	5.24.24.16.0
	Scutestrigillosin C (127)	P-388HONE-1,HT-29MCF-7	7.13.96.47.7
	Scutestrigillosin D (128)	P388HONE 1HT-29MCF-7	5.63.44.75.2
	Scutestrigillosin E (129)	P388HONE 1HT-29MCF-7	8.97.38.17.4
Sheareria nana	Sheareria A (130)	CCK8 assayHeLaPANC-1A549	IC50 µM11.67.19.3	[53]
	Sheareria B (131)	HeLaPANC-1A549	9.45.66.8
	Sheareria C (132)	HeLaPANC-1A549	17.29.812.5
Tinospora cordifolia	Tinocapillin A (133)	MTT assayA549HepG2HeLaOS-RC-2	IC50 µM14.09.99.710.6	[54]
	Tinocapillin B (134)	A549HepG2HeLaOS-RC-2	9.610.112.019.1
	Tinocapillin C (135)	A549HeLa	53.267.5
	Tinocallone A (136)	A549HepG2HeLa	67.868.479.3
	Tinocallone C (137)	A549HepG2HeLaOS-RC-2	16.313.817.512.8
	Columbin (138)	A549HeLa	77.358.4
Tinospora capillipes	ECD (epoxy clerodane diterpene) (139)	MTT assayV79MCF-7Vero	IC50 µM52.73.245.8	[55]
		qPCR analysis	Inhibited MCF-7 grow by regulation the expression of genes such Cdkn2A, Rb1, Mdm2 y p53
Tinospora sagittata	Tinosporin A (140)	MTT assayHL-60 MCF-7	IC50 µM18.6323.58	[56]

Compound 1 (1S,4aS,5R,6S,8R,8aS)-8-acetoxy-5-((R)-2-acetoxy-2-(5-oxo-2,5-dihydrofuran-3-yl)ethyl)-2-hydroxy-5,6-dimethyloctahydro-8aH-spiro[naphthalene-1,2′-oxiran]-8a-yl)methyl (E)-2-methylbut-2-enoate; Compound 11 (2R,5S,6S,8R,9R,10S,18R,19S)-18,19-di-O-acetyl-18,19-epoxy-6-hydroxy-2-(2′-methylbutanoyloxy)cleroda-3,13-(16),14triene; Compound 47 6-[2-(furan-3-yl)-2-oxoethyl]-1,5,6-trimethyl-10-oxatricyclo[7.2.1.02,7] dodec-2(7)-en-11-one. 3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide (MTT); sulforhodamine B (SRB); N-nitrosodiethylamine and phenobarbital sodium (NDEA+PB); cell counting kit 8 assay (CCK8); resazurin microplate assay (REMA); protein 90 kDa of family of chaperones (Hsp90); concentration cytotoxic at 50% (CC₅₀); quinone reductase assay (QIR); selective index (SI); total growth inhibitory (TGI); breast cancer (MCF-7); breast cancer resistant at vinblastine (MCF-7/Vin); breast ductal carcinoma (BT474); cervix adenocarcinoma (HeLa); cervix squamous carcinoma (SiHa); colon adenocarcinoma (SW620, HCT-15, HCT-116 and HT-29) colon cancer (LoVo); chronic myeloid leukemia (K562); epidermoid carcinoma of the nasopharynx (KB); Ewing sarcoma (A-673); gastric carcinoma (KATO-III, SGC-7901); glioblastoma (U251); hepatocarcinoma (Hep293TT, Hep3B, Hep-G2, SMMC-7721, HCC, HuH-7); human umbilical vein endothelial cells (HUVEC); liver tumor cells of Rattus norvegicus (HTC); lymphoma cells (P388); lung adenocarcinoma (LU-1, SKLU-1, A549); medulloblastoma (D283); mouse colon adenocarcinoma (CT26.WT); mouse embryonic fibroblast cell line (NIH-3T3); musculus skin melanoma (B16-F10); normal green monkey kidney cell line (Vero); normal monkey kidney (COS-7); normal prostate epithelium (PNT2); promyelocytic leukemia (HL-60); prostate cancer (PC-3); P-gp-overexpressing MDR subline of KB (KB-VIN); pancreatic carcinoma (PANC-1); renal carcinoma (OS-RC-2); rhabdomyosarcoma (SJCRH30); triple-negative breast cancer (MDA-MB-231); two epithelial tumor cell lines (HNE-1 and HONE-1; undifferentiated lung carcinoma (Chago-K1).

Table 3

Clerodane diterpenes with anti-inflammatory activity.

Plant Source	Compound Name	Methods	Results	References
Ajuga pantantha	Ajugapantin C (141)	Western Blot Analysis	Compounds 141, 142 and 146 downregulated iNOS and COX-2 protein levels	[57,58]
		Docking Analysis	Compounds 141, 142 and 146 have strong interactions with the iNOS and COX-2 proteins
		Griess assayBV-2 cells stimulated LPS	IC50 µM20.2
	Ajugapantin E (142)	Griess assayBV-2 cells stimulated LPS	IC50 µM45.5.
	Ajugapantin F (143)		34.0
	Ajugapantin G (144)		27.0
	Ajugapantin H (145)		45.0
	Ajugapantin I (146)		25.8
	Pantanpene α (147)	Griess assayBV-2 cells stimulated LPS	IC50 μM 65.7
	Pantanpene B (148)		37.7
	Pantanpene C (149)		61.7
	Pantanpene d (150)		>50% inhibition at 30 μM
	Pantanpene E (151)	Griess assayBV-2 cells stimulated LPS	IC50 μM 21.7
		Anti-inflammatory assay in zebrafish model	The anti-inflammatory effect was confirmed
		Docking Analysis	Compounds 148 and 151 have strong interactions with the iNOS and COX-2 proteins
Callicarpa arborea	Callicarpin A (152)	NLRP3 Inflammasome activation assayJ774A.1 cells were primed with LPS	IC50 μM16.6	[59]
	Callicarpin B (153)		4.0
	Callicarpin C (154)		25.4
	(16S)-Tris-O-Acetylcallicarpin C (155)		5.3
	Callicarpin E (156)		24.7
	Callicarpin F (157)		1.5
	Callicarpin G (158)	NLRP3 Inflammasome activation assayJ774A.1 cells were primed with LPS	IC50 μM1.4
		Pyroptosis fluorescence microscopy	The compound 153 inhibited pyroptosis and blocked NLRP3 inflammasome activation by hampering Casp-1 cleavage and IL-1β secretion
Callicarpa cathayana	Cathayanalactone A (159)	Griess assayRAW264.7 macrophages stimulated LPS	IC50 µM22.92	[60]
	Cathayanalactone B (160)		13.25
	Cathayanalactone C (161)	Griess assayRAW264.7 macrophages stimulated LPS	IC50 µM82.82
	15-methoxypatagonic acid (162)		35.35
	16-hydroxycleroda-3, 13-dien-16, 15-olide-18-oic acid (163)	Griess assayRAW264.7 macrophages stimulated LPS	IC50 µM17.49
		ELISA assayQuantification of TNF-α, IL-6 and IL-1β	Compounds 161–163 inhibited IL-1β, IL-6 and TNF-α
Callicarpa hypoleucophylla	Callihypolin A (164)	Inhibitory activities in- superoxide anion generation and - elastase release in formyl-methionyl-leucyl-phenylalanine (fMLF)/cytochalasin (CB)-induced human neutrophils	% of inhibition 20.288.26	[61]
	Callihypolin B (165)		32.1917.55
	Compound 166		31.1912.15
	Patagonic acid (167)		32.8813.57
	Limbatolide F (168)		23.657.33
	Limbatolide A (169)		8.4410.50
	Compound 170		7.939.30
	Clerodermic acid (171)		15.2311.80
	Visclerodol acid (172)		18.8016.30
Croton crassifolius	Crassifolin Q (49)	ELISA assayIL-6TNF-α	% of production72.2389.38	[32,62]
	Crassifolin R (50)		77.8877.73
	Crassifolin S (51)		73.3679.23
	Crassifolin T (52)		35.4854.14
	Crassifolin U (53)		32.7812.53
	Compound 173	Griess assayRAW264.7 macrophages stimulated LPS	IC50 μM25.8
	Compound 174		173 at 178 < 50% inhibition at 50 µM
	C-6 epimer of crotoeuricin C (175)
	Crotocaudin (176)
	Teucvin (177)
	Crassifolin F (178)
Croton floribundus	Croflorin A (179)	Griess assayRAW264.7 macrophages stimulated LPS	IC50 μM28.52	[63]
	Croflorin B (180)		40.26
	Croflorin C (181)		25.47
	Croflorin D (182)		35.78
	3α-hydroxy-5,10-didehydrochiliolide (183)		40.58
Croton laui	3S-acetoxyl-mollotucin D dilactone ester (184)	Griess assayRAW264.7 macrophages stimulated LPS	IC50 µMweak activity	[64]
	6S-crotoeurin C (185)		1.2
	Crotoeurin C (186)		1.6
	Mollotucin D dilactone ester (187)		weak activity
	Crassifolin F compound 178		weak activity
Croton poomae	Crotonolide K (188)	Griess assayRAW264.7 macrophages stimulated LPS	IC50 µM46.43	[65]
	Furocrotinsulolide A acetate (189)		31.99
	Furocrotinsulolide A (190)		81.97
	Compound 191		86.98
	Compound 192		48.85
	Crotonolide E (193)		74.78
	Crotonolide F (194)		42.04
	Compound 195		32.19
Dodonaea viscosa	Hautriwaic acid (196)	Arthritis in mice induced bycaolin/carrageenanDoses mg/kg51020	% inflammation of edema after 15 days 272013	[66]
		ELISA assayQuantification of IL-10, TNF-α, IL-6 and IL-1β	Compound 196 diminished TNF-α, IL-6 and IL-1β and increased IL-10
Dysoxylum lukii.	neoclerod-13Z-ene-3α, 4β, 15-triol (197)	Griess assayRAW264.7 macrophages stimulated LPS	IC50 µM.25.5	[67]
Jamesoniella autumnalis	Jamesoniellide Q (198)	Griess assayRAW264.7 macrophages stimulated LPS	IC50 µM 45.10	[68]
	Jamesoniellide R (199)		82.98
Monoon membranifolium	2β-Methoxyhardwickiic acid (200)	Griess assayRAW264.7 macrophages stimulated LPS	IC50 µM65.4	[69]
	(-)-hardwickiic acid (91)		38.9
	2β-acetoxyhardwickiic acid (201)		16.1
	2β-hydroxyhardwickiic acid (202)		82.4
	15-methoxypatagonic acid (203)		28.9
Nepeta suavis.	Nepetolide (204)	Carrageenan-induced hind paw edema Docking Analysis In silico evaluation	Compound 204 inhibited hind paw edemaTarget Cox-2 EGFR and Lox-2	[70]
Polyalthia longifolia	16-oxo-cleroda-3,13(14)E-dien-15-oic acid (205)	Cyclooxygenase inhibitory assay 5-LOX kitCOX-1 COX-25-LOX	IC50 µM8.008.418.41	[40,71]
	16-hydroxy-cleroda-3,13-dien-15-oic acid (206)	COX-1 COX-25-LOX	9.754.079.78
	16-hydroxy-cleroda-4(18),13-dien-16,15-olide (74)	COX-1 COX-25-LOX	3.772.714.06
	3α,16α-dihydroxy-cleroda-4(18),13(14)Z-dien-15,16-olide (75)	COX-1 COX-25-LOX	3.634.295.67
	16α-hydroxy-cleroda-3,13(14)Z-dien-15,16-olide (76)	COX-1 COX-25-LOX	3.013.294.58
		Docking Analysis In silico evaluation	Compounds 74–76 have interactions with COX-1/2 and LOX enzymes
	3β-16a-dihydroxy-cleroda-4(18),13(14)Z-dien-15,16-olide (77)	ELISA assayQuantification of cytokines such as TNF-α, TGF-β, IL-6, IL-10 and IL-1β	Compounds 74 and 77 inhibited production of proinfammatory cytokines and increased IL-10 and TGF-β
		Docking Analysis In silico evaluation	Compound 74 docked into the active sites of MDM2, TNF-α, FAK and IL-6Compound 77 docked into the active sites of MDM2, TNF-α, TGF-β and FAK
Scutellaria barbata	Scuttenline C (207)	Griess assayRAW264.7 macrophages stimulated LPS	IC50 μM1.9	[72]
	Barbatin A (208)		12.6
	Scutebarbatine F (209)		3.7
Teucrium fructicans	11-hidroxyfruticolone (210)	Griess assayRAW264.7 macrophages stimulated LPS	IC50 μM39.3	[73]
Tinospora crispa	Crispinoid D (211)	qPCR assayIL-1β, IL-6, TNF-α, iNOs, CCL12 and COX-2	Compounds 211–213 diminish the production of pro-inflammatory mediators	[74,75]
		Luciferase assay:Inhibition of NF-κB	IC50 μM5.94
	Tinosporol C (212)	Inhibition of NF-κB	6.32
	marrubiagenin-methylester (213)	Inhibition of NF-κB	25.20
	Tinopanoid A (214)	Griess assayBV-2 cells stimulated LPS	IC50 μM>60
	Tinopanoid B (215)		>60
	Tinopanoid C (216)		24.1
	Tinopanoid D (217)		41.1
	Tinopanoid E (218)		7.5
	Tinopanoid F (219)		50.8
	Tinopanoid G (220)		10.6
	Tinopanoid H (221)		39.4
	Tinopanoid I (222)		59.1
	Tinopanoid J (223)		45.9
	Tinospin C (224)		>60
	borapetol B (225)		>60
	Tinotufolin D (226)		14.5
Tinospora sagittata	Fibaruretin H (227)	Griess assayRAW264.7 macrophages stimulated LPS	% inhibition at 24 µM27.0%	[76]
	Fibaruretin I (228)		33.1%

Compound 166 (4aR,5S,6R,8aR)-5-[2-(2,5-dihydro-5-methoxy-2-oxofuran-3-yl)ethyl]-3,4,4a,5,6,7,8,8a-octahydro-5,6,8a-trimethylnaphthalene-1-carboxylic acid); Compound 170 (methyl (4aR,5S,6R,8S,8aR)-3,4,4a,5,6,7,8,8a-octahydro-8-hydroxy-5,6,8a-trimethyl-5-[2-(2-oxo-2,5-dihydrofuran-3-yl)ethyl]naphthalene-1-carboxylate); Compound 173 (3S,4S,6S,8R,9R,12S)-3-acetoxy-18-methoxycarbonyl-6,19:15,16-diepoxy-halim-5(10),13(16),14-triene-20,12-olide; Compound 174 (3S,4S,6S,8R,9R,12S)-3,19-diacetoxy-18-methoxycar-bonyl-15,16-epoxy-6-hydroxyhalim-5(10),13(16),14-triene-20,12-olide; Compound 191 (3,4,15,16-diepoxycleroda-13(16),14-diene-12,17-olide); Compound 192 (15,16-epoxy-3β-hydroxy-5(10),13(16),14-dien-12,17-olide; Compound 195 (3β,4β:15,16-diepoxy-13(16),14-clerodadiene; Compound 226 (2aβ,3α,5aβ,6β,7α,8aα)-6-[2-(3-furanyl)ethyl]-2a,3,4,5,5a,6,7,8,8a,8b-decahydro-2a,3-dihydroxy-6,7,8b-trimethyl-2H-naphtho[1,8-bc]furan-2-one). Cells are immortalized by v-raf/v-myc carrying J2 retrovirus (BV-2); inducible nitric oxide synthase (iNOS); cyclooxygenase 2 (COX-2); key sensor molecule in the inflammasome activity (NLRP3); protein found on the surface of some cells that binds epidermal growth factor (EGFR); 5-lipoxigenasa (5-LOX); tumor necrosis factor-α (TNF-α); interleukin-6 (IL-6); interleukin 1β (IL-1β); proinflammatory–chemokine (C-C motif) ligand 12 (CCL12).

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