The Study of the Composition of Chloroform Fraction of Caltha palustris

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ABSTRACT:

The analysis presented in this article is a continuation of a broader qualitative study examining the composition of the chloroform fraction of Caltha palustris. The study discovered 29 compounds, and 26 of them have been identified:paraffinic hydrocarbons, esters, fatty aldehydes, morphine derivatives, thiazole derivatives, benzodiazepines, naphthalene, and a small amount of terpene. It was figured out that the investigated lipophilic extract has an antioxidant impact. For the investigation, there were used such assays as the DPPH radical and the ABTS radical cation assays. Moreover the investigated lipophilic extract has an antimicrobial activity both in terms of gram-positive (Staphylococcus aureus (ATCC 25923 (F-49)), Bacillus cereus 34 (non-MDR), Enterococcus faecalis 26(MDR) and gram-negative, (Raoultella terrigena (ATCC 33257), Escherichia coli (ATCC 25922), Escherichia coli 168) microorganisms and yeast Candida spp. In addition, the significant content of biologically active substances in the lipophilic volatile fraction of Caltha palustris indicates the prospects for further study.

KEYWORDS: Caltha palustris, Chloroform fraction, Gas chromatography, Lipophilic extract, Antioxidant effect, Antimicrobial activity.

INTRODUCTION:

Over the recent years, we can observe an increasing interest in herbs. Due to this, it is necessary to pay attention to understanding different aspects of phytoremedies production and their practical implementation.

One of the most topical and promising species of the Ukrainian flora to be used in contemporary medicine and pharmacology is Caltha palustris in the buttercup family (Ranunculaceae).

This plant is widely used in complementary and alternative medicine as an anti-inflammatory, antispasmodic, bactericidal, antimicrobial, analgesic, and diuretic agent.

To the main biologically active compounds, there belong tannins, glycosides (protoanemonin and anemonin y-lactones) saponins, berberine, bitterance, vitamin C, choline, carotene, flavonoids, and alkaloids1,2.

The main area for Caltha palustris spread is the highlands of the Ukrainian Carpathians. At the same time, the plant is rarely grown in the other administrative territories of Ukraine3.

Taking into consideration the broad experience of the plant implementation in conventional and alternative medicine, and partially official medicine4-6, its wide spectrum of implementation in pharmacology, its content, and biologically active compounds7-9 together with the results of phytochemical and pharmacological investigations, it is possible to understand that the further use of Caltha palustris is an up-to-day task both for pharmacology and pharmaceutic biotechnology due to the perspective of investigations on new phytoremedies development and implementation10.