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© 2023 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.

Abstract

Induced by the spread of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), the COVID-19 pandemic underlined the clear need for antivirals against coronaviruses. In an effort to identify new inhibitors of SARS-CoV-2, a screening of 824 extracts prepared from various parts of 400 plant species belonging to the Rutaceae and Annonaceae families was conducted using a cell-based HCoV-229E inhibition assay. Due to its significant activity, the ethyl acetate extract of the leaves of Clausena harmandiana was selected for further chemical and biological investigations. Mass spectrometry-guided fractionation afforded three undescribed phenolic lipids (13), whose structures were determined via spectroscopic analysis. The absolute configurations of 1 and 2 were determined by analyzing Mosher ester derivatives. The antiviral activity against SARS-CoV-2 was subsequently shown, with IC50 values of 0.20 and 0.05 µM for 2 and 3, respectively. The mechanism of action was further assessed, showing that both 2 and 3 are inhibitors of coronavirus entry by acting directly on the viral particle. Phenolic lipids from Clausena harmandiana might be a source of new antiviral agents against human coronaviruses.

Details

Title
New Phenolic Lipids from the Leaves of Clausena harmandiana Inhibit SARS-CoV-2 Entry into Host Cells
Author
Chambon, Marion 1   VIAFID ORCID Logo  ; Herrscher, Charline 2   VIAFID ORCID Logo  ; Dana Al Halabi 2 ; Nathan, François 3 ; Belouzard, Sandrine 3   VIAFID ORCID Logo  ; Boutet, Stéphanie 4   VIAFID ORCID Logo  ; Pham, Van Cuong 5   VIAFID ORCID Logo  ; Thi Mai Huong Doan 5   VIAFID ORCID Logo  ; Séron, Karin 3   VIAFID ORCID Logo  ; Mavingui, Patrick 2   VIAFID ORCID Logo  ; Litaudon, Marc 1   VIAFID ORCID Logo  ; Chaker El Kalamouni 2   VIAFID ORCID Logo  ; Apel, Cécile 1   VIAFID ORCID Logo 

 Institut de Chimie des Substances Naturelles, CNRS, UPR 2301, Université Paris-Saclay, 91198 Gif-sur-Yvette, France; [email protected] 
 Unité Mixte Processus Infectieux en Milieu Insulaire Tropical, Université de la Réunion, INSERM U1187, CNRS UMR 9192, IRD UMR 249, Plateforme Technologique CYROI, 94791 Sainte Clotilde, France; [email protected] (C.H.); [email protected] (D.A.H.); [email protected] (P.M.) 
 Center for Infection and Immunity of Lille (CIIL), Institut Pasteur de Lille, Université de Lille, INSERM U1019, CNRS UMR 8204, CHU Lille, 59000 Lille, France; [email protected] (N.F.); [email protected] (S.B.); [email protected] (K.S.) 
 Institut Jean-Pierre Bourgin (IJPB), AgroParisTech, INRAE, Université Paris-Saclay, 78000 Versailles, France; [email protected] 
 Institute of Marine Biochemistry, Vietnam Academy of Science and Technology (VAST), 18 Hoang Quoc Viet, CauGiay, Hanoi 10072, Vietnam; [email protected] (V.C.P.); [email protected] (T.M.H.D.) 
First page
5414
Publication year
2023
Publication date
2023
Publisher
MDPI AG
e-ISSN
14203049
Source type
Scholarly Journal
Language of publication
English
ProQuest document ID
2843094038
Copyright
© 2023 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.