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© 2023, Schepler-Luu, Sciallano, Stiebner et al. This work is published under https://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.

Abstract

Bacterial leaf blight (BB) of rice, caused by Xanthomonas oryzae pv. oryzae (Xoo), threatens global food security and the livelihood of small-scale rice producers. Analyses of Xoo collections from Asia, Africa and the Americas demonstrated complete continental segregation, despite robust global rice trade. Here, we report unprecedented BB outbreaks in Tanzania. The causative strains, unlike endemic African Xoo, carry Asian-type TAL effectors targeting the sucrose transporter SWEET11a and iTALes suppressing Xa1. Phylogenomics clustered these strains with Xoo from Southern-China. African rice varieties do not carry effective resistance. To protect African rice production against this emerging threat, we developed a hybrid CRISPR-Cas9/Cpf1 system to edit all known TALe-binding elements in three SWEET promoters of the East African elite variety Komboka. The edited lines show broad-spectrum resistance against Asian and African strains of Xoo, including strains recently discovered in Tanzania. The strategy could help to protect global rice crops from BB pandemics.

Details

Title
Genome editing of an African elite rice variety confers resistance against endemic and emerging Xanthomonas oryzae pv. oryzae strains
Author
Schepler-Luu Van; Coline, Sciallano; Stiebner Melissa; Ji Chonghui; Boulard Gabriel; Diallo Amadou; Auguy Florence; Char Si Nian; Arra Yugander; Schenstnyi Kyrylo; Buchholzer Marcel; Loo Eliza PI; Bilaro, Atugonza L; Lihepanyama, David; Mkuya Mohammed; Murori Rosemary; Oliva, Ricardo; Cunnac Sebastien; Yang, Bing; Szurek Boris; Frommer, Wolf B
University/institution
U.S. National Institutes of Health/National Library of Medicine
Publication year
2023
Publication date
2023
Publisher
eLife Sciences Publications Ltd.
e-ISSN
2050084X
Source type
Scholarly Journal
Language of publication
English
ProQuest document ID
2844048901
Copyright
© 2023, Schepler-Luu, Sciallano, Stiebner et al. This work is published under https://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.