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Abstract. A spherical green microalga, namely Parachlorella kessleri AUP5, was isolated from freshwater ponds of Andalas University, Padang, Indonesia and further organic solvents extracts were realised to study antioxidant and a-glucosidase inhibitor activities of this microalga. The studies demonstrated radical scavencing 2,2-diphenyl-1-picrylhydrazyl (DPPH) metahnol extract was found to be 54.64 ± 0.8% at 500 qg ml-1 with a total phenolic content of 84.22 mg (GAE) g-1. In the a-glucosidase screening inhibition assay of Parachlorella kessleri AUP5, hexane extract was found to be 91.97±0.29 % which was the highest a-glucosidase inhibition followed by ethyl acetate and methanol extract in amount of 84.37±1.89 % and 84.27±0.89 %, at concentration of 250 pg ml-1 respectively. Hexane extracts were subsequently characterized using gas chromatography-mass spectrometry for identifying bioactive compounds. The former was found to be enriched with 2,6-Octadien-1-ol, 3,7-dimethyl-acetate (E) (37.53%), 9,12-Octadecadienoic acid (29.25%), and tetradecanoic acid (7%). The presence of these bioactive compounds in Parachlorella kessleri AUP5 might make it beneficial as a source of a-glucosidase inhibitor agents. Thus, our findings indicate that freshwater microalgae, namely Parachlorella kessleri AUP5 has potential to be a source of promising antioxidant and antihyperglycemic therapeutic agents.
Key Words: microalgae, identification, phenolic.
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Introduction. Microalgae are photosynthetic microorganisms which are a potential source of bioactive compounds that can be used for various purposes in pharmaceuticals, cosmetics, bioenergy and bioremediation (Dębowski et al 2020; Perdana et al 2021; Zhang et al 2014). The properties of natural bioactive compounds from microalgae which have made them promising sources for discovering natural compounds are proteins, fatty acids, carbohydrates, chlorophylls, phenolic compounds, and carotenoids (Fu et al 2017; Kreckhoff et al 2019; Wali et al 2020). These bioactive compounds may have great potential for therapeutic activities such antioxidant and cytotoxic activities (Hussein et al 2019; Rodrigues et al 2012), antifungal (Nainangu et al 2020), anti-inflammatory (Lauritano et al 2016), and antidiabetic activity (Kawee-Ai et al 2019). Microalgae grow rapidly and do not compete directly with crops for agricultural land in the process of cultivation (Nuhma et al 2021; Santhakumaran et al 2020). Therefore, efforts to study microalgae as a source of bioactive compounds for pharmaceuticals discovery became an important research direction.
In the last decades, increased attention for microalgae-based pharmaceutical and nutraceutical products...