Abstract

Aflatoxin B1 (AFB1) poses a major risk to both human and animal health because it contaminates food, feed, and grains. These dangerous effects can be mitigated using natural components. The purpose of this study was to examine the ameliorative effects of camel milk and silymarin supplementation upon aflatoxin B1 induced hepatic injury in rats. This improvement was assessed by measuring leukocytic and deferential counts, serum biochemical parameters, and gene expression of Tumor Necrosis Factor (TNF-α), antioxidant gene (NAD(P)H quinone oxidoreductase 1 (NQO1)), and base excision repair genes (APE1 and OGG1) in the liver tissue, in addition to liver histopathology. Sixty mature males Wister white rats were used to perform the present study; the rats were distributed in six groups (ten rats/group). The control group (without any treatment) received saline by gavage. The camel milk group received 1 ml of camel milk/kg body weight. The silymarin group received 1 ml of silymarin suspension solution at a dose of 20 mg of silymarin/kg of b.wt. The aflatoxin group received an aflatoxin-contaminated diet at a dose of 1.4 mg of aflatoxin /kg of diet and received saline. The camel milk + aflatoxin group received the same previous oral doses of camel milk and an aflatoxin-contaminated diet at the same time. The silymarin + aflatoxin group received the same previous doses of silymarin orally and an aflatoxin-contaminated diet at the same time. The obtained data indicated the deleterious effect of aflatoxin B1 on the leukocytic count, activity of AST and ALT, serum proteins, ferritin, alpha-fetoprotein, carcinoembryonic antigen, liver pathology, and the expression of the studied genes. However, these deleterious effects were mitigated by camel milk and silymarin supplementation. Thus, we could conclude that the ingestion of camel milk and silymarin mitigated the negative effects of AFB1 on the hematology, activity of AST and ALT, serum proteins, ferritin, alpha-fetoprotein, carcinoembryonic antigen, liver pathology, and gene expression in the rat model.

Details

Title
Ameliorative effects of camel milk and silymarin upon aflatoxin B1 induced hepatic injury in rats
Author
Hassaneen, Nahla H. 1 ; Hemeda, Shabaan A. 2 ; El Nahas, Abeer F. 2 ; Fadl, Sabreen E. 3 ; El-diasty, Eman M. 4 

 Matrouh University, Department of Animal Husbandry and Animal Wealth Development, Faculty of Veterinary Medicine, Matrouh, Egypt 
 Alexandria University, Department of Animal Husbandry and Animal Wealth Development, Faculty of Veterinary Medicine, Alexandria, Egypt (GRID:grid.7155.6) (ISNI:0000 0001 2260 6941) 
 Matrouh University, Biochemistry Department, Faculty of Veterinary Medicine, Matrouh, Egypt (GRID:grid.7155.6) 
 Animal Health Research Institute (ARC), Mycology and Mycotoxins Department, Giza, Egypt (GRID:grid.418376.f) (ISNI:0000 0004 1800 7673) 
Pages
15092
Publication year
2023
Publication date
2023
Publisher
Nature Publishing Group
e-ISSN
20452322
Source type
Scholarly Journal
Language of publication
English
ProQuest document ID
2864019877
Copyright
© The Author(s) 2023. This work is published under http://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.