Abstract

ABSTRACT

Genomes of strains of the zoophilic dermatophyte Microsporum canis from invasive (disseminated and subcutaneous) and noninvasive (tinea capitis) infections were compared. Especially the disseminated strain showed significant syntenic rearrangements, including multiple translocations and inversions, and numerous SNPs and Indels in comparison to the noninvasive strain. In transcriptome analysis, both invasive strains were enriched for GO pathways related to components of the membrane, iron binding and heme binding, which possibly enables them to invade deeper into dermis and blood vessels. At 37 °C, invasive strains showed gene expression enriched for DNA replication, mismatch repair, N-glycan biosynthesis and ribosome biogenesis. The invasive strains were slightly less susceptible to multiple antifungal agents suggesting that acquired elevated drug resistance might be involved in the refractory disease courses. Patient with disseminated infection failed to respond to a combined antifungal treatment with itraconazole, terbinafine, fluconazole and posaconazole.

Details

Title
Comparative analysis of whole genomes and transcriptomes of Microsporum canis from invasive dermatophytosis and tinea capitis
Author
Wang, Ruojun 1 ; Liu, Weixia 2 ; Liu, Xiao 3 ; Wan, Zhe 1 ; G. Sybren de Hoog 4 ; Li, Ruoyu 1 ; Song, Yinggai 5 

1  Department of Dermatology and Venerology, Peking University First Hospital , Beijing , People’s Republic of China, Research Center for Medical Mycology, Peking University , Beijing , People’s Republic of China, National Clinical Research Center for Skin and Immune Diseases , Beijing , People’s Republic of China, Beijing Key Laboratory of Molecular Diagnosis on Dermatoses , Beijing , People’s Republic of China 
2  Department of Dermatology and Venerology, Peking University First Hospital , Beijing , People’s Republic of China, Department of Dermatology, Heping Hospital Affiliated to Changzhi Medical College , Changzhi , People’s Republic of China 
3  Department of Dermatology and Venerology, Peking University First Hospital , Beijing , People’s Republic of China, Department of Dermatology and Venerology, Beijing Jishuitan Hospital , Beijing , People’s Republic of China 
4  Department of Dermatology and Venerology, Peking University First Hospital , Beijing , People’s Republic of China, Center of Expertise in Mycology of Radboud University Medical Center/Canisius Wilhelmina Hospital , Nijmegen , The Netherlands 
5  Department of Dermatology and Venerology, Peking University First Hospital , Beijing , People’s Republic of China, Research Center for Medical Mycology, Peking University , Beijing , People’s Republic of China, National Clinical Research Center for Skin and Immune Diseases , Beijing , People’s Republic of China, Beijing Key Laboratory of Molecular Diagnosis on Dermatoses , Beijing , People’s Republic of China, Center of Expertise in Mycology of Radboud University Medical Center/Canisius Wilhelmina Hospital , Nijmegen , The Netherlands 
Publication year
2023
Publication date
Dec 2023
Publisher
Taylor & Francis Ltd.
e-ISSN
22221751
Source type
Scholarly Journal
Language of publication
English
DOI
https://doi.org/10.1080/22221751.2023.2219346
ProQuest document ID
2867483696
Copyright
© 2023 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group, on behalf of Shanghai Shangyixun Cultural Communication Co., Ltd. This work is licensed under the Creative Commons Attribution License http://creativecommons.org/licenses/by/4.0/ (the "License"). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.