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Abstract

C-phycocyanin is a high market value compound derived from cyanobacterium Spirulina platensis and has a wide range of applications in pharmaceutical and food industries as a useful biochemically active compound. In this study, essentially an aqueous two-phase system (ATPS) is presented for purification of C-phycocyanin. The phase behavior of ATPSs that include Pluronic copolymers and salts was evaluated systematically. After that, the effects of different parameters such as salt type, copolymer structure, pH of solution, tie line length (TLL), system’s temperature, and volume ratio for purification of C-phycocyanin were investigated. The results revealed that Pluronic 10R5/potassium phosphate buffer system is the most appropriate system for promoting the C-phycocyanin separation from other contaminants. Besides, the purity index of C-phycocyanin was enhanced by up to 3.92 at TLL of 46.31%, pH = 6, volume ratio of 0.34, and temperature of 35 °C without any loss of stability. At these conditions, the obtained C-phycocyanin recovery was 90%. After that, the purity factor reached to 5.9 by applying the ultrafiltration method. In addition, the results of circular dichroism (CD) spectroscopy proved that the C-phycocyanin structure remained intact during the purification step. Finally, 78% of 10R5 copolymer was recovered by increasing the temperature above 57 °C (micelle formation (which can be used in new ATPS.

Details

Title
Separation and Purification of C-Phycocyanin from Spirulina platensis Using Aqueous Two-Phase Systems Based on Triblock Thermosensitive Copolymers
Author
Ebrahimi, Alireza 1 ; Pazuki, Gholamreza 1 ; Mozaffarian, Mehrdad 1 ; Ahsaie, Farzaneh Ghazizadeh 1 ; Abedini, Hamed 2 

1  Amirkabir University of Technology (Tehran Polytechnic), Department of Chemical Engineering, Tehran, Iran (GRID:grid.411368.9) (ISNI:0000 0004 0611 6995) 
2  KU Leuven, Mechanical Engineering Department, Louvain, Belgium (GRID:grid.5596.f) (ISNI:0000 0001 0668 7884) 
Pages
2582-2597
Publication year
2023
Publication date
Nov 2023
Publisher
Springer Nature B.V.
ISSN
19355130
e-ISSN
19355149
Source type
Scholarly Journal
Language of publication
English
DOI
https://doi.org/10.1007/s11947-023-03057-6
ProQuest document ID
2870189625
Copyright
© The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature 2023. Springer Nature or its licensor (e.g. a society or other partner) holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law.