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Abstract
Plasmodium falciparum secretes extracellular vesicles (PfEVs) that contain parasite-derived RNA. However, the significance of the secreted RNA remains unexplored. Here, we compare secreted and intracellular RNA from asexual cultures of six P. falciparum lines. We find that secretion of RNA via extracellular vesicles is not only periodic throughout the asexual intraerythrocytic developmental cycle but is also highly conserved across P. falciparum isolates. We further demonstrate that the phases of RNA secreted via extracellular vesicles are discernibly shifted compared to those of the intracellular RNA within the secreting whole parasite. Finally, transcripts of genes with no known function during the asexual intraerythrocytic developmental cycle are enriched in PfEVs compared to the whole parasite. We conclude that the secretion of extracellular vesicles could be a putative posttranscriptional RNA regulation mechanism that is part of or synergise the classic RNA decay processes to maintain intracellular RNA levels in P. falciparum.
Here, Kioko et al. describe a putative posttranscriptional RNA regulation mechanism involving secreted extracellular vesicles to maintain the intracellular steady-state RNA levels during the asexual blood stage of malaria parasites.
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1 KEMRI-Wellcome Trust Research Programme, Bioscience Department, Kilifi, Kenya (GRID:grid.33058.3d) (ISNI:0000 0001 0155 5938); Open University, Milton Keynes, UK (GRID:grid.10837.3d) (ISNI:0000 0000 9606 9301)
2 Wellcome Sanger Institute, Pathogens and Microbes Programme, Cambridge, UK (GRID:grid.10306.34) (ISNI:0000 0004 0606 5382); University of Hertfordshire, School of Life and Medical Science, Hatfield, UK (GRID:grid.5846.f) (ISNI:0000 0001 2161 9644)
3 KEMRI-Wellcome Trust Research Programme, Bioscience Department, Kilifi, Kenya (GRID:grid.33058.3d) (ISNI:0000 0001 0155 5938)
4 Wellcome Sanger Institute, Pathogens and Microbes Programme, Cambridge, UK (GRID:grid.10306.34) (ISNI:0000 0004 0606 5382)
5 University of Cambridge, Cambridge Institute of Medical Research, Cambridge, UK (GRID:grid.5335.0) (ISNI:0000 0001 2188 5934)
6 KEMRI-Wellcome Trust Research Programme, Bioscience Department, Kilifi, Kenya (GRID:grid.33058.3d) (ISNI:0000 0001 0155 5938); Pwani University Biosciences Research Centre, Pwani University, Kilifi, Kenya (GRID:grid.449370.d) (ISNI:0000 0004 1780 4347)
7 KEMRI-Wellcome Trust Research Programme, Bioscience Department, Kilifi, Kenya (GRID:grid.33058.3d) (ISNI:0000 0001 0155 5938); University of Oxford, Centre for Tropical Medicine and Global Health, Nuffield Department of Medicine, Oxford, UK (GRID:grid.4991.5) (ISNI:0000 0004 1936 8948)
8 KEMRI-Wellcome Trust Research Programme, Bioscience Department, Kilifi, Kenya (GRID:grid.33058.3d) (ISNI:0000 0001 0155 5938); Pwani University Biosciences Research Centre, Pwani University, Kilifi, Kenya (GRID:grid.449370.d) (ISNI:0000 0004 1780 4347); University of Oxford, Centre for Tropical Medicine and Global Health, Nuffield Department of Medicine, Oxford, UK (GRID:grid.4991.5) (ISNI:0000 0004 1936 8948)