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Human dental pulp stem cells (DPSCs) exhibit multilineage differentiation capabilities and superior clonogenic and proliferative properties. However, the use of animal-derived components such as FBS raises concerns regarding the clinical application of stem-cell-based therapies. Platelet-rich fibrin (PRF) derived from human blood is rich in fibrin, platelets, and growth factors and acts as a bioactive scaffold for grafting with biomaterials. In this study, we assessed the efficacy of PRF-conditioned medium (CM) in promoting DPSCs proliferation and osteogenic differentiation compared with the standard culture medium supplemented with FBS. A comparison of DPSCs cultured in FBS and PRF-CM revealed no differences in characteristics or morphology. However, cells cultured with PRF-CM exhibited inferior proliferation rates and cell numbers during passage in comparison with those cultured with FBS. In contrast, DPSCs cultured in PRF-CM showed significantly higher levels of calcification, and RT-PCR confirmed that the gene expression levels of markers associated with osteoblast differentiation were significantly increased. The PRF-CM approach offers a convenient, straightforward, and advantageous method for culturing DPSCs, without relying on animal-derived components. In summary, this study introduces a novel application of PRF-CM for enhancing the osteogenesis of DPSCs, which provides an alternative to FBS culture medium and addresses concerns associated with the use of animal-derived components in clinical settings.
Details
Fibrin;
Cell culture;
Leukocytes;
Fetal calf serum;
Cytology;
Cell proliferation;
Stem cells;
Blood platelets;
Anticoagulants;
Cell differentiation;
Differentiation (biology);
Biomedical materials;
Biomaterials;
Blood;
Growth factors;
Dental pulp;
Consent;
Physical characteristics;
Culture media;
Culture;
Osteogenesis;
Gene expression;
Calcification;
Osteoblastogenesis;
Medical research;
Platelets;
Cell growth;
Mesenchymal stem cells;
Bioengineering
; Yamakawa, Daiki 1 ; Al-Maawi, Sarah 2 ; Dohle, Eva 2 ; Jin Chikira 1 ; Fujii, Yasuyuki 1 ; Miki, Megumu 1 ; Sader, Robert 2 ; Chikazu, Daichi 1 ; Ghanaati, Shahram 2
; Kawase-Koga, Yoko 3 1 Department of Oral and Maxillofacial Surgery, Tokyo Medical University, 6-7-1 Nishishinjuku, Shinjuku-ku, Tokyo 160-0023, Japan;
2 FORM, Frankfurt Oral Regenerative Medicine, Clinic for Maxillofacial and Plastic Surgery, Goethe University, Theodor-Stern-Kai 7, 60596 Frankfurt, Germany
3 Department of Oral and Maxillofacial Surgery, Tokyo Medical University, 6-7-1 Nishishinjuku, Shinjuku-ku, Tokyo 160-0023, Japan;